2 results match your criteria: "The Netherlands. Electronic address: Jeuken@lic.leidenuniv.nl.[Affiliation]"
Menaquinone-specific turnover by M. tuberculosis cytochrome bd is redox regulated by the Q-loop disulfide bond.
J Biol Chem
December 2024
Leiden Institute of Chemistry, Leiden University, PO Box 9502, 2300 RA, Leiden, The Netherlands. Electronic address:
Cytochrome bd from Mycobacterium tuberculosis (Mtbd) is a menaquinol oxidase that has gained interest as an antibiotic target due to its importance in survival under infectious conditions. Mtbd contains a characteristic disulfide bond that has been hypothesized to allow for Mtbd activity regulation at the enzymatic level, possibly helping M. tuberculosis to rapidly adapt to the hostile environment of the phagosome.
View Article and Find Full Text PDFEnzyme - Switch sensors for therapeutic drug monitoring of immunotherapies.
Biosens Bioelectron
October 2023
School of Biomedical Science, University of Leeds, Leeds, LS2 9JT, United Kingdom; Astbury Centre for Structural Molecular Biology, University of Leeds, LS2 9JT, United Kingdom; Leiden Institute of Chemistry, Leiden University, PO Box 9502, 2300 RA, Leiden, the Netherlands. Electronic address:
Article Synopsis
- Therapeutic monoclonal antibodies (TmAb) are effective treatments for cancers and autoimmune diseases, but their effectiveness can vary greatly between patients, making therapeutic drug monitoring (TDM) important for dosage optimization.
- This study presents a novel enzyme switch sensor platform, combining β-lactamase with custom binding proteins, to rapidly and sensitively measure the levels of two specific TmAbs, trastuzumab and ipilimumab, in blood serum.
- While the sensor successfully detected these two antibodies at low concentrations suitable for therapy, it struggled with other antibodies, highlighting its potential for bedside monitoring and personalized treatment adjustments.