209 results match your criteria: "The Institute of Cytology[Affiliation]"

Article Synopsis
  • The study explored how the timing of starting an obesogenic diet (Cafeteria Diet) affects obesity traits in male and female mice, focusing on pre-puberty and post-puberty initiation.
  • Male mice that started the diet before puberty displayed better metabolic health with lower body weight and improved fat metabolism compared to those starting after puberty.
  • Conversely, female mice starting the diet before puberty showed poorer metabolic profiles, including higher triglycerides and lower insulin sensitivity, showcasing gender differences in diet impact on obesity development.
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Background: In this study, we examined the effectiveness of transfer learning in improving automatic segmentation of brain metastases on magnetic resonance imaging scans, with potential applications in preventive exams and remote diagnostics.

Methods: We trained three deep learning models on a public dataset from the ASNR-MICCAI Brain Metastasis Challenge 2024, fine-tuned them on a small private dataset, and compared their performance to models trained from scratch.

Results: Results showed that models using transfer learning performed better than scratch-trained models, though the improvement was not statistically substantial.

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Machine Learning Algorithms Based on Time Series Pre-Clustering for Nocturnal Glucose Prediction in People with Type 1 Diabetes.

Diagnostics (Basel)

October 2024

Laboratory of Endocrinology, Research Institute of Clinical and Experimental Lymphology, Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (RICEL-Branch of IC&G SB RAS), 630060 Novosibirsk, Russia.

Article Synopsis
  • Machine learning is being used to improve glucose prediction and management for people with type 1 diabetes by analyzing glucose patterns.
  • A study examined continuous glucose monitoring data from 570 adult patients, using hierarchical clustering to identify different glucose dynamics.
  • The results showed that pre-clustering data improved the accuracy of machine learning models, leading to better predictions for both normal glucose levels and low-glucose episodes.
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Implants made of biodegradable polymers are replaced by regenerating tissues through inflammation. The changes occurring in tissues and the organism are of practical interest for studying the biocompatibility of the material and searching for systemic markers in the blood that reflect inflammation in peri-implantation tissues. The highly sensitive C-reactive protein (hs-CRP) measurements in blood and morphometric studies of tissue surrounding the implant were carried out in the experiment within three months of implantation of a biopolymer consisting of polycaprolactone (PCL) and polytrimethylene carbonate (PTMC).

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Article Synopsis
  • - The study focuses on correcting mutations in the CASR gene, which is crucial for regulating calcium levels in the body, specifically targeting mutations linked to a severe condition called neonatal primary hyperparathyroidism.
  • - Researchers generated a CRISPR/Cas9 edited human induced pluripotent stem cell (hiPSC) line that successfully restored one of the mutations while preserving another, showing normal characteristics such as cell structure and growth potential.
  • - The newly created hiPSC line will be used as a valuable resource for understanding calcium regulation disorders and exploring personalized medicine approaches to treat these conditions in the future.
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The expression of markers associated with epithelial-mesenchymal transition (EMT) and extracellular matrix degradation in human uveal melanoma tissue samples and postequatorial zone of the choroid was assessed by immunohistochemical staining. Increased expression of EMT markers E-cadherin and vimentin was observed in the tumor. The ratio of MMP-9 to TIMP-1 proteins related to the extracellular matrix degradation was higher in the tumor.

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We studied cytotoxicity and accumulation of boron and lithium by cultured human fibroblasts and human and mouse skin melanoma cell cultures. The cytotoxicity of boron and lithium drugs was assessed by MTT tests in the boron and lithium concentration range of 10-640 μg/ml. Cell viability was significantly reduced after incubation with boron and lithium at concentrations >160 μg/ml.

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Unlabelled: A biosensor based on field-effect transistors on silicon-on-insulator structures (SOI-biosensor) is a high-potential device for detection of biological molecules, for instance, such as troponin I; the biosensor allows conducting label-free real-time analysis. is the development of SOI-biosensor design for detection of acute myocardial infarction marker - troponin I. A notable feature of this design was the integration of two grounding electrodes directly onto the biosensor surface, which effectively nullified the static potential of the liquid sample and minimized physical breakdowns of biosensor elements.

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Article Synopsis
  • - Osteopoikilosis (OPK) is a rare, benign genetic bone condition characterized by the presence of osteosclerosis foci, and while most cases are asymptomatic, some patients experience joint pain and inflammation.
  • - The case study focuses on a 33-year-old patient with concurrent ankylosing spondylitis (AS) and OPK, highlighting the complications such as severe inflammation and poor response to standard treatments, but a positive reaction to a specific TNF inhibitor.
  • - The report emphasizes the importance of distinguishing OPK from serious conditions like neoplastic lesions, urging doctors to be aware of these rare cases to avoid unnecessary testing.
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The prevalence of overweight and obesity increases in people with type 1 diabetes (T1D). However, the impact of fat accumulation on glucose dynamics in T1D is poorly understood. We assessed continuous glucose monitoring (CGM) parameters in patients with T1D depending on their body weight, body composition, and insulin sensitivity.

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Low intake of micro- and macroelements and vitamins in food negatively affects the health of more than two billion people around the world provoking chronic diseases. For the majority of the world's population, these are soft and durum wheats that provide beneficial nutrients, however their modern high-yielding varieties have a significantly depleted grain mineral composition that have reduced mineral intake through food. Biofortification is a new research trend, whose main goal is to improve the nutritional qualities of agricultural crops using a set of classical (hybridization and selection) methods as well and the modern ones employing gene/QTL mapping, bioinformatic analysis, transgenesis, mutagenesis and genome editing.

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Background: Noninvasive assessment of elevated filling pressure in the left ventricle (LV) remains an unresolved problem. Of the many echocardiographic parameters used to evaluate diastolic pressure, the left atrial strain and strain rate (LA S/SR) have shown promise in clinical settings. However, only a few previous studies have evaluated LA S/SR in larger populations.

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Article Synopsis
  • * Using PCR and pyrosequencing, researchers identified various mtDNA mutations, noting four proatherogenic (harmful) and three antiatherogenic (protective) mutations.
  • * The study also revealed a geographic pattern in the distribution of certain mutations, highlighting a west-east gradient in specific mtDNA mutations associated with atherosclerosis.
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Cells of various organs and systems perform their functions and intercellular interactions not in an inert environment, but in the microenvironment of tissue fluids. Violations of the normal drainage of tissue fluids accompany lymphedema. An important mechanism of angiogenesis and vasculogenesis regulation in tissue fluids is the production and reception of vascular endothelial growth factors in combination with the regulation of matrix metalloproteinases.

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The escalating threat of multidrug-resistant pathogens necessitates innovative approaches to combat infectious diseases. In this study, we examined peptides R23F*, V31K*, and R44K*, which were engineered to include an amyloidogenic fragment sourced from the S1 protein of , along with one or two cell-penetrating peptide (CPP) components. We assessed the antimicrobial efficacy of these peptides in a liquid medium against various strains of both Gram-positive bacteria, including (209P and 129B strains), MRSA (SA 180 and ATCC 43300 strains), and (strain IP 5832), and Gram-negative bacteria such as (ATCC 28753 and 2943 strains) and (MG1655 and K12 strains).

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URINARY EXCRETION OF ALPHA-ACTININ-4 AND TIGHT JUNCTION PROTEIN 1 IN PATIENTS WITH TYPE 2 DIABETES AND DIFFERENT PATTERNS OF CHRONIC KIDNEY DISEASE.

Georgian Med News

March 2024

Research Institute of Clinical and Experimental Lymphology, Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, (RICEL-Branch of IC&G SB RAS), Novosibirsk, Russia.

Abnormalities of the cytoskeleton and the slit diaphragm of podocytes have been attributed to diabetic nephropathy. In this study, we assessed urinary excretion of alpha-actinin-4 (ACTN-4), a cytoskeleton protein and a component of the slit diaphragm, and tight junction protein 1 (TJP-1, or ZO-1), a peripheral membrane protein that forms molecular complexes with actin filaments, in patients with type 2 diabetes (T2D) and albuminuric or non-albuminuric chronic kidney disease (CKD). The study included 140 patients with long-term T2D (≥10 years) and 20 healthy subjects as control.

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Lymphedema is a disorder that leads to excessive swelling due to lymphatic insufficiency, resulting in the accumulation of protein-rich interstitial fluid. Primary lymphedema predominantly impacts the lower extremities and is frequently linked to hereditary factors. This condition is known to be associated with variants in several genes, such as , , and .

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Acute myeloid leukemia (AML) is a malignant neoplasm characterized by extremely low curability and survival. The inflammatory microenvironment and maturation (differentiation) of AML cells induced by it contribute to the evasion of these cells from effectors of antitumor immunity. One of the key molecular effectors of immune surveillance, the cytokine TRAIL, is considered a promising platform for developing selective anticancer drugs.

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β-tricalcium phosphate (β-TCP) is a promising material in regenerative traumatology for the creation of bone implants. Previously, it was established that doping the structure with certain cations can reduce the growth of bacterial activity. Recently, much attention has been paid to co-doped β-TCP, that is explained by their ability, on the one hand, to reduce cytotoxicity for cells of the human organism, on the other hand, to achieve a successful antibacterial effect.

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Glucose management at night is a major challenge for people with type 1 diabetes (T1D), especially for those managed with multiple daily injections (MDIs). In this study, we developed machine learning (ML) and deep learning (DL) models to predict nocturnal glucose within the target range (3.9-10 mmol/L), above the target range, and below the target range in subjects with T1D managed with MDIs.

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Aim: To study ischemic and/or nonspecific ECG signs of metabolic changes in the myocardium and to determine their relationship with unfavorable cardiovascular prognosis in a 7-year prospective observation of young people under 45 years of age.

Material And Methods: A cross-sectional population survey of a random sample aged 25-44 years (n=1363) was conducted in Novosibirsk. The survey program used the standardized epidemiological Rose questionnaire.

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Dysregulation of cell cycle, proliferation, and autophagy plays a pivotal role in diabetic kidney disease. In this study, we assessed urinary excretion of molecular regulators of these processes that mediate their effects via the PI3K/AKT/mTOR pathway in subjects with long-term type 2 diabetes (T2D) and different patterns of chronic kidney disease (CKD). We included 140 patients with T2D and 20 non-diabetic individuals in a cross-sectional study.

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Objective: To study the relationship of polymorphic variants of the SLC6A4 gene with depression among people aged 25-44 years in Novosibirsk.

Material And Methods: Under the WHO program «MONICA-psychosocial (MOPSY)», a random representative sample of people aged 25-44 years from the population of the Oktyabrsky district of Novosibirsk (men =725, mean age 43.4±0.

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This study examined the effectiveness of coating demineralized bone matrix (DBM) with amorphous calcium phosphate (DBM + CaP), as well as a composite of DBM, calcium phosphate, and serum albumin (DBM + CaP + BSA). The intact structure of DBM promotes the transformation of amorphous calcium phosphate (CaP) into dicalcium phosphate dihydrate (DCPD) with a characteristic plate shape and particle size of 5-35 µm. The inclusion of BSA in the coating resulted in a better and more uniform distribution of CaP on the surface of DBM trabeculae.

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Article Synopsis
  • - Doubled haploid technology aids plant breeding by quickly producing homozygous lines through anther culture, allowing for efficient variety development.
  • - The study evaluated the androgenesis response of various wheat cultivars and F1/F2 hybrids from the Siberian Research Institute, focusing on factors such as new plant formations and regeneration success.
  • - Results indicated significant variability among genotypes, with Novosibirskaya 15 showing high responsiveness to anther culture, while Novosibirskaya 31 and 16 exhibited lower regeneration capabilities, highlighting the potential of Novosibirskaya 15 in future breeding programs.
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