Optimization of Enzymatic Deproteination of Northern Shrimp () Shell Chitin Using Commercial Proteases.

Shrimp shells are a key source of chitin, commonly extracted through chemical methods, which may cause minor molecular damage. Nowadays, there is great interest in achieving close to zero protein content in crude chitin in order to use it for high-end markets. Therefore, this study optimized the enzymatic deproteination using two commercial proteases (SEB Pro FL100 and Sea-B Zyme L200) for effective and fast removal of residual protein from Northern shrimp () shell chitin for the first time.

The "What" and "How" of Pantomime Actions.

Pantomimes are human actions that simulate ideas, objects, and events, commonly used in conversation, performance art, and gesture-based interfaces for computing and controlling robots. Yet, their underlying neurocognitive mechanisms are not well understood. In this review, we examine pantomimes through two parallel lines of research: (1) the two visual systems (TVS) framework for visually guided action, and (2) the neuropsychological literature on limb apraxia.

Elemental mapping in single-particle reconstructions by reconstructed electron energy-loss analysis.

For macromolecular structures determined by cryogenic electron microscopy, no technique currently exists for mapping elements to defined locations, leading to errors in the assignment of metals and other ions, cofactors, substrates, inhibitors and lipids that play essential roles in activity and regulation. Elemental mapping in the electron microscope is well established for dose-tolerant samples but is challenging for biological samples, especially in a cryo-preserved state. Here we combine electron energy-loss spectroscopy with single-particle image processing to allow elemental mapping in cryo-preserved macromolecular complexes.

Mexican Hispanics show significant improvement in lung function approximately 1 year after having severe COVID-19.

The long-term effects of COVID-19 on lung function are not understood, especially for periods extending beyond 1 year after infection. This observational, longitudinal study investigated lung function in Mexican Hispanics who experienced severe COVID-19, focusing on how the length of recovery affects lung function improvements. At a specialized COVID-19 follow-up clinic in Yucatan, Mexico, lung function and symptoms were assessed in patients who had recovered from severe COVID-19.

Developing a streamlined risk-adjusted cesarean section rate model for evaluation of obstetrical quality across hospitals by using EHRs: A provincial-scale multicenter retrospective study.

Objective: This study aims to explore a streamlined risk-adjusted cesarean section rate (RCSR) model and to compare its practical application effects with the traditional RCSR models.

Methods: Utilizing obstetric electronic health record (EHR) data from provincial multicenter hospitals, this study establishes a streamlined RCSR model alongside the traditional RCSR model and evaluates the efficacy of both models. Subsequently, the RCSRs of 56 hospitals within the province are calculated and ranked using both models.

Seasonal stability of the rumen microbiome contributes to the adaptation patterns to extreme environmental conditions in grazing yak and cattle.

Background: The rumen microbiome plays an essential role in maintaining ruminants' growth and performance even under extreme environmental conditions, however, which factors influence rumen microbiome stability when ruminants are reared in such habitats throughout the year is unclear. Hence, the rumen microbiome of yak (less domesticated) and cattle (domesticated) reared on the Qinghai-Tibetan Plateau through the year were assessed to evaluate temporal changes in their composition, function, and stability.

Results: Rumen fermentation characteristics and pH significantly shifted across seasons in both cattle and yak, but the patterns differed between the two ruminant species.

Trade-offs between receptor modification and fitness drive host-bacteriophage co-evolution leading to phage extinction or co-existence.

Parasite-host co-evolution results in population extinction or co-existence, yet the factors driving these distinct outcomes remain elusive. In this study, Salmonella strains were individually co-evolved with the lytic phage SF1 for 30 days, resulting in phage extinction or co-existence. We conducted a systematic investigation into the phenotypic and genetic dynamics of evolved host cells and phages to elucidate the evolutionary mechanisms.

Reversible Nucleophilic Ring-Opening of Tetraoxapentacene Derivatives: Accessing New Materials for Thermally Activated Delayed Fluorescence.

We report the unexpected nucleophilic ring-opening reaction of electron deficient dioxins in the presence of carbazole under basic conditions. This nucleophilic ring-opening reaction is reversible under basic conditions in the absence of nucleophiles. Further, we demonstrate that this unexpected reactivity can be used to prepare novel donor-acceptor compounds that are emissive in solution and as thin films and exhibit thermally activated delayed fluorescence (TADF).

Cross-Linguistic Phonetic Variation in Bilingual Speech: Cantonese /n/ > [l] Merger in Early Cantonese-English Bilinguals.

/n/ is merging with /l/ in Cantonese, as well as in several other Chinese languages. The Cantonese merger appears categorical, with /n/ becoming /l/ syllable-initially. This project aims to describe /n/ and /l/ in Cantonese and English speech from early Cantonese-English bilinguals to better understand the status of the merger in Cantonese and its potential for cross-linguistic mutual influence.

"I Needed to be That Voice": A Multi-Party Study of the Healthcare and Social Service Experiences and Needs of Transgender and Gender-diverse Older Adults in Canada.

The social contexts of transgender and gender-diverse (TGD) older adults remain under-examined. In this qualitative study, which involved six virtual focus groups with a total of 21 participants inclusive of TGD adults ages 50+, service providers, and community advocates, we sought to examine the healthcare and social service experiences and needs of TGD older adults in Canada. Drawing theoretically on critical gerontology and intersectionality, and methodologically on interpretive description, we examined the perspectives of different participant groups to develop insight into TGD older adults' issues and priorities in the context of their engagements with systems of care.

The Future is Bright: The Emergence of Glassy Organic Dots for Biological Applications.

Glassy organic dots (g-Odots) are an emerging class of luminescent nanoparticles that offer enhanced photostability, superior brightness, and modular tunability compared to other commonly employed nanoparticles. In the last several years, they have been used as bioimaging probes for single- and multi-photon cellular imaging, exhibiting low cytotoxicity even after several days. While they are emerging as promising materials for use in biological applications, g-Odots face several key challenges before their use can become widespread.

Protocol for exploring the relationship between sound localization and the representation of the body in space.

The influence of multisensory integration on spatial hearing has received more attention in recent years. Notably, incongruent sensory inputs can bias auditory spatial processing. Here, we present a protocol for producing an illusory shift in the localization of a sound source by inducing an unconscious shift in the representation of the body in space.

120 GOPS Photonic tensor core in thin-film lithium niobate for inference and in situ training.

Photonics offers a transformative approach to artificial intelligence (AI) and neuromorphic computing by enabling low-latency, high-speed, and energy-efficient computations. However, conventional photonic tensor cores face significant challenges in constructing large-scale photonic neuromorphic networks. Here, we propose a fully integrated photonic tensor core, consisting of only two thin-film lithium niobate (TFLN) modulators, a III-V laser, and a charge-integration photoreceiver.

Exploring factors affecting adherence to multiple micronutrient supplementation during pregnancy in Cambodia: A qualitative analysis.

For decades, iron-folic acid (IFA) supplements have been provided to pregnant women in Cambodia through antenatal care (ANC) services. However, mounting evidence suggests that multiple micronutrient supplements (MMS) are superior to IFA supplements in achieving positive pregnancy outcomes. The possibility of transitioning from IFA supplements to MMS in government-run health centres is currently being assessed in Cambodia.

Mitigating Grand Challenges in Life Cycle Inventory Modeling through the Applications of Large Language Models.

The accuracy of life cycle assessment (LCA) studies is often questioned due to the two grand challenges of life cycle inventory (LCI) modeling: (1) missing foreground flow data and (2) inconsistency in background data matching. Traditional mechanistic methods (e.g.

Smooth multi-period forecasting with application to prediction of COVID-19 cases.

Forecasting methodologies have always attracted a lot of attention and have become an especially hot topic since the beginning of the COVID-19 pandemic. In this paper we consider the problem of multi-period forecasting that aims to predict several horizons at once. We propose a novel approach that forces the prediction to be "smooth" across horizons and apply it to two tasks: point estimation via regression and interval prediction via quantile regression.

Turning up the heat: infrared sensing during mosquito host-seeking.

The yellow fever mosquito Aedes aegypti uses multiple sensory systems to identify a blood-meal victim. Recent work by Chandel et al. identifies infrared radiation as a medium-distance cue acting through TrpA1 and two opsins to help target hosts, furthering our understanding of the multisensory hunting strategy used by mosquitoes on the prowl.

Emerging lessons from experiences at transitions in care among hospitalised patients with cancer with postdischarge frequent emergency department use: a qualitative study using linked clinical and patient-reported interview data from Quebec, Canada.

Background: While teamwork is essential to providing high-quality patient-centred care, challenges in interprofessional collaboration and decision-making in hospital settings are common, especially for patients with cancer. The purpose of this qualitative study was to identify emerging themes and potential challenges related to hospital discharge experiences among patients hospitalised for cancer who became frequent emergency department (ED) users postdischarge.

Methods: A cohort of patients with cancer discharged from an academic health centre in Montreal (Canada) between October 2014 and November 2016 was assembled.

Climate change undermines seafood micronutrient supply from wild-capture fisheries in Southeast Asia and Pacific Island countries.

Marine ecosystem functions are affected by climate change impacts such as ocean warming, deoxygenation and acidification. These impacts drive changes in distributions and body size of fish species and directly affect fisheries. Wild-capture fisheries are crucial for providing nutrients, livelihoods, and employment in tropical Southeast Asia and Pacific Island countries, where coastal communities are highly vulnerable to climate change.

Three-dimensional vegetation structure drives patterns of seed dispersal by African hornbills.

Three-dimensional (3D) vegetation structure influences animal movements and, consequently, ecosystem functions. Animals disperse the seeds of 60%-90% of trees in tropical rainforests, which are among the most structurally complex ecosystems on Earth. Here, we investigated how 3D rainforest structure influences the movements of large, frugivorous birds and resulting spatial patterns of seed dispersal.

Salvia miltiorrhiza in osteoporosis: a review of its phytochemistry, traditional clinical uses and preclinical studies (2014-2024).

Osteoporosis becomes a global public health concern due to its rising prevalence and substantial impact on life quality. Salvia miltiorrhiza Bunge (Salviae Miltiorrhizae Radix et Rhizoma, SM) has been firstly recorded in Shen Nong's Herbal Classic, and is frequently prescribed in conjunction with other herbs for the management of osteoporosis. This systematic review aims to comprehensively analyze the recent advances of SM on osteoporosis in traditional Chinese clinical uses and preclinical investigations.