VDAC1 Cleavage Promotes Autophagy in Renal Tubular Epithelial Cells With Hypoxia/Reoxygenation Injury.

Aim: To study the effect and elucidate the underlying mechanisms of VDAC1-ΔC on autophagy in renal tubular epithelial cells injured by hypoxia/reoxygenation.

Methods: C57/BL6 mice were randomly divided into groups: sham operation group, IRI 1d group and IRI 2d group. The inner canthal blood of mice was collected to detect the levels of serum creatinine and urea nitrogen and kidney tissues were sampled, and sections were stained with Periodic acid-Schiff for morphological evaluation.

An exceptionally large wave of infections among children in Tianjin post COVID-19 pandemic.

Background: The coronavirus disease 2019 (COVID-19) pandemic has caused a persistent lowering of (), which is commonly found in children with respiratory tract infections (RTIs). However, in 2023, we observed an unusually high number of infections among children from Tianjin, the second largest city in northern China. This study sought to analyze the epidemiological characteristics of children with RTIs caused by in northern China post COVID-19, in order to provide a theoretical basis for clinical diagnosis and treatment.

Complex delayed blood transfusion reaction: A case report.

Introduction: Inefficient blood transfusions present a significant challenge, leading to the wastage of crucial blood resources and increased medical expenses. This study aims to address this issue by providing a comprehensive analysis of a case involving an ineffective clinical transfusion and outlining the significance of identifying multiple alloantibodies in resolving transfusion challenges.

Case Report: We present a detailed follow-up on a patient treatment journey, highlighting the critical role of identifying multiple alloantibodies through various methodologies in addressing the transfusion problem.

Serology and molecular genetic analysis of two unrelated individuals with the same novel CisAB blood type.

Objective: This study aims to report two unrelated individuals with the same novel CisAB blood type and confirm this rare blood type using a comprehensive approach that combines serological and molecular biology techniques.

Methods: Peripheral blood samples were collected from two patients and their family members. ABO blood typing and antibody detection were performed using conventional tube methods.

Effects of heat waves and cold spells on blood parameters: a cohort study of blood donors in Tianjin, China.

Background: With the increasing occurrence of extreme temperature events due to climate change, the attention has been predominantly focused on the effects of heat waves and cold spells on morbidity and mortality. However, the influence of these temperature extremes on blood parameters has been overlooked.

Methods: We conducted a cohort study involving 2,752 adult blood donors in Tianjin, China, between January 18, 2013, and June 25, 2021.

A Dynamic Membrane-Active Glycopeptide for Enhanced Protection of Human Red Blood Cells against Freeze-Stress.

Intracellular delivery of freezing-tolerant trehalose is crucial for cryopreservation of red blood cells (RBCs) and previous strategies based on membrane-disruptive activity usually generate severe hemolysis. Herein, a dynamic membrane-active glycopeptide is developed by grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis. Results of the mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition toward membrane stabilization are essential for keeping cellular biofunctions.

Membrane stabilization perturbation by aromatic monoamine-modified γ-PGA for cryopreservation of human RBCs with high intracellular trehalose.

As a nonreducing disaccharide, trehalose can be used as a biocompatible cryoprotectant for solvent-free cell cryopreservation, but the membrane-impermeability limits its cryoprotective efficiency. Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) membrane perturbation. The self-assembled nanoparticles of the obtained amphiphilic γ-PGA could be adsorbed on the cell membrane by the hydrophobic interaction to disturb the lipid arrangement and increase the membrane permeability of trehalose under hypertonic conditions.

Cryopreservation of human erythrocytes through high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).

Cryopreservation of human erythrocytes suitable cryoprotectants is essential for transfusion during emergencies, but the conventional glycerolization method requires a tedious thawing-deglycerolization process. Alternatively, trehalose, a nonreducing disaccharide, has gained much attention as a biocompatible cryoprotectant due to its nature in living organisms capable of surviving extreme cold and desiccation. In this work, cryopreservation of human erythrocytes was realized through high intracellular trehalose enhanced by benzyl alcohol at 4 °C with membrane stabilization of maltotriose-grafted ε-poly(L-lysine).

[Molecular analysis of 23 cases of B subgroup].

Objective: To explore the molecular reasons of weak expression of B antigen on the red cell.

Methods: Serological test for blood group was carried out, including red cell and plasma grouping, and anti-A1 and anti-H testing, and confirming weak A or B antigens by adsorption and elution. Exons 1-7 were sequenced directly, and one of them was cloned and sequenced.

NonFreezable Preservation of Human Red Blood Cells at -8 °C.

Red blood cell (RBC) preservation is very important in human health. The RBCs are usually preserved at 4 ± 2 °C without freezing or at a very low temperature (-80 °C or liquid nitrogen) with deep freezing. Herein, non freezable preservation of RBCs at a subzero temperature is reported to prolong the preservation time compared with that at 4 ± 2 °C.

Development of Icephilic ACTIVE Glycopeptides for Cryopreservation of Human Erythrocytes.

Ice formation and recrystallization exert severe impairments to cellular cryopreservation. In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes. Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with dsorption on membrane surfaces, ryopreservation with rehalose, and cephilicity for alidity of human rythrocytes.

Characteristics and causes of pre-donation deferrals at a Chinese blood center.

Objectives: The purpose of this retrospective analysis was to understand the causes and characteristics of the pre-donation deferral, so as to enhance blood safety and improve donor satisfaction.

Background: Blood transfusion is crucial in managing a variety of different medical and surgical needs. Its benefits are manifold but there are risks for the recipients.

Luminex xMAP combined with Western blot improves HIV diagnostic sensitivity.

Currently, Western blot is used to confirm the initial serodiagnosis of HIV infection by antibody detection. However, a major deficiency of the Western blot relates to a lack of sufficient sensitivity in detecting HIV antibodies. This report describes a simple, sensitive and inexpensive bead-based assay for detection of early HIV infection.

Evaluation of an individual-donation nucleic acid amplification testing algorithm for detecting hepatitis B virus infection in Chinese blood donors.

Background: This multicenter study was performed to evaluate the efficiency of a multiplex individual-donation nucleic acid amplification technology (ID-NAT) and discriminatory testing algorithm for detecting hepatitis B virus (HBV) infection in Chinese blood donors.

Study Design And Methods: A total of 1,205,796 hepatitis B surface antigen (HBsAg)-nonreactive donations from 10 blood centers were tested by ID-NAT using the Ultrio assay. Multiplex Ultrio-reactive donations were tested in the discriminatory tests as well as in quantitative polymerase chain reaction (qPCR) and in supplemental electrochemiluminescence immunoassays for HBsAg, hepatitis B surface antibody (anti-HBs), hepatitis B e antigen, and antibody to hepatitis B core antigen (anti-HBc).

Identification of the novel allele HLA-A*33:22 by sequence-based typing in a Chinese individual.

The novel A*33:22 differs from A*33:03:01 by two nucleotides at position 12(A>G)and 19(T>G)of exon 3.

A novel allele HLA-B*51:63 was identified by sequence-based typing in a Chinese individual.

The novel B*51:63 is identical to B*51:01:01 with an exception of one base substitution at position 76 (A > C) of exon 3 resulting in codon #116 changed from TAC (Tyr) to TCC (Ser).

A new HLA-B*15 variant, B*15:144, identified by sequence-based typing in a Chinese individual.

A novel human leukocyte antigen-B allele, officially named B*15:144 allele, the previous designation B*9544 allele, was found in a potential Chinese bone marrow donor when direct sequence-based typing was carried out. The novel B*15:144 is identical to B*15:02:01 with the exception of two base substitution at position 195 (C>T), 196 (T>G) of exon 3 resulting in codon #156 changed from CTG (Leu) to TGG (Trp).

Identification of the novel allele HLA-B*4076 by sequence-based typing in a Chinese individual.

A novel human leukocyte antigen-B allele, officially named B*4076 allele, was found in a potential Chinese bone marrow donor when direct sequence-based typing was carried out. The novel B*4076 is identical to B*400101 with an exception of one base substitution at position 239(C>A)of exon 2 resulting in codon #80 changed from AAC (Asn) to AAA (Lys).

Sequence-based typing reveals the novel HLA-DRB1*1467 allele in a Chinese individual.

We report here a novel DRB1*1467 allele identified by sequence-based typing in a Chinese individual. The novel DRB1*1467 is identical to DRB1*140301 with an exception of one base substitution at position 186 (C>A) of exon 2 resulting in codon 67 changed from CTC (Leu) to ATC (Ile).

Identification of a novel HLA-B*54 allele, B*5411, by sequence-based typing in a Chinese individual.

We report here a novel human leukocyte antigen (HLA)-B*5411 allele identified by sequence-based typing in a Chinese individual. The novel B*5411 is identical to B*5404, with the exception of four base substitutions at position 120 (A>C), 134 (G>C), 143 (C>G), and 156 (A>T) of exon 3 resulting in codon no. 131, changed from AGC (Ser) to CGC (Arg), and codon no.

HLA-B*9518 allele identified in the Chinese population.

We report here a novel HLA-B*9518 allele by sequence-based typing in the Chinese population. The new B*9518 is identical to B*150101 with an exception of one base substitution at position 19 of exon 3 where a 'G' change to 'T' resulting in codon 97 changed from AGG (Arg) to ATG (Met).