25 results match your criteria: "Third Medical University[Affiliation]"
Medicine (Baltimore)
January 2023
Department of Gynecology and Obstetrics, The Second Hospital of Dalian Medical University, Dalian, Liaoning, China.
Rationale: Choriocarcinoma is a highly invasive gestational trophoblastic neoplasm, usually metastasis to lung and brain, but occurrence of choriocarcinoma following spontaneous abortion presenting as a vertebral tumor is extremely rare, to the best of our knowledge. Because of the poor diagnosis and high malignancy, the low progression-free survival follows up.
Patient Concerns: We here are reporting a case of choriocarcinoma that presented with vertebral tumor induced paralysis of limbs and incontinence of urine.
J Enzyme Inhib Med Chem
December 2023
Department of Stem Cell and Regenerative Medicine, Southwest Hospital; Third Military Medical University (Army Medical University), Chongqing, China.
High aldehyde dehydrogenase (ALDH) activity is a metabolic feature of adult stem cells and various cancer stem cells (CSCs). The ALDEFLUOR system is currently the most commonly used method for evaluating ALDH enzyme activity in viable cells. This system is applied extensively in the isolation of normal stem cells and CSCs from heterogeneous cell populations.
View Article and Find Full Text PDFChin Neurosurg J
June 2020
Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.
Background: Recent advances in surgical and neuroprotective strategies could effectively manage the pathophysiological progression of subarachnoid hemorrhage (SAH). However, pulmonary dysfunction frequently occurs in SAH patients with an increased risk of unsatisfactory outcomes. Based on the similar microvascular structures in the blood-air barrier and blood-brain barrier and possible brain-lung crosstalks, we believe that pericytes may be involved in both neurological and pulmonary dysfunction after SAH.
View Article and Find Full Text PDFFront Med (Lausanne)
April 2020
Department of Nephrology, Institute of Nephrology of Chongqing and Kidney Center of PLA, Xinqiao Hospital, Army Medical University (Third Medical University), Chongqing, China.
The aim of this study was to identify the predictive role of baseline anti-erythropoietin (anti-EPO) antibody levels in follow-up EPO demand in maintenance dialysis patients with end-stage renal disease (ESRD). Baseline routine blood parameters, clinical data, dialysis-related parameters, EPO, anti-EPO antibody, and anti-EPO-receptor antibody were also measured. Differences in the abovementioned variables were compared among four intervals of the EPO demand index (EDI).
View Article and Find Full Text PDFArch Virol
May 2020
Medical School, Anhui University of Science and Technology, Huainan, 232001, Anhui, People's Republic of China.
To determine the status of human cytomegalovirus (HCMV) infection in human milk in China, a total of 510 human milk samples obtained from three provinces, including 211 donor human milk samples from human milk banks and 299 milk samples obtained from the mothers of premature infants, were tested to detect HCMV DNA. Overall, 46.4% of the donated milk samples and 59.
View Article and Find Full Text PDFJ Neuroinflammation
January 2020
Department of Neurosurgery, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, 400038, China.
Objective: The potential roles and mechanisms of pericytes in maintaining blood-brain barrier (BBB) integrity, which would be helpful for the development of therapeutic strategies for subarachnoid hemorrhage (SAH), remain unclear. We sought to provide evidence on the potential role of pericytes in BBB disruption and possible involvement and mechanism of CypA signaling in both cultured pericytes and SAH models.
Methods: Three hundred fifty-three adult male C57B6J mice weighing 22 to 30 g, 29 CypA mice, 30 CypA (flox/flox) mice, and 30 male neonatal C57B6J mice were used to investigate the time course of CypA expression in pericytes after SAH, the intrinsic function and mechanism of CypA in pericytes, and whether the known receptor CD147 mediates these effects.
Nat Commun
January 2020
Mucosal Immunology Section, National Institute of Dental and Craniofacial Research (NIDCR), National Institutes of Health (NIH), 30 Convent Drive, Bethesda, MD, 20892, USA.
The molecular pathways underlying the development of innate lymphoid cells (ILCs) are mostly unknown. Here we show that TGF-β signaling programs the development of ILC2s from their progenitors. Specifically, the deficiency of TGF-β receptor II in bone marrow progenitors results in inefficient development of ILC2s, but not ILC1s or ILC3s.
View Article and Find Full Text PDFBiochimie
August 2018
Institute of Toxicology, College of Preventive Medicine, The Third Medical University, Chongqing, China; Public Health Laboratory Sciences and Toxicology, West China School of Public Health, Sichuan University, Chengdu, China. Electronic address:
rNTPs are structurally similar to dNTPs but are largely molar excessive in cell than dNTPs. rNTPs are inevitably incorporated into DNA to form rNMPs. Long RNA primers can also be incorporated into lagging-strand DNA.
View Article and Find Full Text PDFJ Matern Fetal Neonatal Med
May 2019
p Department of Obstetrics , Shengjing Hospital of China Medical University, Shenyang , China.
Objective: This study aimed to construct new reference charts and equations for fetal biometry from Chinese fetuses at 15-40 weeks and to compare them with previous references.
Method: This was a prospective, cross-sectional study of singleton pregnancies in patients with a confirmed gestational age who underwent ultrasound examinations between the 15th and 40th weeks of gestation. Each woman was selected only once for this study.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
October 2006
Institute of Burn Research, Southwest Hospital, Third Medical University, State Key Laboratory of Trauma, Burn and Combined Injury, Chongqing 400038, China.
Objective: To observe the effect of glutamine given through different avenues on intestine mucosal barrier damage induced by severe burn injury.
Methods: One hundred and sixty Wistar rats were randomly divided into four groups: namely normal control (C group), burned control (B group), parenteral nutrition with glutamine (PN+GLN group) and enteral nutrition with glutamine (EN+GLN group). Rats in B group, PN+GLN group, and EN+GLN group were subjected to 30% total body surface area (TBSA) full-thickness burn injury.
Zhongguo Fei Ai Za Zhi
April 2006
Department of Oncology, Xinqiao Hospital of the Third Medical University of PLA, Chongqing 400037, P.R.China.
Background: p16INK4a and p14ARF, encoded by gene INK4a/ARF located at chromosome 9p21, are cyclin dependent kinase (CDK) inhibitors. Both p16INK4a and p14ARF are cell cycle regulatory proteins and play an important role in Rb and p53 passways respectively. In this study, wild-type INK4a/ARF gene was transfected into human lung adenocarcinoma cell line A549, in which this gene site was lost, and the effects on the cell's biological behavior were investigated.
View Article and Find Full Text PDFZhongguo Ying Yong Sheng Li Xue Za Zhi
November 2004
Department of Cardiovascular Surgery, Xinqiao Hospital, Third Medical University, Chongqing 400037, China.
Aim: To evaluate whether protein phosphorylation and dephosphorylation in nuclei play roles in the development of myocardial hypertrophy, distribution of protein kinases and phosphatases in cell fractions were determined.
Methods: The model of hypertensive rat was established by abdominal aortic constriction. Velocity and isopyknic gradient centrifugation was employed to fractionate rat myocardium to membrane, cytosol and nuclei.
Shi Yan Sheng Wu Xue Bao
June 2002
Department of Cardiology, Xinqiao hospital, Department of Pathophysiology, Third Medical University, Chongqing 400037.
To investigate the regulation of Ca2+ in the isolated cardiac nuclei from rats which may illuminated the mechanism of nuclear calcium transport system. Elocity and isopyknic gradient centrifugation were employed to fractionate rat cardiac nuclei. Then fluo-4 confocal microscopy techniques was used to verify the changes of nuclear Ca2+.
View Article and Find Full Text PDFZhonghua Gan Zang Bing Za Zhi
May 2004
Institute of Infections Diseases, Southwest Hospital, Third Medical University, Chongqing 400038, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi
March 2000
Institute of Combined Injury, The Third Medical University, Chongqing 400038, China.
Besides hematopoietic stem cells, bone marrow also contains another type of stem cells called mesenchymal stem cells (MSCs). With different induced conditions, MSCs have the ability to differentiate into a variety of nonhematopoietic tissue cells, including osteoblasts, chondroblast, adipocytes, myoblasts, astrocytes, and so on. MSCs can be readily obtained from bone marrow by their adhesion to plastic and expansion in culture.
View Article and Find Full Text PDFZhongguo Zhong Xi Yi Jie He Za Zhi
January 2001
Institute of Internal Medicine of Respiratory Diseases, Xinqiao Hospital, Third Medical University of PLA, Chongqing 400037.
Objective: To observe the effect of Tripterygium wilfordii on Th1, Th2 cytokines in asthma patients for further study on the therapeutic mechanism.
Methods: Twelve patients of middle or severe asthma were treated by Tripterygium polyglucoside 40 mg or 60 mg daily for 4 weeks. Blood of patients was colleted before and after treatment for serum and peripherol blood mononuclear cells (PBMC) preparation.
Zhonghua Yi Xue Za Zhi
April 2002
Department of Cardiology, Nanfang Hospital, Third Medical University, Guangzhou 510515, China.
Objective: To investigate a new alternative splicing isoform of vascular endothelial growth factor (VEGF) gene in human bejings.
Methods: The total RNA of the lung tissue of a legally aborted 4-month-old human fetus was isolated and then amplified by RT-PCR. The amplified product was cloned into the pMD18-T plasmid and pcDNA3.
Chin Med J (Engl)
March 2002
Department of Medical Laboratory, South-Western Hospital, The Third Medical University, Chongqing 400038, China.
Objective: To establish a method for rapid detection and sub-typing of human papilloma virus (HPV).
Methods: We utilized the piezoelectric genosensor (PG) technique, which is a combination of the piezoelectric biosensor and gene chips for HPV identification in 22 recurrent biopsy specimens and 22 corresponding original biopsy specimens. The control samples came from normal tissue of healthy persons.
Sheng Li Xue Bao
August 2001
Department of Cardiology, Xinqiao Hospital, Third Medical University, Chongqing 400037.
In order to clarify the importance of nuclear IP(3)Rs in the development of myocardial hypertrophy, a hypertensive rat model was established by abdominal aortic constriction, and velocity and isopyknic gradient centrifugation was employed to fractionate the cardiac nuclei. The maximal number of binding sites (B(max)) and dissociation constant (Kd) of IP(3) to the nuclear envelopes were measured by [(3)H] IP(3) binding assay. The existence of IP(3)Rs on myocardial nuclear envelope was confirmed.
View Article and Find Full Text PDFCell Res
September 2001
Institute of Immunology, PLA, The Third Medical University, Chongqing, China.
To acquire the phage-displayed mimotopes which mimic the specificity of hepatitis B virus surface antigen (HBsAg), a random peptide library expressing linear peptide with 12 amino acids in length were used to screen with the serum from a hepatitis B virus infected patient in the recovery phase. After 3 rounds of biopanning, the positive phages were confirmed by competitive ELISA using HBsAg/P33. Two phagotopes were identified and one of them was confirmed as mimotope by competition experiment.
View Article and Find Full Text PDFZhonghua Jie He He Hu Xi Za Zhi
March 1998
Cancer Center, Xin Qiao Hospital, Third Medical University of PLA, Chong Qing 400037.
Objective: To study the expression and prognostic significance of cathepsin D(CD) in the patients with lung cancer.
Method: A rapid immunohistochemical method (streptavidin-peroxidase conjugated method, SP) was used to detect CD expression of the NSCLC patients in 64 cases lung cancer tissues and paired lymph nodes samples.
Result: The results showed positive expression rate of CD was 66% (42/64) in the lung cancer tissues, of which squamous cell cancer was 57% (17/30), adenocarcinoma was 74%(25/34).
Surg Radiol Anat
February 1998
Department of Anatomy, Third Medical University, Chongqing, P.R. China.
The hepatic ligamentum teres was investigated in 30 adult specimens. The ligament represents a fibrous remnant of the umbilical v. and a small irregular lumen still exists in the ligament in adult life.
View Article and Find Full Text PDFZhonghua Jie He He Hu Xi Za Zhi
June 1996
Department of Respiration, Dapin Hospital, Third Medical University, Chongqing.
Objective: To observe the dynamic changes of eosinophils (EOS) adhesive ability in the course of eosinophils activation.
Methods: The adhesive ability EOS to vascular endothelial cell (EC) was measured by the use of a microminpulation technique.
Results: The critical separation stress (Sc) between rest EOS and EC was 0.
J Clin Endocrinol Metab
April 1993
Third Medical University Clinic, Vienna, Austria.
Self-administration of very high doses of androgenic anabolic steroids is common use in power athletes because of their favorable effect on performance. Since androgenic steroids decrease serum T4-binding globulin (TBG) concentrations dramatically, we were interested in the effects of this procedure on thyroid function: we performed TRH tests (200 micrograms Relefact, i.v.
View Article and Find Full Text PDFRes Exp Med (Berl)
November 1989
Third Medical University Hospital, Dept. of Internal Medicine, Endocrinology and Metabolism, Mainz, Federal Republic of Germany.
Hypothalamic concentrations of epinephrine and norepinephrine were determined in rats following 6-hydroxydopamine lesions of the locus coeruleus and subcoeruleus system and following sham-operation. These concentrations were correlated with pituitary ACTH content. While the lesion procedure did not have a major effect on hypothalamic monoamine levels, we were able to demonstrate a strong negative correlation between hypothalamic epinephrine and pituitary ACTH content independent of the experimental condition.
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