261 results match your criteria: "The Second Affiliated Hospital of Southern University of Science and Technology[Affiliation]"

A bacterial methyltransferase that initiates biotin synthesis, an attractive anti-ESKAPE druggable pathway.

Sci Adv

December 2024

Key Laboratory of Multiple Organ Failure (Ministry of Education), and Departments of Microbiology and General Intensive Care Unit of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.

The covalently attached cofactor biotin plays pivotal roles in central metabolism. The top-priority ESKAPE-type pathogens, and , constitute a public health challenge of global concern. Despite the fact that the late step of biotin synthesis is a validated anti-ESKAPE drug target, the primary stage remains fragmentarily understood.

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In the context of the global damage caused by coronavirus disease 2019 (COVID-19) and the emergence of the monkeypox virus (MPXV) outbreak as a public health emergency of international concern, research into methods that can rapidly test potential therapeutics during an outbreak of a new infectious disease is urgently needed. Computational drug discovery is an effective way to solve such problems. The existence of various large open databases has mitigated the time and resource consumption of traditional drug development and improved the speed of drug discovery.

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Systemic and dynamic immune landscape of Omicron-infected subjects treated with Lianhua Qingwen capsules.

Acta Pharm Sin B

November 2024

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Collaborative Innovation Center of Hematology, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

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Article Synopsis
  • Radiomics has been used in oncology since 2012 to analyze digital images and convert them into detailed data for evaluating lymph node status; this study reviews literature in the field from 2012 to 2023.
  • A total of 898 articles were analyzed, showing a substantial growth rate of 100.77% in publications, primarily from China, with Fudan University being the top contributor.
  • The research focus has shifted from cancer diagnosis to deep learning applications for predicting lymph node metastasis, indicating an emerging trend of integrating artificial intelligence in these studies.
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Protective Impact of Influenza Vaccination on Healthcare Workers.

Vaccines (Basel)

October 2024

Department of Preventive Medicine and Healthcare-Associated Infection Management, National Clinical Research Center for Infectious Diseases, Third People's Hospital of Shenzhen and the Second Affiliated Hospital of Southern University of Science and Technology, No 29 Bulan Road, Longgang District, Shenzhen 518112, China.

Background: Influenza vaccine uptake among healthcare workers is crucial for preventing influenza infections, yet its effectiveness needs further investigation.

Objectives: This prospective observational study aimed to assess the protective effect of influenza vaccination among healthcare workers in Shenzhen.

Methods: We enrolled 100 participants, with 50 receiving the 2023-2024 quadrivalent influenza vaccine (QIV) and 50 serving as unvaccinated controls.

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[Clinical application of targeted next generation sequencing in detecting rifampicin and rifabutin resistance in tuberculosis patients].

Zhonghua Yu Fang Yi Xue Za Zhi

November 2024

Department of Clinical Laboratory, Shenzhen Third People's Hospital, The Second Affiliated Hospital of Southern University of Science and Technology, National Clinical Research Center for Infectious Diseases, Shenzhen518112, China.

Article Synopsis
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Sequential Immune Acquisition of Monoclonal Antibodies Enhances Phagocytosis of by Recognizing ATP Synthase.

Vaccines (Basel)

September 2024

BSL-3 Laboratory (Guangdong), Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, China.

: The aim of this study was to prepare monoclonal antibodies (mAbs) that broadly target and protect against infection by multi-drug-resistant (MDR) from different sources. : mAb 8E6 and mAb 1B5 were prepared by sequentially immunizing mice with a sublethal inoculation of three heterogeneous serotypes of pan-drug-resistant (PDR) , ST-208, ST-195, and ST-229. : The cross-recognition of heterogeneous bacteria (n = 13) by two mAbs and potential targets was verified, and the in vitro antibacterial efficacy of mAbs was assessed.

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Article Synopsis
  • - Respiratory infectious diseases pose significant public health risks, with heightened focus on prevention and control systems globally due to the COVID-19 pandemic's impact.
  • - The study highlights changes in the epidemiology of respiratory diseases and reports a delayed influenza season in 2023, with A(H1N1) pdm09 as the dominant strain in China.
  • - China aims to enhance its infectious disease control system by developing an intelligent surveillance and early warning system to better manage future health emergencies and optimize resource allocation.
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Reduced B cell frequencies in cord blood of HIV-exposed uninfected infants: an immunological and transcriptomic analysis.

Front Immunol

September 2024

Department of Infection Immunology, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Article Synopsis
  • HEU infants, who are exposed to HIV but uninfected, show abnormal immune functions and higher illness rates compared to HUU infants; the underlying mechanisms remain unclear.
  • The study used flow cytometry and RNA-seq to analyze immune profiles in the cord blood of HEU and HUU infants, comparing them to maternal blood for unique HIV/ART effects.
  • Findings revealed a significant decrease in B lymphocyte levels and a distinct 4-gene signature in HEU infants, suggesting this signature could help identify immune issues in these infants.
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scBubbletree: computational approach for visualization of single cell RNA-seq data.

BMC Bioinformatics

September 2024

Bioinformatics and Computational Biophysics, Faculty of Biology and Centre for Medical Biotechnology (ZMB), University of Duisburg-Essen, 45141, Essen, Germany.

Background: Visualization approaches transform high-dimensional data from single cell RNA sequencing (scRNA-seq) experiments into two-dimensional plots that are used for analysis of cell relationships, and as a means of reporting biological insights. Yet, many standard approaches generate visuals that suffer from overplotting, lack of quantitative information, and distort global and local properties of biological patterns relative to the original high-dimensional space.

Results: We present scBubbletree, a new, scalable method for visualization of scRNA-seq data.

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An IGHG1 variant exhibits polarized prevalence and confers enhanced IgG1 antibody responses against life-threatening organisms.

Nat Immunol

October 2024

School of Life Sciences, Institute for Immunology, State Key Laboratory of Membrane Biology, China Ministry of Education Key Laboratory of Protein Sciences, Beijing Key Lab for Immunological Research on Chronic Diseases, Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing, China.

Evolutionary pressures sculpt population genetics, whereas immune adaptation fortifies humans against life-threatening organisms. How the evolution of selective genetic variation in adaptive immune receptors orchestrates the adaptation of human populations to contextual perturbations remains elusive. Here, we show that the G396R coding variant within the human immunoglobulin G1 (IgG1) heavy chain presents a concentrated prevalence in Southeast Asian populations.

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Hfq-binding small RNA PqsS regulates Pseudomonas aeruginosa pqs quorum sensing system and virulence.

NPJ Biofilms Microbiomes

September 2024

Shenzhen National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen, China.

Pseudomonas aeruginosa is a widespread nosocomial pathogen with a significant to cause both severe planktonic acute and biofilm-related chronic infections. Small RNAs (sRNAs) are noncoding regulatory molecules that are stabilized by the RNA chaperone Hfq to trigger various virulence-related signaling pathways. Here, we identified an Hfq-binding sRNA in P.

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Population Pharmacokinetic Modeling of Pyrazinamide Among Chinese Patients With Drug-Sensitive or Multidrug-Resistant Tuberculosis.

Ther Drug Monit

September 2024

Department of Clinical Laboratory, Shenzhen Third People's Hospital, National Clinical Research Center for Infectious Diseases, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen, Guangdong Province, China; and.

Article Synopsis
  • Pyrazinamide is used to treat both drug-susceptible and multidrug-resistant tuberculosis, and this study looks at factors affecting its pharmacokinetics to improve dosing strategies.
  • A population pharmacokinetic model was developed using data from 222 patients in China, revealing that total body weight significantly impacts the drug's clearance.
  • Recommendations for fixed dosages based on patient weight were established, suggesting doses of 1500 mg for those weighing 40-70 kg, 1750 mg for 70-80 kg, and 2000 mg for 80-90 kg to ensure effective treatment outcomes.
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The bacterial membrane vesicles (MVs) are non-replicative, nanoscale structures that carry specific cargos and play multiple roles in microbe-host interactions. An appropriate MV isolation method that mimics complex pathogen infections in vivo is needed. After bacterial MVs extraction, flagella or pili can be frequently observed along with MVs by transmission electron microscope (TEM).

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Background: Studies investigating the relationship between flavonoid intake and chronic kidney disease (CKD) are limited. This study investigated the association between daily flavonoid intake and CKD in US adults by using data for 2007-2008, 2009-2010, and 2017-2018 from the National Health and Nutrition Examination Survey (NHANES) database.

Methods: This study employed a cross-sectional design and used data from three cycles of the continuous NHANES: 2007-2008, 2009-2010, and 2017-2018.

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Global temporal trends and projections of acute hepatitis E incidence among women of childbearing age: Age-period-cohort analysis 2021.

J Infect

October 2024

Department of Liver Diseases, The Third People's Hospital of Shenzhen, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen, Guangdong 518100, China; National Clinical Research Center for Infectious Diseases, Shenzhen, Guangdong 518100, China. Electronic address:

Background & Aims: Acute hepatitis E (AHE) poses a significant threat to global public health, particularly among women of childbearing age (WCBA), who are at heightened risk for severe pregnancy-related complications. This study aimed to delineate the temporal trends and project future incidence of AHE in WCBA, providing insights crucial for targeted prevention and control strategies.

Methods: Data on AHE incidence from the Global Health data 2021.

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Development of a dual-component biosensor for rapid and sensitive detection of influenza H7 and H5 subtypes.

Talanta

December 2024

Guangdong Provincial Key Laboratory of New Drug Screening, NMPA Key Laboratory for Research and Evaluation of Drug Metabolism and Guangdong-Hong Kong-Macao Joint Laboratory for New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China; Key Laboratory of Infectious Diseases Research in South China, Ministry of Education, Southern Medical University, Guangzhou, 510515, China. Electronic address:

The outbreak of highly pathogenic influenza virus subtypes, such as H7 and H5, presents a significant global health challenge, necessitating the development of rapid and sensitive diagnostic methods. In this study, we have developed a novel dual-component biosensor assembly, each component of which incorporates an antibody fused with a nano-luciferase subunit. Our results demonstrate the effectiveness of this biosensor in enabling the rapid and sensitive detection of influenza H7 and other subtypes.

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Functional cure for chronic hepatitis B (CHB) remains challenging due to the lack of direct intervention methods for hepatic inflammation. Multi-omics research offers a promising approach to understand hepatic inflammation mechanisms in CHB. A Bayesian linear model linked gene expression with clinical parameters, and population-specific expression analysis (PSEA) refined bulk gene expression into specific cell types across different clinical phases.

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Dual-mode readout platforms with colorimetric and electrochemiluminescence (ECL) signal enhancement are proposed for the ultrasensitive and flexible detection of the monkeypox virus (MPXV) in different scenes. A new nanotag, Ru@U6-Ru/Pt NPs is constructed for dual-mode platforms by integrating double-layered ECL luminophores and the nanozyme using Zr-MOF (UiO-66-NH) as the carrier, which not only generates enhanced ECL and colorimetric signals but also provide greater stability than that of commonly used nanotags. Dual-mode platforms are used within 15 min from the "sample in" to the "result out" steps, without nucleic acid amplification.

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Drug-induced liver injury in children: A nationwide cohort study from China.

JHEP Rep

August 2024

Division of Gastroenterology and Hepatology, Shanghai Institute of Digestive Disease, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200001, China. Clinical Research Centre, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background & Aims: Currently, there is limited knowledge on the clinical profile of drug-induced liver injury (DILI) in Chinese children. We aimed to assess the clinical characteristics, suspected drugs, and outcomes associated with pediatric DILI in China.

Methods: This nationwide, multicenter, retrospective study, conducted between 2012 and 2014, analyzed 25,927 cases of suspected DILI at 308 medical centers using the inpatient medical register system.

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Hepatitis B Virus (HBV) is a stealthy and insidious pathogen capable of inducing chronic necro-inflammatory liver disease and hepatocellular carcinoma (HCC), resulting in over one million deaths worldwide per year. The traditional understanding of Chronic Hepatitis B (CHB) progression has focused on the complex interplay among ongoing virus replication, aberrant immune responses, and liver pathogenesis. However, the dynamic progression and crucial factors involved in the transition from HBV infection to immune activation and intrahepatic inflammation remain elusive.

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Background: Currently, there are few studies on immune-related prognostic analysis of hepatocellular carcinoma (HCC). Our aim was to establish an immune-correlated prognostic model for HCC.

Methods: Immune-associated cells were obtained from the scRNA-seq dataset (GSE149614) of HCC.

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Article Synopsis
  • Diagnosing tuberculosis (TB) in preterm infants is difficult due to vague symptoms, as illustrated in two recent cases where infants displayed fever and respiratory issues, leading to the eventual diagnosis of TB through PCR testing.
  • In the first case, a 27-week preterm infant developed severe respiratory distress and was confirmed to have TB after treatment for other infections, while his mother was diagnosed with severe TB 92 days postpartum.
  • The second case involved a 36-week preterm infant who, despite vaccination, showed signs of TB after her mother was found to have pulmonary and genital TB, highlighting the need for TB screening in mothers seeking fertility treatments.*
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An inhibitory mechanism of AasS, an exogenous fatty acid scavenger: Implications for re-sensitization of FAS II antimicrobials.

PLoS Pathog

July 2024

Key Laboratory of Multiple Organ Failure, Ministry of Education; Departments of Microbiology and General Intensive Care Unit of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Antimicrobial resistance is an ongoing "one health" challenge of global concern. The acyl-ACP synthetase (termed AasS) of the zoonotic pathogen Vibrio harveyi recycles exogenous fatty acid (eFA), bypassing the requirement of type II fatty acid synthesis (FAS II), a druggable pathway. A growing body of bacterial AasS-type isoenzymes compromises the clinical efficacy of FAS II-directed antimicrobials, like cerulenin.

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