[Study on serum p53 protein in cops in Guangzhou city].

Objective: Serum p53 protein overexpression was detected in population exposed to traffic exhaust gas to study the relation between traffic exhaust gas and the increased risk in p53 gene mutation.

Methods: Serum p53 protein expression was measured by enzyme-linked immunosorbent assay. Relationship between different types of job and serum p53 protein overexpression were studied by pearson Chi-square tests.

[Nitric oxide is involved in the modulation of central respiratory rhythm].

This experiment was expected to test whether nitric oxide (NO) exerted significant effect on the central respiratory rhythm. Experiments were performed on in vitro brainstem slice preparations from neonatal rats. These preparations include the medial region of the nucleus retrofacialis (mNRF); a part of pre-Bötinger complex, ventral respiratory group (VRG) and dorsal respiratory group (DRG).

[Herbalogical study on tianxiong].

According to textual study of herbal drugs and modern scientific research on Tianxiong, the long biennial root tuber losing reproductive capacity of Aconitum carmichaeli Debx. is regarded as the orthodox medicinal herbs through the ages.

A preliminary pathological study on human allotransplantation.

Objective: To observe the survival of hand allograft under the state of immunosuppression and the pathological changes of rejection in the recovery process.

Methods: The biopsies of the skin, nerve, muscle, tendon and bone tissue of hand allografts during different stages from 1 day to 7 months after operation were observed using routine histological technique.

Results: No significant changes due to rejection in skin, nerve, muscle and bone tissue were observed.

Synthesis and evaluation of O-(3-[18F]fluoropropyl)-L-tyrosine as an oncologic PET tracer.

O-(3-[(18)F]fluoropropyl)-L-tyrosine (FPT), an analogue of O-(2-[(18)F]fluoroethyl)-L-tyrosine (FET) as an amino acid tracer for tumor imaging with positron emission tomography (PET), was synthesized and evaluated. FPT was prepared by [(18)F]fluoropropylation of L-tyrosine in a two-step procedure. Biodistribution of FPT was determined in normal mice.

[Suppression subtractive hybridization and its application in study of tumors].

Suppression subtractive hybridization (SSH) has been recently reported as a differential display technique at the basis of suppressive polymerase chain reaction(PCR). This technique has such advantages as easy and feasible, highly specific, capable of separating the low abundant specific fragments. Now it has been successfully applied in the study of tumors, developing biology, immune regulations, etc.

[Application of interphase fluorescence in situ hybridization for determination of the tumor load in chronic myeloid leukemia].

Background & Objective: It is essential to evaluate the tumor burden accurately in assessing the degree of response of chronic myeloid leukemia for design of treatment model. The aim of this study was to explore the sensitivity and specificity of interphase fluorescence in situ hybridization (I-FISH) technique in monitoring the tumor load of chronic myeloid leukemia (CML) during treatment.

Methods: Ten cases of normal bone marrow cells were examined using I-FISH technique to define the normal cut-off value.

Expression of angiotensin II type 1 receptor in rat hepatic stellate cells and its effects on cell growth and collagen production.

Objective: To investigate the contribution of angiotensin II towards the process of hepatic fibrosis that is largely due to hepatic stellate cell growth.

Methods: Adult rat hepatic stellate cells were cultured and checked for the expression of angiotensin II receptor 1a (AT(1a)) mRNA by RT-PCR and sequence analysis. The effects of angiotensin II were observed on stimulation of hepatic stellate cell growth detected by MTT assays, (3)H-thymidine incorporation and cell count, and collagen synthesis by (3)H-proline incorporation.

[Human telomerase reverse transcriptase as a novel target for tumor immunotherapy].

Recently, a new immunotherapy against cancer based on a novel tumor-associated antigen of the human telomerase reverse transcriptase (hTERT) is being investigated. Antigen peptides derived from hTERT are expressed and presented with major histocompatibility complex (MHC) class I molecules in tumor cells, and elicit ex vivo cytotoxic T-lymphocyte (CTL) responses in healthy individuals and cancer patients. Data from both human and murine systems demonstrate that TERT-special CTL kill TERT-positive tumor cells of various histological origins in a MHC-restricted fashion.

[Treatment of refractory leukemia by combined chemotherapy and halpotype lymphocytes infusion].

Background & Objective: The complete remission of refractory leukemia treated with conventional chemotherapy is below 50 percent. The high dose chemotherapy can cause more mortality of patients with refractory leukemia. Cytolysis of leukemia cells induced by halpotype lymphocytes was observed in vitro in our previous experiment.

[Glivec in combination with HA regimen for treatment of 20 patients with Ph chromosome positive acute leukemia].

Background & Objective: Glivec was approved by Food & Drug Administration (FDA) in May 2001 as a gene target drug for treatment of chronic myeloid leukemia (CML) and showed a good curative effect for patients with chronic myeloid leukemia in chronic phase. But its effectiveness was poor in patients with CML blast phase treated with Glivec alone. Glivec was reported having synergetic effect with other chemical agents in vitro, but there is few report in clinical combined application.

[Sodium butyrate-induced expression of lung resistance-related gene in human myeloid leukemia K562 cells].

Background & Objective: Overexpression of lung resistance-related protein(LRP) is involved in multidrug resistance and poor outcome in acute leukemia. This study was designed to investigate the effect of sodium butyrate (NaB) on LRP expression level and the function of LRP in K562 cells.

Methods: Human myeloid leukemia K562 cells, used as an in vitro model, were treated with NaB.

Progress in DNA chip technology.

DNA chip technology employs light-directed in situ oligonucleotide synthesis and/or DNA microarray printing device to produce arrays of large number of probes in the tiny surface of silicon substrates, which makes it possible that the gene detection be conducted efficiently with high speed and sensitivity. The DNA chip may take important part in genome research, gene diagnoses and so on.

Analysis of the gene expression profile of Schistosoma japonicum cercariae by a strategy based on expressed sequence tags.

We present an analysis of the expression profile of Schistosoma japonicum cercariae by a strategy based on expressed sequence tags (ESTs). A cDNA library from S. japoniucm cercariae was constructed and was used to generate ESTs.

[Study on etiology and pathology of severe acute respiratory syndrome].

Objective: To investigate the clinicopathologic characteristics of severe acute respiratory syndrome (SARS).

Methods: Three autopsy cases were studied retrospectively. Routine HE stain was used to study all the cases.

Expression profile of trophoblast invasion-associated genes in the pre-eclamptic placenta.

The primary pathology of pre-eclampsia is thought be a defect in placentation due to failure of trophoblast invasion. Here, we aim to identify the expression profile of invasion-associated genes in the pre-eclamptic placenta. Messenger RNA (mRNA) expression levels of extracellular matrix molecule-related genes in five pre-eclamptic placentas and in five strictly matched normal placentas were assayed using complementary DNA (cDNA) microarrays representing over 220 human cytokine-associated or hormone-associated genes.

[Experimental study of optimized H-2 haploidentical hematopoietic engraftment for the treatment of murine acute leukemia model].

Objective: To explore the critical dose of T lymphocyte for preserving graft versus leukemia (GVL) while preventing GVHD in murine acute leukemia model treated with H-2 haploidentical hematopoietic stem cell transplantation (HSCT).

Methods: (C57BL/6 x 615) F1 (H-2bk) mice which was inoculated with L615 cells to develop leukemic murine model was the recipient. The healthy C57BL/6 (H-2b) mice was the donor.

[Detection of bcr/abl fusion gene in chronic myeloid leukemia by fluorescence in situ hybridization and nested reverse transcription polymerase chain reaction].

Objective: To detect the bcr/abl fusion gene in chronic myeloid leukemia (CML) for assisting in clinical diagnosis.

Methods: Fluorescence in situ hybridization (FISH) and nested reverse transcription polymerase chain reaction (RT-PCR) were used.

Results: Out of 16 CML patients, the results of FISH was not consistent with that of nested RT-PCR in 3 cases.

[Study of murine hematopoietic stem/progenitor cell mobilized by recombinant human interleukin 11 combination with granulocyte-colony stimulating factor].

Objective: To study the recombinant human interleukin 11 (rhIL-11) in combination with granulocyte-colony stimulating factor (rhG-CSF) for mobilizing peripheral blood stem/progenitor cells in C57BL/6 mice.

Methods: rhIL-11,250 micro g x kg(-1) x d(-1) per mouse alone or in combination with rhG-CSF 250 micro g x kg(-1) x d(-1) per mouse was administered to C57BL/6 mice from day 1 to 7. The changes of peripheral white blood cell count (WBC), platelet counts (BPC) and hematopoietic stem/progenitor cells yields were observed.

Neuroprotective effects of Buyang Huanwu Decoction on neuronal injury in hippocampus after transient forebrain ischemia in rats.

Buyang Huanwu Decoction (BYHWD), a traditional Chinese medicine, has been developed as a drug to be used for treatment of stroke for hundreds of years. However, the underlying mechanisms remain unknown. In the present study, the effects of BYHWD on delayed neuronal death of hippocampus after transient forebrain ischemia were examined in rats.

[ABO blood group typing for infants and its application for clinical transfusion].

To study the correct method for determining ABO blood types in infants and its influencing factors, blood types of 33 infants under 6 months old were determined by routine serological method, micro-column gel typing system and PCR-SSP genotyping method. Of the 33 cases with discrepant results of ABO blood type by different methods, the blood types of 32 cases were discrepant between red cell and serological typings in the routine serological method, and a false coincidence in 1 case was caused by bacterial infection resulting in B-like antigen. Correct blood typing was obtained in 27 cases with a correct rate of 84.

[Treatment of malignant hematologic diseases by peripheral blood stem cell transplantation combined with halotype lymphocyte infusion].

In order to observe the curative and side effects in malignant hematologic diseases treated with autologous peripheral blood stem cell transplantation (auto-PBSCT) combined with halotype lymphocyte infusion, auto-PBSCs were mobilized, harvested and stored at -196 degrees C from patients in first CR or PR with intensive chemotherapy (Ara-C 1.0 g/m(2) x 5 days or cyclophosphamide 60 mg/kg x 2 days) and G-CSF. Unpurged auto-PBSCs were infused when patients received the conditioning regimen with busulfan, total irradiation or cyclophosphamide.

[Association of HLA-DPB1 alleles with chronic myelogenous leukemia in southern Chinese Hans].

To clarify the association between HLA-DPB1 alleles and chronic myelogenous leukemia (CML) in South Chinese, the allelic types of HLA-DPB1 were detected by sequence based typing (SBT) in 86 patients with CML and 82 healthy individuals from Southern China. The results showed that the frequencies of HLA-DPB1 * 1301 and DPB1 * 20011 were higher in patients with CML in comparison with those of healthy individuals. It is concluded that positive association may exist between certain HLA-DPB1 alleles and CML.

[In vitro effect of STI571 on expression of c-kit in bone marrow cells from patients with acute non-lymphocytic leukemia].

This study was designed to explore the influence of STI571, a tyrosine kinase inhibitor, on the expression of c-kit in the bone marrow cells from patients with acute non-lymphocytic leukemia (ANLL). The cells were exposed to various concentration of STI571 for 72 hours, the expression of c-kit mRNA and CD117 was assayed by RT-PCR and flow cytometry, respectively. The results showed that STI571 treatment induced concentration-dependent decrease of c-kit and CD117 expression, which was significant lower than that in group before treatment and untreated control groups (P < 0.