A Quantitative PCR Method to Detect the Tomato Corky Root Rot Pathogens, and .

Corky root rot is an important disease in tomato production systems and is caused by and (formerly Types 1 and 2, respectively). The corky root rot pathogens are slow growing and difficult to isolate and quantify in soil and plant tissue. A multiplex hydrolysis probe-based qPCR assay was designed to allow for simultaneous detection and quantification of and with a competitive internal control to indicate if qPCR inhibitors are present.