A novel tape-free sample preparation method for human osteochondral cryosections for high throughput hyperspectral imaging.

Understanding the osteochondral junction, where non-mineralised cartilage and mineralised bone converge, is crucial for joint health. Current sample preparation techniques are insufficient for detailed spatial hyperspectral imaging analysis. Using the enhanced Kawamoto method, we used the super cryo embedding medium's temperature-dependent properties to transfer high-quality tissue samples onto slides for spatial imaging analysis.

Intestinal mucosal immunity and type 1 diabetes: Non-negligible communication between gut and pancreas.

Type 1 diabetes (T1D) is a chronic autoimmune disease characterized by T cell-mediated pancreatic β cell loss, resulting in lifelong absolute insulin deficiency and hyperglycaemia. Environmental factors are recognized as a key contributor to the development of T1D, with the gut serving as a primary interface for environmental stimuli. Recent studies have revealed that the alterations in the intestinal microenvironment profoundly affect host immune responses, contributing to the aetiology and pathogenesis of T1D.

'A level-breaking adventure game': A grounded theory study of postgraduate nurses' academic growth trajectory.

Objective: This study aims to explore the key experiences in the academic growth trajectory of postgraduate nurses.

Design And Methods: A qualitative study was designed using Charmaz's constructivist grounded theory. Semi-structured in-depth interviews were conducted, with all sessions being recorded and transcribed verbatim.

Impact of live microbe intake on cardiovascular disease and mortality in adults with diabetes: A nationwide cohort study.

Objective: This study examines the association between dietary intake of live microbes (LM) and the risk of cardiovascular disease (CVD) and cardiovascular mortality in adults with diabetes.

Methods: A retrospective cohort study was conducted using National Health and Nutrition Examination Survey (NHANES) data from 2001 to 2010, with follow-up mortality data through December 31, 2019. A total of 3,955 adults with diabetes were included.

Crosstalk of pyroptosis and cytokine in the tumor microenvironment: from mechanisms to clinical implication.

In the realm of cancer research, the tumor microenvironment (TME) plays a crucial role in tumor initiation and progression, shaped by complex interactions between cancer cells and surrounding non-cancerous cells. Cytokines, as essential immunomodulatory agents, are secreted by various cellular constituents within the TME, including immune cells, cancer-associated fibroblasts, and cancer cells themselves. These cytokines facilitate intricate communication networks that significantly influence tumor initiation, progression, metastasis, and immune suppression.

Multi-omics landscape of childhood simple obesity: novel insights into pathogenesis and biomarkers discovery.

Background: The increasing incidence of childhood obesity annually has led to a surge in physical and mental health risks, making it a significant global public health concern. This study aimed to discover novel biomarkers of childhood simple obesity through integrative multi-omics analysis, uncovering their potential connections and providing fresh research directions for the complex pathogenesis and treatment strategies.

Methods: Transcriptome, untargeted metabolome, and 16 S rDNA sequencing were conducted on subjects to examine transcripts, metabolites in blood, and gut microflora in stool.

The relationship between work-family conflict, stress and depression among Chinese correctional officers: a mediation and network analysis study.

Background: Numerous studies have found that depression is prevalent among correctional officers (COs), which may be related to the work-family conflict (WFC) faced by this cohort. Role conflict theory posits that WFC emerges from the incompatibility between the demands of work and family roles, which induces stress and, in turn, results in emotional problems. Thus, this study seeks to investigate the association between WFC and depression, along with examining the mediating role of stress.

Unlocking the full potential of memory T cells in adoptive T cell therapy for hematologic malignancies.

In recent years, immune cell therapy, particularly adoptive cell therapy (ACT), has shown superior therapeutic effects on hematologic malignancies. However, a challenge lies in ensuring that genetically engineered specific T cells maintain lasting anti-tumor effects within the host. The enduring success of ACT therapy hinges on the persistence of memory T (T) cells, a diverse cell subset crucial for tumor immune response and immune memory upkeep.

Unraveling the Diffusion MRI-Based Glymphatic System Alterations in Children with Rolandic Epilepsy.

Rationale And Objectives: Although dysfunction of the glymphatic system in adult epilepsy has been extensively studied, there is a lack of research on the changes in this system during childhood development, particularly in children with Rolandic epilepsy (RE). This study aimed to investigate the changes in diffusion MRI measures related to the glymphatic function in children with RE.

Materials And Methods: A total of thirty-eight children with RE and thirty-six demographically matched healthy children were enrolled in the study.

Pan-cancer analysis identifies the oncogenic role of in human cancers.

Objective: To investigate expression, prognosis, immune cell infiltration of C () in cancer.

Methods: We used TIMER and GEPIA datasets to analyze the differential expression of in multiple tumors. GEPIA and Kaplan-Meier plotter databases were utilized to observe the prognostic significance of in cancer.

Shared differential factors underlying individual spontaneous neural activity abnormalities in major depressive disorder.

Background: In contemporary neuroimaging studies, it has been observed that patients with major depressive disorder (MDD) exhibit aberrant spontaneous neural activity, commonly quantified through the amplitude of low-frequency fluctuations (ALFF). However, the substantial individual heterogeneity among patients poses a challenge to reaching a unified conclusion.

Methods: To address this variability, our study adopts a novel framework to parse individualized ALFF abnormalities.

Investigating the shared genetic architecture of osteoarthritis and frailty: a genome-wide cross-trait analysis.

Observational studies suggest a link between osteoarthritis (OA) and frailty, but the shared genetic architecture and causal relationships remain unclear. We analyzed X-ray and F-FDG PET/CT images in frail and non-frail individuals and conducted genetic correlation analyses using Linkage Disequilibrium Score Regression (LDSC) based on recent Genome-Wide Association Studies (GWAS) for OA and frailty. We identified pleiotropic single-nucleotide polymorphisms (SNPs) through Cross-Phenotype Association (CPASSOC) and Colocalization (COLOC) analyses and investigated genetic overlaps using Multi-marker Analysis of GenoMic Annotation (MAGMA).

DsbA-L activates TGF-β1/SMAD3 signaling and M2 macrophage polarization by stimulating AKT1 and NLRP3 to promote pulmonary fibrosis.

Background: Pulmonary fibrosis (PF) is a progressive and difficult-to-heal lung disease that poses a significant threat to human life and health. This study aimed to investigate the potential pathological mechanisms of PF and to identify new avenues for the treatment of PF.

Methods: Clinical samples were collected to assess the effect of disulfide-bond A oxidoreductase-like protein (DsbA-L) on PF.

Polydopamine(PDA)-coated diselenide-bridged mesoporous silica-based nanoplatform for neuroprotection by reducing oxidative stress and targeting neuroinflammation in intracerebral hemorrhage.

Oxidative stress (OS) and neuroinflammation are critical pathological processes in secondary brain injury (SBI) after intracerebral hemorrhage(ICH), and their intimate interactions initiate and aggravate brain damage. Thus, targeting oxidative stress and neuroinflammation could be a promising therapeutic strategy for ICH treatment. Here, we report a high-performance platform using polydopamine (PDA)-coated diselenide bridged mesoporous silica nanoparticle (PDA-DSeMSN) as a smart ROS scavenger and ROS-responsive drug delivery system.

Exploring the association between childhood trauma and limbic system subregion volumes in healthy individuals: a neuroimaging study.

Background: Childhood trauma (CT) is a major risk factor for psychiatric disorders. Emotional and cognitive functions are often affected in many psychiatric conditions, and these functions are mediated by the limbic system. However, previous research has primarily focused on patient populations.

Economic Evaluation of Adding Daratumumab to Carfilzomib and Dexamethasone for Relapsed or Refractory Multiple Myeloma.

Objective: To assess the cost-effectiveness of adding daratumumab to carfilzomib and dexamethasone (KdD) in patients with relapsed or refractory multiple myeloma (RRMM).

Materials And Methods: A Markov model was established to estimate health and economic outcomes of carfilzomib and dexamethasone (Kd) with or without daratumumab for RRMM patients over a lifetime horizon. The patients and intervention of the two arms were modeled according to the CANDOR trial.