Significance of plasma D-dimer in relation to the severity of atherosclerosis among patients evaluated by non-invasive indices of cardio-ankle vascular index and carotid intima-media thickness.

The aim of this study is to elucidate the usefulness of plasma D-dimer for the prediction of thrombotic events in highly atherosclerotic patients. The severity of atherosclerosis was measured by non-invasive methods including cardio-ankle vascular index (CAVI) and carotid intima-media thickness (IMT) in 100 patients with atherosclerosis aged 72.1 years on average.

[Significance of the stool occult blood test in patients with thrombotic disease under treatment with low dose aspirin].

We encountered lower gastrointestinal bleeding in 16 patients taking a low dose of aspirin and examined the effect of low aspirin dose on the stool occult blood test in 49 thrombotic patients (mean: 76.7 +/- 9.6 years old) including 39 with cerebral infarction, 8 with ischemic heart disease and 2 with atrial fibrillation.

Urinary protein C inhibitor binding region in the A alpha-chain of human fibrinogen.

This paper describes the binding region of urinary protein C inhibitor (PCI) in the A alpha-chain of human fibrinogen. The A alpha-chain was digested by staphylococcal V8 protease, yielding five main peptides consisting of 10, 11, 11.5, 12 and 14 kDA bands on SDS-PAGE.

Urinary protein C inhibitor binding region in the B beta-chain of human fibrinogen.

The urinary protein C inhibitor (PCI) binding region in the B beta-chain of human fibrinogen was examined by ligand blotting, reverse-phase HPLC and amino acid sequencing. The B beta-chain, isolated from reduced and pyridylethylated fibrinogen, was digested with staphylococcal V8 protease to yield eight peptides consisting of 10, 12, 13, 13.5, 14, 16, 17 and 18 kDa bands and the cleaved peptides were ligand-blotted.

Binding of urinary protein C inhibitor to fibrin(ogen) and its binding mechanism.

The region and mechanism of urinary protein C inhibitor (PCI) binding to fibrin(ogen) were examined using fibrin(ogen)-Sepharose and ligand blotting. Urinary PCI bound to fibrin(ogen)-Sepharose in a heparin-dependent manner at a level about 1.6-fold higher to fibrin-Sepharose than to fibrinogen-Sepharose.

Quantitative assay of the carbohydrate in urokinase-type plasminogen activator by lectin-enzyme immunoassay.

We developed an enzyme immunoassay (EIA) for the measurement of Asn302-linked carbohydrate in urokinase-type plasminogen activator (u-PA) using peroxidase (HRP)-labelled lectins. u-PA antigen in the sample was immunologically bound to microtitre plate wells by anti-u-PA IgG and the binding of HRP-labelled lectins [Con A (Concanavalin A), WGA (wheat germ agglutinin), PNA (peanut agglutinin), CSA (Scotch broom), GS-I (Groffonia simplicifollia) and SBA (soybean agglutinin)] to the carbohydrate of u-PA was measured. The lectin-EIA was dose-dependent in the range 6-6000 IU/ml of u-PA using Con A and WGA.