Uncovering Diversity within the Glomeromycota: Novel Clades, Family Distributions, and Land Use Sensitivity.

Arbuscular mycorrhizal fungi (AMF, phylum Glomeromycota) are essential to plant community diversity and ecosystem functioning. However, increasing human land use represents a major threat to native AMF globally. Characterizing the loss of AMF diversity remains challenging because many taxa are undescribed, resulting in poor documentation of their biogeography and family-level disturbance sensitivity.

EHMT1/2 Inhibition Promotes Regression of Therapy-Resistant Ovarian Cancer Tumors in a CD8 T-cell-Dependent Manner.

Poly ADP-ribose polymerase inhibitors (PARPi) are first-line maintenance therapy for ovarian cancer and an alternative therapy for several other cancer types. However, PARPi-resistance is rising, and there is currently an unmet need to combat PARPi-resistant tumors. Here, we created an immunocompetent, PARPi-resistant mouse model to test the efficacy of combinatory PARPi and euchromatic histone methyltransferase 1/2 inhibitor (EHMTi) in the treatment of PARPi-resistant ovarian cancer.

Combining EHMT and PARP Inhibition: A Strategy to Diminish Therapy-Resistant Ovarian Cancer Tumor Growth while Stimulating Immune Activation.

Despite the success of poly-ADP-ribose polymerase inhibitors (PARPi) in the clinic, high rates of resistance to PARPi presents a challenge in the treatment of ovarian cancer, thus it is imperative to find therapeutic strategies to combat PARPi resistance. Here, we demonstrate that inhibition of epigenetic modifiers euchromatic histone lysine methyltransferases 1/2 (EHMT1/2) reduces the growth of multiple PARPi-resistant ovarian cancer cell lines and tumor growth in a PARPi-resistant mouse model of ovarian cancer. We found that combinatory EHMT and PARP inhibition increases immunostimulatory double-stranded RNA formation and elicits several immune signaling pathways in vitro.

Investigating the role of nicotinic acetylcholine receptors in menthol's effects in mice.

The use of menthol in tobacco products has been linked to an increased likelihood of developing nicotine dependence. The widespread use of menthol can be attributed to its unique sensory characteristics; however, emerging evidence suggests that menthol also alters sensitivity to nicotine through modulation of nicotinic acetylcholine receptors (nAChRs). Nicotinic subunits, such as β2 and α5, are of interest due to their implications in nicotine reward, reinforcement, intake regulation, and aversion.

Quantifying microglial morphology: an insight into function.

Microglia are specialized immune cells unique to the central nervous system (CNS). Microglia have a highly plastic morphology that changes rapidly in response to injury or infection. Qualitative and quantitative measurements of ever-changing microglial morphology are considered a cornerstone of many microglia-centric research studies.

Regulation of microglial responses after pediatric traumatic brain injury: exploring the role of SHIP-1.

Introduction: Severe traumatic brain injury (TBI) is the world's leading cause of permanent neurological disability in children. TBI-induced neurological deficits may be driven by neuroinflammation post-injury. Abnormal activity of SH2 domain-containing inositol 5' phosphatase-1 (SHIP-1) has been associated with dysregulated immunological responses, but the role of SHIP-1 in the brain remains unclear.

Training augmentation using additive sensory noise in a lunar rover navigation task.

Background: The uncertain environments of future space missions means that astronauts will need to acquire new skills rapidly; thus, a non-invasive method to enhance learning of complex tasks is desirable. Stochastic resonance (SR) is a phenomenon where adding noise improves the throughput of a weak signal. SR has been shown to improve perception and cognitive performance in certain individuals.

Combinatory EHMT and PARP inhibition induces an interferon response and a CD8 T cell-dependent tumor regression in PARP inhibitor-resistant models.

Euchromatic histone lysine methyltransferases 1 and 2 (EHMT1/2), which catalyze demethylation of histone H3 lysine 9 (H3K9me2), contribute to tumorigenesis and therapy resistance through unknown mechanisms of action. In ovarian cancer, EHMT1/2 and H3K9me2 are directly linked to acquired resistance to poly-ADP-ribose polymerase (PARP) inhibitors and are correlated with poor clinical outcomes. Using a combination of experimental and bioinformatic analyses in several PARP inhibitor resistant ovarian cancer models, we demonstrate that combinatory inhibition of EHMT and PARP is effective in treating PARP inhibitor resistant ovarian cancers.

The atmospheric X-ray imaging spectrometer (AXIS) instrument: Quantifying energetic particle precipitation through bremsstrahlung X-ray imaging.

The Atmospheric X-ray Imaging Spectrometer (AXIS) described in this work is a compact, wide field-of-view, hard x-ray imager. The AXIS instrument will fly onboard the Atmospheric Effects of Precipitation through Energetic X-rays (AEPEX) 6U CubeSat mission and will measure bremsstrahlung x-ray photons in the 50-240 keV range with cadmium-zinc-telluride (CZT) detectors using coded aperture optics. AXIS will measure photons generated by energetic particle precipitation for the purpose of determining the spatial scales of precipitation and estimating electron precipitation characteristics.

Overcoming Isolation: Teenagers' Connectedness to Others During the COVID-19 Pandemic.

The COVID-19 pandemic was characterized by loneliness, especially among teenagers. This study explored the coping mechanisms, activities, challenges, places visited, and sources of support that predicted teenagers feeling connected to others during COVID-19. Data come from surveys administered in the United States and Canada in summer 2022.

Childhood trauma and other formative life experiences predict environmental engagement.

Environmental problems continue to intensify. Yet, despite scientific consensus on threats such as climate change, broadscale public engagement with the issue is elusive. In this paper, we focus on childhood formative experiences and the extent to which they are correlated with environmental engagement.

Task-Dependent Modulation of Auditory Feedback Control of Vocal Intensity.

Auditory feedback control of fundamental frequency (f) is modulated in a task-dependent manner. When voice pitch auditory feedback perturbations are applied in sentence versus sustained-vowel production, larger and faster vocal f responses are measured in sentence production. This task-dependency reflects the scaling of auditory targets for pitch for the precision required in each speech task.

ATF6-Mediated Signaling Contributes to PARP Inhibitor Resistance in Ovarian Cancer.

Unlabelled: High-grade serous ovarian cancer (HGSOC) is the deadliest ovarian cancer histotype due in-part to the lack of therapeutic options for chemotherapy-resistant disease. PARP inhibitors (PARPi) represent a targeted treatment. However, PARPi resistance is becoming a significant clinical challenge.

Synchronization in electric power networks with inherent heterogeneity up to 100% inverter-based renewable generation.

The synchronized operation of power generators is the foundation of electric power network stability and a key to the prevention of undesired power outages and blackouts. Here, we derive the conditions that guarantee synchronization in power networks with inherent generator heterogeneity when subjected to small perturbations, and perform a parametric sensitivity analysis to understand synchronization with varied types of generators. As inverter-based resources, which are the primary interfacing technology for many renewable sources of energy, have supplanted synchronous generators in ever growing numbers, the center of attention on associated integration challenges have resided primarily on the role of declining system inertia.

Patched Local Lunar Gravity Solutions Using GRAIL Data.

We present a method to determine local gravity fields for the Moon using Gravity Recovery and Interior Laboratory (GRAIL) data. We express gravity as gridded gravity anomalies on a sphere, and we estimate adjustments to a background global start model expressed in spherical harmonics. We processed GRAIL Ka-band range-rate data with a short-arc approach, using only data over the area of interest.

What Does It Mean to Heal From Historical Trauma?

Native American peoples' health is impacted by structural legacies of settler colonialism, including land dispossession, racism, and poverty. Responding with care to individuals and communities experiencing past and present traumatic stress from genocide and deeply entrenched structural violence means navigating ongoing grief, restoring self-community and human-ecological relationships, and generating cultural vibrancy.

The influence of a 16-week exercise program, APOE status, and age on executive function task performance: A randomized trial.

Previous research has shown beneficial cognitive changes following exercise training in older adults. However, the work on the potential moderating effects of Apoliprotein E (APOE) ε4 carrier status has been mixed, and the role of exercise intensity remains largely unexplored. The present study sought to examine the influence of APOE ε4 status and exercise intensity on measures of cognitive performance in a group of older adults.

Single-Flux-Quantum Multiplier Circuits for Synthesizing Gigahertz Waveforms With Quantum-Based Accuracy.

We designed, simulated, and experimentally demonstrated components for a microwave-frequency digital-to-analog converter based on single flux quantum (SFQ) circuits and an amplifier based on superconducting-quantum-interference-device (SQUID) stacks. These are key components for a self-calibrated programmable waveform reference for communications metrology capable of synthesizing high-frequency signals with quantum-based output accuracy. The amplifier is an SFQ voltage multiplier circuit that consists of a network of SFQ-splitters and SQUID transformers that provides output signals consisting of quantized pulses.

Potential for Electric Vehicle Adoption to Mitigate Extreme Air Quality Events in China.

Electric vehicle (EV) adoption promises potential air pollutant and greenhouse gas (GHG) reduction co-benefits. As such, China has aggressively incentivized EV adoption, however much remains unknown with regard to EVs' mitigation potential, including optimal vehicle type prioritization, power generation contingencies, effects of Clean Air regulations, and the ability of EVs to reduce acute impacts of extreme air quality events. Here, we present a suite of scenarios with a chemistry transport model that assess the potential co-benefits of EVs during an extreme winter air quality event.

Case studies in physiology: Impact of a long-distance hike on the Pacific Crest Trail on arterial function and body composition in a highly fit young male.

The Pacific Crest Trail (PCT) is a 4265-km hiking trail that extends from the US-Mexican border to the US-Canadian border through the mountain ranges of western North America. Individuals who hike the entire length of the trail in one season (4-6 months) perform long daily exercise durations while exposed to extreme environmental temperatures, high altitudes, intense solar radiation, and the consumption of calorie-rich, nutrient-poor diets. This case study reports changes in arterial function and body composition in a subject before and after a 112-day long-distance hike of the PCT.

Ricochets on Asteroids: Experimental study of low velocity grazing impacts into granular media.

Spin off events and impacts can eject boulders from an asteroid surface and rubble pile asteroids can accumulate from debris following a collision between large asteroids. These processes produce a population of gravitational bound objects in orbit that can impact an asteroid surface at low velocity and with a distribution of impact angles. We present laboratory experiments of low velocity spherical projectiles into a fine granular medium, sand.