Cloning and Purification of Per a 4, a Gene Encoding a Allergen, and Preparation of Its Monoclonal Antibodies.

Objective: To clone, express, purify the Per a 4 gene encoding an allergen of and prepare monoclonal antibodies against the recombinant allergen.

Methods: The total RNA was extracted from , and the target gene was amplified by RT-PCR and cloned into pMD18-T vector. After being confirmed by nucleotide sequencing, the gene was then inserted into pGEX-3X to construct the express vector pGEX-3X-Per a 4.

Tashinone II A-sulfoacid-natrum elevates the pain threshold through inhibiting nuclear factor kappa B pathway in neuropathic cancer pain.

Objective: The purpose of this study was to evaluate the effects of Tashinone II A-sulfoacid-natrum on the pain threshold and potential molecular mechanism for neuropathic cancer pain.

Methods: Forty-five male Balb/c mice were divided into control group model group and experiment group with each 15. The sciatic nerve muscle plexus of experiment and model group were given injection containing S180 sarcoma cell 2 × 10 6 mL for each mouse.

Prostate cancer antigen 3 as a biomarker in the urine for prostate cancer diagnosis: a meta-analysis.

Objective: The aim of this study was to investigate the clinical value of urine Prostate cancer antigen 3 (PCA3) test in the diagnosis of prostate cancer by pooling the published data.

Methods: The clinical trials about urine PCA3 test in the diagnosis of prostate cancer were searched in the PubMed (January, 1966-July, 2014). Cochrane library (Section 3, 2013), CNKI (March, 1994-July, 2014).

Effects of microRNA-21 on the interleukin 12/signal transducer and activator of transcription 4 signaling pathway in asthmatic mice.

Objective: To study the effect of microRNA-21 (miRNA-21) on the regulation of the interleukin 12 (IL-12)/signal transducer and activator of transcription 4 (STAT4) pathway in the lung tissue of asthmatic mice.

Material And Methods: Forty five male C57BL/6 mice were randomly divided into three groups of 15 mice each: normal control, asthmatic model, and dexamethasone. Our mouse model of allergic asathma was established using OVA sensitization and challenge.