Fully automated synthesis module for preparation of S-(2-[(18)F]fluoroethyl)-L-methionine by direct nucleophilic exchange on a quaternary 4-aminopyridinium resin.

A fully automated preparation of S-(2-[(18)F]fluoroethyl)-L-methionine (FEMET), an amino acid tracer for tumor imaging with positron emission tomography, is described. [(18)F]F(-) was produced via nuclear reaction (18)O(p,n) [(18)F] at PETtrace Cyclotron. Direction nucleophilic fluorination reaction of [(18)F]fluoride with 1,2-di(4-methylphenylsulfonyloxy)ethane on a quaternary 4-(4-methylpiperidinyl)pyridinium functionalized polystyrene anion exchange resin gave 2-[(18)F]-1-(4-methylphenyl-sulfonyloxy)ethane, and then [(18)F]fluoroalkylation of L-homocysteine thiolactone with 2-[(18)F]-1-(4-methylphenylsulfonyloxy)ethane yielded FEMET.

[A study on the relation between the location of brain damage and agraphia of Chinese].

Objective: To investigate the relation between the location of brain damage and Chinese agraphia.

Methods: Aphasia Battery of Chinese (ABC) and Chinese Agraphia Battery (CAB) were used to examine the ability of oral language and writing. Different types of aphasia and agraphia were detected.

[One-stage surgical management for spinal tuberculosis].

Objective: To evaluate the clinical efficacy of one-stage surgical management for spinal tuberculosis.

Methods: Fifty-seven patients with thoracic, thoracolumbar and lumbar spinal tuberculosis were treated surgically by a single stage procedure from Jan 1996 to Jan 2001, including anterior resection, interbody outografting and anterior instrumentation in 35 cases, posterior debridement and posterior instrumentation in 16 cases, and posterior instrumentation and anterior resection plus interbody autografting in 6 cases. Titanium-alloy spinal implants were used in 38 cases and stainless steel implants in other 19 cases.

[PKC-dependent activation of P44/42 MAPKs and HSP70 in signal transduction pathways during hepatocyte ischemic preconditioning].

Objective: To investigate the significance of PKC, P44/42 MAPKs and HSP70 signal transduction in IP.

Methods: Through an in vivo rat liver IP model, PKC inhibitor, activator and MEK inhibitor were utilized to analyze the phosphorylation of PKC and P44/42 MAPKs. HSP expression, AST/ALT concentration, cellular structure and ultrastructure were also observed.

Effects of KAI1/CD82 on biological behavior of human colorectal carcinoma cell line.

Aim: To investigate the effects of KAI1/CD82 on biological behavior of colorectal carcinoma cells.

Methods: KAI1 cDNA was transfected into highly malignant colorectal carcinoma cell line, LoVo, which had low level of endogenous KAI1 expression, and established stable transfectant clones with high KAI1/CD82 expression. The cell-cell adhesion, cell aggregation, cell-matrix adhesion and cell invasion assay were performed to determine whether KAI1 transfectant could have an effect on proliferation, adhesion and tumor metastasis in comparison with the control transfectant cells.

[The TNF-alpha-induced apoptosis of LA795 cells is mediated by MAP kinase kinase 6].

Objective: To study the molecular mechanism of apoptosis of lung cancer cells stimulated by tumor necrosis factor alpha for the discovery of potential methods of gene therapy of lung cancer.

Methods: The apoptosis model of LA795 adenocarcinoma cells was performed. Recombinant adenovirus of MAP kinase kinase 6, and its constitutively active form and dominant negative form were prepared in 293 package cells.

Ribozyme targeted on HPV16E6 mRNA induced apoptosis on human cervical carcinoma CaSKi cells.

Background & Objective: Human papillomavirus is related to cervical cancer. Ribozyme is special kind of RNA that can cleave target RNA. The aim of this study was to investigate the characterization of the cultured cervical cancer cell line transfected with anti-HPV16E6-ribozyme (HRz) and the effect of ribozyme on proliferation and apoptosis of cervical cancer cell.

[Animal model of rat-to-mouse xenogeneic bone marrow transplantation with graft-versus-host disease].

To observe the graft-versus-host disease (GVHD) in rat-to-mouse model of bone marrow transplantation to build a GVHD model, BALB/c mice were conditioned with 8.5 Gy lethal total body irradiation and divided into two groups. One group of mice was infused with 4 x 10(7) bone marrow cells (BMC) from SD rats.

Properties of several glycidyl methacrylate-triallyl isocyanurate based affinity adsorbents for removing circulating immune complexes.

Biocompatible affinity adsorbents prepared from macroporous glycidyl methacrylate-triallyl isocyanurate copolymer (GT) has been used for removing circulating immune complexes (CICs). In this work, adsorption of circulating immune complexes on GT-based affinity adsorbents has been studied by using batch and hemoperfusion studies. In batch mode, the equilibrium adsorption level was determined to be a function of the contact time, temperature, initial CIC concentration and adsorbent dosage.

Recombinant Helicobacter pylori catalase.

Aim: To construct a recombinant strain which highly expresses catalase of Helicobacter pylori (H. pylori) and assay the activity of H. pylori catalase.

[Determination of clonal T cell receptor gene rearrangement in non-Hodgkin's lymphoma patients and its clinical significance].

Background & Objective: The aim of this study was to investigate the clonal T cell receptor (TCR) gene rearrangement in the patients with non-Hodgkin's lymphoma (NHL) and its clinical significance.

Methods: TCRVgammaI-Jgamma gene rearrangement were detected in 43 patients with polymerase chain reaction (PCR) and single-strand conformation polymorphism (SSCP).

Results: Of 43 cases of NHL, 26 (60.

[Influence of irradiation on the dynamic three-dimension distribution of abl and bcr genes in the interphase nuclei of IM-9 cell].

Objective: To investigate the material foundation of the fusion of bcr and abl genes, and to explore the pathogenesis of chronic myeloid leukemia.

Methods: By FISH combined with laser confocal scanning microscopy, the three-dimension (3D) distribution of bcr and abl genes in the interphase nuclei of normal and irradiated IM-9 cells was studied in each cell cycle phases.

Results: abl and bcr genes distributed non-randomly in the interphase nuclei of IM-9 cells.

[Effect of activation of cellular immunity on p58+ cells expressing killer-cell-inhibitory receptor cells].

Unlabelled: The purpose of this study was to evaluate the effects of cellular immunity activation on P58(+) cells expressing killer cell inhibitory receptor (KIR) and their regulatory function on cellular immunity, and provid theoretical data for preventing graft-vers-host disease (GVHD) in stem cell transplantation therapy. The mononuclear cells from human peripheral blood were incubated with IL-2, Con A and Lipostin (LP) for 72 hours. The KIR expressing cells, P58.

[The distributing characteristics and proliferating activity of fibroblasts from the surrounding skin of keloids].

Objective: To investigate whether there are abnormal fibroblasts derived from the surrounding skin of keloids so that a more accurate therapy for keloids could be obtained.

Methods: Samples were taken for cell culture. When primary cells fully covered the culture bottle, the shape and distributing characteristics of fibroblasts were observed under the light microscope.

[The application of dual fusion interphase fluorescence in situ hybridization probe in detecting minimal residual disease in chronic myeloid leukemia].

Objective: To explore the sensitivity, specificity and reproducibility of dual-color and dual-fusion fluorescence in situ hybridization (D-FISH) in detecting the minimal residual disease (MRD) of chronic myeloid leukemia (CML) after treatment.

Methods: MRD were detected in the bone marrow of 8 cases of chronic myeloid leukemia after treatment with interphase fluorescence in situ hybridization. Sensitivity, specificity and reproducibility of D-FISH were compared with those of single-fusion FISH (S-FISH).

[Analysis of gene expression patterns of leukemia K562 cells after cytochalasin B treatment].

Background & Objective: The advanced technique of DNA microarray makes it possible to monitor the expression of thousands of genes simultaneously in one hybridization experiment. This technique accelerates demonstration of anti-tumor drug mechanisms and discovery of new drug targets. This study was designed to investigate the differential gene expression of K562 cells after cytochalasin B treatment using cDNA microarray.

[The applied anatomy of axial subdermal vascular network skin flap and its application in the management of deep burn wound].

Objective: To explore the anatomical basis and clinical application of axial subdermal vascular network skin flap (ASVNF) in the management of deep burn wounds.

Methods: Twelve samples of skin and subcutaneous tissue of lower extremities from adult cadavers were anatomically observed. The course, branching and the architecture of the subdermal vascular network (SVN) in the superficial facia and the ultra-thin area of the anterolateral femoral and saphenous axial subdermal vascular network skin flaps were intensively studied.

Psychological consequences derived during process of human hand allograft.

Objective: To study the psychology and the treatment during the process of hand allograft.

Methods: The patients were interviewed to evaluate their states of mind and their abilities to manage stress during the selection of patients. The psychology of the two patients were trained before the operation and managed accordingly afterwards.

[Applied anatomy of abut structures of internal auditory meatus (IAM) through middle cranial fossa approach].

Objective: To provide anatomical basis for operation of internal auditory meatus(IAM). Correlation structures with IAM were measured according to sign of hiatus of facial nerve.

Method: These structures include spinous foramen, superficial geater petrosal nerve, superficial lesser petrosal nerve, hiatus of facial nerve were measured on 24 wet cranial bone (48 sides).

[Immunohistochemical detection of micrometastases in cervical lymph nodes from squamous cell carcinoma of tongue in neck dissection specimens].

Objective: The purpose of this study was to explore a feasible method to detect the micrometastases.

Methods: Totally 152 cases of negative cervical lymph nodes (CLNs) from 30 patients with squamous cell carcinoma in tongue were included in this study. The HE-stained slices of the CLN were reexamined by two experienced pathologists and, conformed that no carcinoma cells were found.

New component of the limbic system: Marginal division of the neostriatum that links the limbic system to the basal nucleus of Meynert.

The limbic system refers to a group of connected neural regions that are associated with motivation, learning, and memory. The marginal division (MrD) is a zone located at the caudal border of the neostriatum in mammalian brains that has been shown to be involved in learning and memory. In a previous study, c-fos expression showed functional connections between the MrD, basal nucleus of Meynert (NBM) and limbic system (Shu et al.

[Study on Methylation of p15(INK4B) Gene in Acute Myeloid Leukemia and Chronic Myeloid Leukemia].

In order to explore the role of p15(INK4B) gene with highly methylated CpG island in the pathogenesis of leukemia, the expression levels of p15(INK4B) gene was detected in patients with AML and CML. Methylation-specific PCR (MSP) assay was employed in the experiments. The methylation incidence was 83.

[Research of Typing for HLA-A, -B on Cord Blood Lymphocytes].

Serological typing for HLA-A, -B has been used for a long time. Recently with the developing of molecular biology technologies, HLA-A, -B typing is now turning to genotyping methods. In our study, the capacity of PCR-SSP in solving problems in HLA-A, -B typing with serological methes was evaluated.