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Novel DNA binding properties of the Mcm10 protein from Saccharomyces cerevisiae.
J Biol Chem
September 2009
Department of Molecular, Microbial, and Structural Biology, The University of Connecticut Medical School, Farmington, Connecticut 06030, USA.
The Mcm10 protein is essential for chromosomal DNA replication in eukaryotic cells. We purified the Saccharomyces cerevisiae Mcm10 (ScMcm10) and characterized its DNA binding properties. Electrophoretic mobility shift assays and surface plasmon resonance analysis showed that ScMcm10 binds stably to both double strand (ds) DNA and single strand (ss) DNA.
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