Sample Preparation of Caenorhabditis elegans for GC-MS-Based Metabolomics in Toxicity Assessment.

The nematode Caenorhabditis elegans, widely recognized as a model organism due to its ease of breeding and well-characterized genomes, boasts complete digestive, reproductive, and endocrine systems, as well as conserved signaling pathways shared with mammals. It has become an invaluable resource for metabolomics research, particularly in examining responses to chemical or environmental factors and toxicity assessments. In this article, we provide detailed, step-by-step protocols for cultivating C.

Identification of Primary Metabolites in H NMR-Based Metabolomics of Plants.

Identifying metabolites in NMR-based plant metabolomics is challenging due to the complexity of plant metabolites. This complexity stems from the abundance and diverse chemical properties of compounds, which vary in concentration across plant specimens. Additionally, the lack of automated identification software complicates the analysis process.