Urinary gonadotrophins but not recombinant gonadotrophins reduce expression of VEGF120 and its receptors flt-1 and flk-1 in the mouse uterus during the peri-implantation period.

Background: Ovarian stimulation in humans might affect the perinatal outcome and be considered as a stress factor in the implantation process. In this study we compared the effects of recombinant and urinary gonadotrophins during the mouse peri-implantation period.

Methods: Adult female CD1 mice were treated as follows (a) urinary hFSH and urinary hCG, (b) recombinant hFSH and recombinant hLH and (c) saline.

Estrogen reduces vascular endothelial growth factor(164) expression in the mouse nucleus paraventricularis of the hypothalamus.

The aim of the present study was to establish whether estrogen and corticosteroids exert effects on vascular endothelial growth factor (VEGF)(164) expression in the hippocampus and nucleus paraventricularis of the hypothalamus by in situ hybridization. Female mice were ovariectomized and treated either with estradiol benzoate or vehicle and male mice were either adrenalectomized or sham-operated. Ovariectomy plus estrogen reduced VEGF(164) expression in the nucleus paraventricularis but not in the hippocampus.

Gonadotrophin stimulation reduces VEGF(120) expression in the mouse uterus during the peri-implantation period.

Background: Ovarian stimulation by gonadotrophin treatment exerts negative effects on implantation and embryonic development. We investigated whether gonadotrophin treatment affects VEGF(120) mRNA expression during the peri-implantation period.

Methods: Two groups of adult female CD1 mice were used: the hormone-treated group was injected i.

Maternal deprivation increases 5-HT(1A) receptor expression in the CA1 and CA3 areas of senescent Brown Norway rats.

Maternally-deprived male Brown Norway rats were classified as non-impaired or impaired according to their performance in the water maze when 3 and 30-32 months old. Age and spatial learning ability did not affect the pattern and density of hippocampal 5-HT(1A)-receptor mRNA in mother-reared control rats. However, senescent maternally-deprived rats with impaired spatial learning ability showed increased expression of 5-HT(1A)-receptor mRNA in the hippocampal CA1 (14%) and CA3 (13%) areas but not in the dentate gyrus.