Beyond Trial Counts: Considerations for Measuring Play and Engagement During Early Intervention for Autistic Children.

Play is critical to child development. In early childhood, object play evolves from exploratory behavior to complex symbolic play. Engagement during play, particularly joint engagement, is essential for learning and social interaction.

Indirect associations between immigration-related stressors and latine adolescents' depressive symptoms: The moderating roles of familism, nativity, and gender.

Objectives: This study examined the indirect relationship between immigration-related stressors and depressive symptoms via family conflict and whether familism values moderated this relationship in a sample of U.S. Latine youth.

An evaluation of the usability and durability of 3D printed versus standard suture materials.

The capability to produce suture material using three-dimensional (3D) printing technology may have applications in remote health facilities where rapid restocking of supplies is not an option. This is a feasibility study evaluating the usability of 3D-printed sutures in the repair of a laceration wound when compared with standard suture material. The 3D-printed suture material was manufactured using a fused deposition modelling 3D printer and nylon 3D printing filament.

A novel device for swift and efficient CD44 protein digestion of pipette tips in human serum.

For molecular diagnostics in modern biomedical research, electrospray ionisation mass spectrometry (ESI-MS) based on proteome profiling is important. Now a days, sample preparation such as proteolysis and protein extraction remain incredibly challenging and inefficient. Recent sample-preparation methods based on micro tips show promising results toward the aim "a proteome in an hour".

Photographs and Parent Training to Support Conversations about Past Events between Caregivers and Children with Autism.

Objectives: Children with autism spectrum disorder experience communication difficulties that can make it challenging to engage in conversations. Their caregivers also often struggle with finding ways to support the child's communication. Parent-implemented interventions and visual supports are evidence-based practices to support the communication skills of children with autism.

Depletion of HIV reservoir by activation of ISR signaling in resting CD4T cells.

HIV reservoirs are extremely stable and pose a tremendous challenge to clear HIV infection. Here, we demonstrate that activation of ISR/ATF4 signaling reverses HIV latency, which also selectively eliminates HIV+ cells in primary CD4T cell model of latency without effect on HIV-negative CD4T cells. The reduction of HIV+ cells is associated with apoptosis enhancement, but surprisingly is largely seen in HIV-infected cells in which gag-pol RNA transcripts are detected in HIV RNA-induced ATF4/IFIT signaling.

A general equilibrium assessment of COVID-19's labor productivity impacts on china's regional economies.

This study introduces a database for analyzing COVID-19's impacts on China's regional economies. This database contains various sectoral and regional economic outcomes at the weekly and monthly level. In the context of a general equilibrium trade model, we first formulate a mathematical representation of the Chinese regional economy and calibrate the model with China's multi-regional input-output table.

How Individuals Use Metaphors to Negotiate Fertility Treatment Decision-Making with Their Romantic Partners.

Fertility problems, or the inability to conceive or carry a pregnancy to term for a period of over 12 months while engaging in unprotected sex, affects 12% of women and 9% of men of childbearing age. To answer calls for more research about individuals' fertility decision-making (DM) with their partners, we conducted in-depth, semi-structured interviews with 53 individuals who have experienced fertility decision-making with a romantic partner at some point in their lives. Our findings indicate at least three primary ways individuals and their partners navigated their decision-making communication in their infertility "journeys:" (1) the , (2) , and (3) approaches.

Interpersonal emotion regulation mitigates the link between trait neuroticism and a more negative valence bias.

Emerging research suggests that trait neuroticism is associated with enhanced attention to and perception of negative emotional stimuli, increasing the risk for multiple forms of psychopathology including depression and anxiety. However, modifiable factors such as certain forms of emotion regulation have the potential to weaken this association. In a large sample ( = 1252), we investigated the link between neuroticism and valence bias in response to stimuli that have the potential for both positive and negative interpretations and examined the moderating role of interpersonal emotion regulation.

Liability in the Time of Coronavirus: The Ethical Necessity of Expanding the Legal Protections Afforded to Healthcare Workers During the COVID-19 Pandemic.

Although discussions have begun regarding the ways in which healthcare providers and individuals in fields adjacent to healthcare might be exposed to legal sanctions involving COVID-19, the complete scope of the legal risks is still largely unknown. This essay explores how current laws in the United States fail to offer adequate protections: (1) to healthcare workers (HCW) practicing under significantly altered standards of care, and (2) to individuals involved in the allocation of scarce resource decision-making process. Using research on Second Victim Syndrome and Medical Malpractice Stress Syndrome, legal protections are presented to provide HCW a form of "moral buffering" to help prevent further traumatizing them for shouldering extraordinary burdens during the COVID-19 pandemic.

Elucidation of dynamic microRNA regulations in cancer progression using integrative machine learning.

Motivation: Empowered by advanced genomics discovery tools, recent biomedical research has produced a massive amount of genomic data on (post-)transcriptional regulations related to transcription factors, microRNAs, long non-coding RNAs, epigenetic modifications and genetic variations. Computational modeling, as an essential research method, has generated promising testable quantitative models that represent complex interplay among different gene regulatory mechanisms based on these data in many biological systems. However, given the dynamic changes of interactome in chaotic systems such as cancers, and the dramatic growth of heterogeneous data on this topic, such promise has encountered unprecedented challenges in terms of model complexity and scalability.

Bicultural stress and internalizing symptoms among U.S. Latinx youth: The moderating role of peer and parent support.

Objective: U.S. Latinx youth are at increased risk for internalizing problems, perhaps due to high levels of bicultural stress.

Self-compassion mediates the link between attachment security and intimate relationship quality for couples navigating pregnancy.

Millions of couples navigate the transition from pregnancy to postpartum in a given year, and this period of change and adjustment in the family is associated with elevated risk for intimate relationship dysfunction. Self-compassion has the potential to promote skills that are essential for healthy adaptation (e.g.

Communication changes the effects of sexual pain on sexual frequency in the pregnancy to postpartum transition.

Purpose: To evaluate how sexual pain influences changes in sexual frequency from the pregnancy to postpartum transition, and to examine how couple's sexual communication interacts with sexual pain during pregnancy.

Methods: We explored data following 159 mixed-sex couples across the transition from pregnancy to 6 months postpartum. Couples completed the Relationship Quality Interview and the Marital Satisfaction Inventory-Revised, which assessed their sexual communication, pain and other sexual problems during pregnancy, and frequency of intercourse at pregnancy and postpartum.

Interparental Relationship Quality During Pregnancy: Implications for Early Parent-Infant Bonding and Infant Socioemotional Development.

Research demonstrates the central role of mother-child relationships in child socioemotional development; however, families are complex systems and the unique roles of multiple family subsystems in early infant development have received less attention. In the present study, we investigated the role of multiple family relationships (interparental, mother-infant, father-infant), during pregnancy and the first month after childbirth, in infant socioemotional functioning at 6 months. We administered semi-structured interviews during pregnancy to assess multiple qualities of the interparental relationship.

Fed-batch polyhydroxybutyrate production by Paraburkholderia sacchari from a ternary mixture of glucose, xylose and arabinose.

Polyhydroxybutyrate (PHB) is a biodegradable bioplastic that is comparable with many petroleum-based plastics in terms of mechanical properties and is highly biocompatible. Lignocellulosic biomass conversion into PHB can increase profit and add sustainability. Glucose, xylose and arabinose are the main monomer sugars derived from upstream lignocellulosic biomass processing.

Ruggedness testing of liquid chromatography-tandem mass spectrometry system components using microbiome-relevant methods and matrices.

Recently, an opportunity to perform a broad ruggedness assessment of our liquid chromatography-tandem mass spectrometry (LC-MS/MS) system presented itself during the analytical planning phase of a large-scale human fecal microbiome study. The specific aim of this project was to study the microbial-mediated metabolism of a targeted set of bile acids/salts by mixed bacterial communities cultured from the feces of 12 healthy volunteers when grown in a custom growth medium and following exposure to different clinically-relevant antibiotics. The magnitude of this study offered a rare opportunity to significantly stress procedures and LC-MS/MS system components comprised in our bile acid/salt targeted metabolomics method.

A high-throughput LC-MS/MS method for the measurement of the bile acid/salt content in microbiome-derived sample sets.

Due to the physicochemical properties of bile acids/salts (., hydrophobic and ionizable), the application of reverse-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods are ideally suited for the measurement of these compounds in a host of microbiologically-relevant matrices. Here, we provide a detailed bioanalytical protocol that contains several modifications of a method previously described by Wegner et al.

Tests in short supply? Try group testing.

and explain how, by pooling specimens, testing capacity for SARS-CoV-2 can be increased.

Human body-fluid proteome: quantitative profiling and computational prediction.

Empowered by the advancement of high-throughput bio technologies, recent research on body-fluid proteomes has led to the discoveries of numerous novel disease biomarkers and therapeutic drugs. In the meantime, a tremendous progress in disclosing the body-fluid proteomes was made, resulting in a collection of over 15 000 different proteins detected in major human body fluids. However, common challenges remain with current proteomics technologies about how to effectively handle the large variety of protein modifications in those fluids.

Fed-batch cultivation and adding supplements to increase yields of polyhydroxybutyrate production by Cupriavidus necator from corn stover alkaline pretreatment liquor.

The aim of this study was to evaluate polyhydroxybutyrate (PHB) production and productivity with supplements under fed-batch cultivation at bioreactor scale (1.3 L). In this study, multiple supplements including oxidative enzyme, mediators, surfactants and silicon nanoparticles were added to Cupriavidus necator culture growing on alkaline pretreatment liquor (APL).

Flow cytometry for quantitation of polyhydroxybutyrate production by Cupriavidus necator using alkaline pretreated liquor from corn stover.

Alkaline pretreated liquor (APL) from lignocellulosic feedstock pretreatment is a lignin-rich stream. Polyhydroxybutyrate (PHB), a biodegradable polymer, has been previously synthesized from APL. It is of interest to monitor PHB production and cell number from APL rapidly for process control.

Copper Changes Intestinal Microbiota of the Cecum and Rectum in Female Mice by 16S rRNA Gene Sequencing.

The aim of the present study was to investigate the effects of high concentrations of copper (Cu) on the cecum and rectum of intestinal microbiota in female mice. Twenty-four Kunming mice were weighed and randomly divided into two groups (n = 12 per group) including the control group and Cu group. Cu group was given drinking water with 5 mg/kg-bw copper chloride (CuCl), while the control group was treated with drinking water without CuCl.