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Aim: To establish a sensitive biotin-avidin enzyme linked immunosorbent assay (BA-ELISA) method for detecting carcinoembryonic antigen (CEA) in serum.

Methods: CEA which had been purified by affinity chromatography was used to immunize the rabbits to produce polyclonal antibodies. Then the antibodies were connected with biotin and horseradish peroxidase (HRP).

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