Multi-site enzymatic repairing amplification (MSERA) enables ultrasensitive detection of terminal deoxynucleotidyl transferase activity.

A novel multi-site enzymatic repairing amplification strategy is developed for high-sensitive terminal deoxynucleotidyl transferase quantification through combining enzymatic repairing amplification and lesion base-involved terminal extension. This method may provide a sensitive and flexible tool for molecular diagnosis and drug discovery.

Wearable heart rate variability analysis system based on ionic conductive hydrogels.

Developing a wearable device that can continuously and reliably detect and evaluate heart rate variability (HRV) parameters is critical for the diabetic population with cardiac autonomic neuropathy (CAN). In this work, we proposed a zwitterionic conducting hydrogel that enabled a reliable and comfortable wearable device for the evaluation and detection of the autonomic nervous system (ANS). The hydrogel can achieve a strain of 2003 %, an electrical conductivity of 190 mS/m, and is capable of adhering to a variety of materials, including wood, plastic, and glass.

[Distribution characteristics and heritability of alcohol consumption behavior in adult twins in China].

To describe the distribution characteristics of alcohol consumption in adult twins in the Chinese National Twin Registry (CNTR), and further explore the influence of genetic factors on alcohol consumption in adult twins. The subjects of the study were twins registered by CNTR in 11 project areas across China from 2010 to 2018. A total of 56 966 twins (28 483 pairs) aged 18 years and above who answered questions about drinking behavior were included, and the random effect model was used to describe the population and regional distribution characteristics of alcohol consumption.

Association between obesity and thyroid nodules in children and adults living in iodine-sufficient areas: analysis of cross-sectional data from Tianjin, China.

Objective: The main purpose of this study was to analyse the association between obesity and thyroid nodules in children and adults living in iodine-sufficient areas in China.

Design: Analysis of data from two cross-sectional surveys.

Setting And Participants: 921 children from 2016 to 2021 and 1505 adults from 2018 to 2021 living in iodine-sufficient areas from Tianjin, China were recruited.

The efficacy and safety of robot-assisted surgery in cancer patients: a systematic review of randomised controlled trials.

Background: The utilization of robot-assisted surgery (RAS) has been increasing among cancer patients. However, evidence supporting the use of RAS remains uncertain. The availability of randomized controlled trials (RCTs) for each surgical procedure is limited.

Molecular Epidemiology, Transmission, and Evolution of Rubella Virus Circulating in Tianjin, China, Between 2009 and 2020.

Rubella is listed as a disease that needs to be eliminated worldwide by the World Health Organization. This study aimed to investigate rubella epidemiology and genetic characteristics based on data from 12 years of laboratory-based surveillance (2009-2020) in Tianjin and to provide baseline genotype data for monitoring future rubella control efforts. We collected RV-positive throat swab samples from confirmed rubella cases during 2009-2020 in Tianjin to isolate RV, amplify and sequence target gene fragments, construct phylogenetic trees, and analyze nucleotide homologies.

[Construction of evidence graph for modifiable risk factors for diabetic retinopathy].

Diabetic retinopathy (DR) has been reported as the leading cause of blindness among diabetic adults, which is closely related to poor quality of life and increased burden of disability. This study aimed to aggregate the optimally available evidence on modifiable risks of DR. Until June 2023, PubMed, Cochrane Library, CNKI, and Wanfang databases were used to retrieve Meta-analysis about various risk factors for DR, and Meta-analysis were analyzed and summarized.

[Analysis of human papillomavirus infection status and related factors among men who have sex with men in Tianjin].

The status quo and related factors of human papillomavirus (HPV) infection in multiple parts of men who have sex with men (MSM) in Tianjin were analyzed. Using the cross-sectional survey method, a total of 600 MSM were recruited in Tianjin from September to December 2023, including 200 (18-26 years old), 300 (27-45 years old), and 100 (≥46 years old) stratificaed by age group. Questionnaire survey was used to collect knowledge and behavioral information related to demography and STD prevention and treatment.

[Survey on diarrhea symptoms and diarrhea vaccination willingness of residents in Tianjin].

To understand the symptoms of diarrhea of residents in Tianjin and their willingness to vaccinate, to provide reference for health education, prevention and control of infectious diarrhea. Cross-sectional study design was adopted. From September to December 2023, a questionnaire survey was conducted among some residents as subjects in Nankai District, Beichen District, Baodi District and Binhai Area of Tianjin by multistage stratified cluster random sampling,to collect information such as sociodemographic characteristics, diarrhea symptoms and vaccination intention.

[Visualization analysis of literature on sudden poisoning incidents in China from 2013 to 2022 based on CiteSpace and VOSviewer].

To use CiteSpace and VOSviewer software to perform visualization analysis on research literature on sudden poisoning incidents in China from 2013 to 2022, in order to grasp the research hotspots and priorities, current research development status and trend of sudden poisoning incidents, and provide reference and ideas for subsequent research. In October 2023, literature related to sudden poisoning incidents was searched using the China National Knowledge Infrastructure and Wanfang databases. The bibliometric analysis software CiteSpace 6.

Roles of mTOR-p70S6K signaling pathway and HO-1 in ethylbenzene-induced hepatoxic effects in L02 cells.

Ethylbenzene (EB)-induced hepatotoxic effects has been indicated as oxidative damage and mitochondria-mediated apoptosis in vivo in our previous study, yet the mechanisms remain unclear. This study aimed to explore the role of the mTOR-p70S6K signaling pathway in EB-induced hepatoxic effects in vitro. Normal human hepatocytes (L02 cells) were exposed to different concentrations of ethylbenzene (0-10 mM) for 24 h.

[Analysis of factors affecting health status and abnormal lung function of workers exposed to welding fumes in Tianjin from 2020 to 2022].

To investigate the health status and the influencing factors of abnormal lung function of welding fumes exposure workers in Tianjin from 2020 to 2022, and to provide theoretical basis for the protection and intervention of key occupational diseases. In October 2023, the relevant data of occupational health examination of welding fumes exposure workers in Tianjin from 2020 to 2022 (10831, 10758 and 21717 in 2020, 2021 and 2022, respectively) were collected from the China Disease Prevention and Control Information Platform. The electrocardiogram, chest radiograph and lung function abnormalities of workers in each year were analyzed, as well as the association between lung function abnormalities and gender, age, working age, region, etc.

Analysis of multi-site HPV infection and vaccination willingness among men who have sex with men in Tianjin, China.

Background: Men who have sex with men (MSM) are vulnerable to HPV infection. This study aims to explore the HPV infection status at different sites among HIV-positive MSM, HIV-negative MSM, and men who have sex with women (MSW), and to investigate their willingness to receive HPV vaccination.

Methods: From September 2023 to April 2024, three groups were recruited in Tianjin, China.

Amplification-free miRNA detection with CRISPR/Cas12a system based on fragment complementary activation strategy.

CRISPR/Cas12a systems have been repurposed as powerful tools for developing next-generation molecular diagnostics due to their -cleavage ability. However, it was long considered that the CRISPR/Cas12a system could only recognize DNA targets. Herein, we systematically investigated the intrinsic -cleavage activity of the CRISPR/Cas12a system (LbCas12a) and found that it could be activated through fragmented ssDNA activators.

Gut microbiota and inflammation analyses reveal the protective effect of medium-chain triglycerides combined with docosahexaenoic acid on cognitive function in APP/PS1 and SAMP8 mice.

Accumulating evidence has demonstrated that medium-chain triglycerides (MCTs) and docosahexaenoic acid (DHA) positively affect cognitive function. However, it remains unclear whether the improvement is related to the alterations of gut microbiota and inflammation and the impact of the combined intervention. In this study, we hypothesized that the supplementation of MCTs combined with DHA could modulate gut microbiota, inflammation, and improve cognitive function in APPswe/PS1De9 model mice and senescence-accelerated mouse-prone-8, which are two different mouse models used in neurodegeneration research.

The construction and validity assessment of the respiratory air quality health index (AQHI) based on the analytic hierarchy process in Tianjin, China.

Background: Air quality health index (AQHI), as a developed air quality risk communication tool, has been proved to be more accurate in predicting air quality related health risks than air quality index (AQI) by previous studies. However, the standard method to construct AQHI is summing the excess risks of single-pollutant models directly, which may ignore the joint effect of air pollutant mixtures.

Methods: In this study, a new method which could solve the aforementioned problem, Analytic hierarchy process (AHP), was introduced.

Spatial distribution and risk assessment of perchlorate in raw cow milk from China.

Perchlorate is a ubiquitous environmental contaminant worldwide, recognized as an emerging thyroid toxicant. This study focused on the pollution status, spatial distribution, possible sources of perchlorate in raw cow milk collected from 155 dairy farms in China, as well as the health risk of exposure to perchlorate through dairy products. The results showed that the detection rate of perchlorate in raw milk was 100% with the mean of 15.

Tocotrienol suppresses colitis-associated cancer progression through TLR4 signaling in a mouse model of colorectal cancer.

This study aimed to evaluate the preventive efficacy of tocotrienol in inhibiting the nuclear factor-kappa B (NF-κB) mediated inflammation pathways in colorectal cancer. We utilized the azoxymethane (AOM) and dextran sulfate sodium salt (DSS) to induce colitis-associated colorectal cancer (CAC) mice model. In generating a CAC model, mice were intraperitoneally injected with AOM at a concentration of 10 mg/kg body weight.

A highly elastic, moisturizing, and adhesion conductive hydrogel designed for cuffless blood pressure measurement.

Real-time monitoring of blood pressure in athletes during exercise is of great importance, however, conventional methods pose challenges in achieving portable and instantaneous measurements. While, the electrodes used in emerging wearable blood pressure monitoring instruments still have certain defects. In this study, a novel cuffless blood pressure monitoring system was developed using a self-developed polyacrylamide/trehalose/LiCl (PAM/Trehalose/LiCl) conductive hydrogel as the electrode material.