Bioinformatics- and quantitative proteomics-based identification of gastric adenocarcinoma-related proteins and analysis.

Background: The emergence of immune resistance and a lack of effective therapeutic targets have become significant challenges in immunotherapy, highlighting the urgent need for new molecular markers and treatment targets. Moreover, the significance and mechanisms of PGRN (Progranulin) in gastric cancer remain ambiguous.

Objective: To identify differentially expressed proteins in gastric cancer and elucidate the function and mechanism of PGRN.

Establishment and verification of a predictive model for bone metastasis in patients with non-small cell lung cancer based on peripheral blood CX3CL and CCL28.

To establish a predictive model of bone metastasis in patients with non-small cell lung cancer (NSCLC) using peripheral blood CX3CL and CCL28, and to verify its application value. We retrospectively gathered clinical data from 210 patients with NSCLC treated at our institution between April 2021 and December 2023. These patients were stratified into two groups based on the presence of bone metastases: a bone metastasis group (n = 49) and a non-bone metastasis group (n = 161).

Recurrent metastatic patterns and prognosis after radical surgery in patients with alpha-fetoprotein-producing gastric cancer: a retrospective cohort study.

Alpha-fetoprotein-producing gastric cancer (AFPGC) is a rare and aggressive subtype of gastric cancer associated with poor prognosis. This study aimed to investigate the recurrent metastatic patterns and prognostic factors in AFPGC patients undergoing radical surgical resection. Data from 241 AFPGC patients diagnosed between January 2017 and January 2020 who underwent surgical resection were analyzed across multiple centers.

Safety, tolerability, and pharmacokinetics of mitoxantrone hydrochloride injection for tracing in patients with gastric cancer: a single-blind, single-center, phase I clinical trial.

Mitoxantrone Hydrochloride Injection for Tracing (MHI), a modified new drug marketed in China, has been approved by the National Medical Products Administration for lymph node tracing in thyroid cancer and sentinel lymph node biopsy in breast cancer. This single-center, single-blind, dose-escalation phase I clinical trial aimed to investigate the safety of MHI on lymph node tracing in gastric cancer. In this study, four dose groups (1.

Compliance of postoperative gastric cancer patients with oral nutritional supplementation and its influencing factors.

Objectives: To evaluate the compliance of postoperative gastric cancer patients with oral nutritional calcium supplementation and explore its influencing factors, in order to provide a reference for formulating relevant nursing interventions.

Methods: A total of 269 postoperative patients with gastric cancer admitted to the third department of surgery of the Fourth Hospital of Hebei Medical University from February to July 2020 were selected retrospectively through convenient sampling. A general information questionnaire and the Chinese version of the modified medication adherence eight-item scale were used to conduct a cross-sectional survey, in order to evaluate the compliance of postoperative gastric cancer patients with oral nutritional supplementation.

Comprehensive imaging and pathology of solitary patellar metastasis from breast cancer with literature review on its diagnosis and prognosis.

Background: Breast cancer is the most frequent non-skin cancer in women and bone is its most common site of metastasis. The patella, as the largest sesamoid bone in the human body, is a rare site for cancer metastasis to occur. We reported the comprehensive auxiliary examination data and complete process of diagnosis, management and follow-up for a case of solitary patellar metastasis from breast cancer.

A predictive model for postoperative cognitive dysfunction in elderly patients with gastric cancer: a retrospective study.

Objective: To explore the risk factors of postoperative cognitive dysfunction (POCD) in elderly patients with gastric cancer after radical resection and to establish a risk prediction model.

Methods: A retrospective analysis of the clinicopathological data of 687 elderly patients who underwent radical gastric cancer surgery from January 2014 to January 2020 in the Third Department of Surgery, Fourth Hospital of Hebei Medical University was conducted. The degree of cognitive impairment was divided into POCD positive group (n=141, 20.

Prognostic nomogram for Siewert type II adenocarcinoma of the esophagogastric junction patients with and without neoadjuvant radiotherapy: a retrospective cohort study.

Objective: To compare the prognostic factors of Siewert type II AEG patients who had received neoadjuvant radiotherapy (nRT) versus those who did not receive nRT. Nomograms for outcome prediction were constructed for the two treatment modalities.

Materials And Methods: Data for 1,745 Siewert II type AEG patients who underwent radical surgery between 2010 and 2015 were retrieved from SEER (Surveillance, Epidemiology, and End Results) database.

Breast cancer with an initial gastrointestinal presentation: a case report and literature review.

The most common sites of breast cancer metastasis are the lymph nodes, lungs, bones, and liver. Gastrointestinal (GI) metastasis is relatively rare and often occurs within several years after a breast cancer diagnosis. Most patients experience abdominal pain, anorexia, bleeding, vomiting, and other digestive system symptoms, symptoms which are difficult to distinguish from primary gastric cancer.

[Effect of EDTA and hyaluronic acid on the activity of periodontal ligament fibroblasts and the expression of cytokines].

Purpose: To investigate the effect of ethylenediaminetetraacetic acid (EDTA) and hyaluronic acid (HA) on the activity of periodontal ligament fibroblasts (PDLFs) and the expression of cytokines.

Methods: Twelve wisdom teeth extracted due to orthodontics treatment were selected and prepared into 5 mm×4 mm root pieces, then divided into EDTA group, HA group, EDTA+HA group and untreated group according to different treatment methods. They were placed in the well plate and PDLFs inoculated on the root piece.

[Expression and clinical significance of Spindly and Bub3 in oral squamous cell carcinoma].

Purpose: To investigate the expression and significance of Spindly and Bub3 in oral squamous cell carcinoma(OSCC).

Methods: Sixty-five patients with OSCC admitted to the Fourth Hospital of Hebei Medical University from March 2017 to March 2019 were enrolled. RT-PCR was used to detect the expression of Spindly and Bub3 mRNA in oral squamous cell carcinoma and adjacent normal tissues from the patients.

Assessment of a developed HPLC-MS/MS approach for determining plasma eupatorin in rats and its application in pharmacokinetics analysis.

Eupatorin, a bioactive compound extracted from Java tea (), possesses potent anti-cancer, anti-inflammatory and vasodilation activities. To date, no pharmacokinetics studies on eupatorin have yet been performed. Here, we established and validated a sensitive and selective LC-MS/MS (liquid chromatography-tandem mass spectrometry) approach for determining plasma eupatorin in rats.

Inhibitory effect of siRNA-Annexin A7 on growth, migration, and invasion in BGC823 cells and gastric cancer xenograftsin nude mice.

Objective: To explore the inhibitory effect of siRNA-Annexin A7 on growth, migration, and invasion of transplanted gastric cancer in nude mice.

Methods: The siRNA sequence targeting to human Annexin A7 gene was designed, and based on that a pair of complementary oligonucleotides were synthesized, annealed, and cloned into plasmid pGenesil-1.1 to construct recombinant plasmid siRNA-Annexin A7.

The development and validation of an HPLC-MS/MS method for the determination of eriocitrin in rat plasma and its application to a pharmacokinetic study.

Eriocitrin is one of the major active constituents of lemon fruit, and it possesses strong antioxidant, lipid-lowering, anticancer and anti-inflammatory activities and has long been used in food, beverages and wine. In this study, for the first time, a rapid, selective, and sensitive liquid chromatography-tandem mass spectrometry method (LC/MS/MS) with protein precipitation was developed and validated for the analysis of eriocitrin in rat plasma. Chromatographic separation was achieved using a mobile phase, comprising 0.

Downregulation of annexin A7 decreases proliferation, migration, and invasion of gastric cancer cells by reducing matrix metalloproteinase 1 and 9 expression.

High annexin A7 expression is a potential indicator of lymphatic metastasis and poor prognosis in patients with gastric cancer (GC). The mechanism underlying the effects of annexin A7 on GC cells remains unclear. In patients with GC, primary adenocarcinoma tissues had higher annexin A7 expression than adjacent non-cancerous tissues ( < 0.

Isolation and characterization of CD105+/CD90+ subpopulation in breast cancer MDA-MB-231 cell line.

Background: The epithelial-mesenchymal transition (EMT) generates cells with properties of stem cells, if that happened, the stem cell should be with mesenchymal property. This study aimed to identify a group of cells with mesenchymal stem cell (MSC)-like characteristics in breast cancer bone metastatic cell line MDA-MB-231, moreover, the relevance between breast cancer stem cells and the EMT was observed. CD105 and CD90, identified as the standards of MSCs, were used for the identification.

MicroRNA-148b is down-regulated in non-small cell lung cancer and associated with poor survival.

Background: The aim of this study was to clarify the clinicopathological significance of miRNA-148b (miR-148b) expression in NSCLC, and to explore the correlation between miR-148b level and the prognosis of patients with NSCLC.

Methods: 151 patients diagnosed with NSCLC between May 2007 and April 2012 were included in the present study. Real-time RT-PCR method was used to assess the expression levels of miR-148b.

Expression of annexin A7 and its clinical significance in differentiation and metastasis of gastric carcinoma.

Objective: To investigate the expression and clinical significance of annexin A7 in the differentiation and lymphatic metastasis of gastric cancer (GC).

Methods: The clinical and pathological data were recorded for analysis. Immunohistochemical staining and Western blot were performed to analyze the expression of ANXA 7 in primary GC tissues.