GDF15 negatively regulates RGS16 to impair hepatic lipid metabolism in male mice offspring conceived by in vitro fertilization.

Objectives: We generated an in vitro fertilization and embryo transfer (IVF-ET) mouse model to investigate the molecular mechanism underlying the abnormal lipid metabolism found in IVF-ET offspring.

Methods: The glucose metabolism levels of offspring were assessed by glucose tolerance test (GTT), insulin tolerance test (ITT), and pyruvate tolerance test (PTT). The lipid metabolism levels were assessed by triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C).

Brachyury promotes proliferation and migration of hepatocellular carcinoma via facilitating the transcription of NCAPG2.

Hepatocellular carcinoma (HCC) has a poor prognosis because of its limited drug responses in clinical trials. Therefore, it is crucial to clarify the molecular mechanisms of HCC progression to identify new diagnostic markers and therapeutic targets. Here, we report that brachyury, which regulates the gene encoding the non-SMC condensin II complex subunit G2 (NCAPG2), promotes tumorigenesis in HCC.

Immunotherapy and immunobiomarker in breast cancer: current practice and future perspectives.

Among the new cancer cases and resulting deaths among women worldwide, breast cancer is the most significant threat to women's health. In recent years, immunotherapy was initially used to treat patients with metastatic breast cancer, where it demonstrated its unique value by providing a novel way to improve therapeutic effects and prolong survival time. With the development of clinical trials related to immunotherapy for breast cancer, tumour vaccines, such as DNA vaccines, have been observed to improve the disease-free survival (DFS) and overall survival (OS) of patients.

CDC20 is a novel biomarker for improved clinical predictions in epithelial ovarian cancer.

Epithelial ovarian cancer (EOC), a common tumor of the female reproductive system, ranks first in fatalities among gynecological malignancies. Most patients find tumors at late stage and have extremely poor prognoses, which necessitates improvements in early detection. This study applied bioinformatic methods to identify potential biomarkers of EOC.

Effect of cognitive behavioral intervention on anxiety, depression, and quality of life in patients with epilepsy.

Objective: This study aimed to investigate the effect of cognitive behavioral therapy (CBT) on quality of life, anxiety, and depression in patients with epilepsy.

Methods: Each study subject was randomly assigned to a CBT (n=46) or control (n=49) group (1:1 ratio), and the first group underwent an 8-week CBT treatment. Anxiety, depression, and quality of life (QOLIE-31) were assessed at both baseline and endpoint using the Self-Rating Anxiety Scale (SAS), Hamilton Depression Scale (HDMA) and quality of life in Epilepsy-31 (QOLIE-31) scales.

BMI1 governs the maintenance of mouse GC-2 cells through epigenetic repression of transcription.

Objectives: Studies have demonstrated that B lymphoma Mo-MLV insertion region 1 (BMI1) plays an important role in male reproductive function and the regulation of spermatogonia proliferation. However, whether BMI1 exerts a similarly important function in spermatocyte development remains unclear.

Methods: In this study, we investigated the role of BMI1 in spermatocyte development using a mouse spermatocyte-derived cell line (GC-2) and a -knockout (KO) mouse model.

Effects of high-quality nursing on complications of peripherally inserted central catheter placement in patients with leukemia.

Objective: To determine the effects of high-quality nursing on negative emotions, treatment compliance, and complications of peripherally inserted central catheter (PICC) placement in patients with leukemia.

Methods: A total of 87 patients with leukemia treated in our hospital from May 2018 to May 2020 were retrospectively enrolled and their clinical data were analyzed. The patients were assigned to a routine group (n=40) and a high-quality group (n=47) based on different nursing schemes.

Hydrogel modification of 3D printing hybrid tracheal scaffold to construct an orthotopic transplantation.

Objective: To evaluate the biological properties of modified 3D printing scaffold (PTS) and applied the hybrid graft for transplantation.

Methods: PTS was prepared via 3D printing and modified by Pluronic F-127. Biocompatibility of the scaffold was examined to ascertain its benefit in attachment and proliferation of bone marrow mesenchymal stem cells (BMSCs).

Case report of rescue of a patient with COVID-19 and shock after holmium laser lithotripsy.

Lithotripsy is the primary form of treatment for ureteral calculus. According to clinical data, ureteroscopic lithotripsy (URSL) is characterized by better efficacy, a lower risk of complications, and a quicker postoperative recovery, when compared with open surgery. However, elderly patients often have a variety of chronic diseases that can directly or indirectly influence intraoperative care and postoperative recovery.

Integration of immune and hypoxia gene signatures improves the prediction of radiosensitivity in breast cancer.

Immunity and hypoxia are two important factors that affect the response of cancer patients to radiotherapy. At the same time, considering the limited predictive value of a single predictive model and the uncertainty of grouping patients near the cutoff value, we developed and validated a combined model based on immune- and hypoxia-related gene expression profiles to predict the radiosensitivity of breast cancer patients. This study was based on breast cancer data from The Cancer Genome Atlas (TCGA).

Successful application of PD-1 knockdown CLL-1 CAR-T therapy in two AML patients with post-transplant relapse and failure of anti-CD38 CAR-T cell treatment.

Patients with relapsed/refractory acute myeloid leukemia (R/R AML) often show resistance to chemotherapy and have dismal outcomes. Therefore, it is urgent to develop new treatment strategies to address this problem. With tremendous achievement of chimeric antigen receptor T cells (CAR-T) therapy against B-cell malignancies, many efforts have been devoted to developing CAR-T therapy for R/R AML but with limited success, in part owing to a lack of specific targets.

Efficiency of anti-VEGF therapy in central nervous system AML relapse: A case report and literature review.

There have been few reports on the treatment of central nervous system (CNS) acute myeloid leukemia (AML) relapse. This case study demonstrates that bevacizumab may be a viable treatment option when combined with IT chemotherapy as maintenance therapy for those with CNS leukemia.

Potential effects of COVID-19 on reproductive health: a mini review.

Coronavirus disease 2019 (COVID-19) is now a major public health problem worldwide. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectivity is extremely strong. One major target of the virus is the lung, which can lead to death due to the development of respiratory distress syndrome and even multiple system organ failure.

Fine nursing intervention relieves the clinical symptoms and decreases the adverse events in acute alcoholism patients.

Objective: The purpose of this study is to explore the effect of fine nursing interventions on the clinical efficacy of acute alcoholism patients (AA).

Methods: A total of 100 patients with AA were included in the study. Among them, 51 patients who underwent fine nursing intervention were assigned to the research group (RG), and the remaining 49 patients were treated with routine nursing intervention and were assigned to the control group (CG).

Expression and clinicopathological significance of Foxp3 and VISTA in cervical cancer.

Objective: To detect the expression differences of Foxp3 and VISTA in chronic cervical inflammation, cervical intraepithelial neoplasia, and cervical cancer, and to explore the role of Foxp3 and VISTA in the development of cervical cancer and the effect of Foxp3 and VISTA on the prognosis of cervical cancer, to provide a theoretical basis for clinical immunotherapy of cervical cancer.

Methods: We collected 130 paraffin specimens of cervical tissue, which included 70 cases of cervical cancer tissue, 40 cases of cervical intraepithelial neoplasia tissues and 20 cases of chronic cervicitis. The expression of Foxp3 and VISTA in each group was detected, and the study was conducted based on the clinicopathological characteristics of the patients.

Castleman disease in the scrotum.

Castleman disease (CD) is a rare lymphoproliferative disorder that can affect any lymph node in the body, but CD occurring in the scrotum has not been reported to date. We report the case of a 79-year-old man with a painless hard mass in the right scrotum that has been gradually increasing in size for more than 1 year. Abdominopelvic CT scan showed a heterogeneously enhancing mass of 10 cm long in the right scrotum.

A novel rat tail disc degeneration model induced by static bending and compression.

Background: A new rat tail intervertebral disc degeneration model was established to observe the morphologic and biologic changes of static bending and compression applied to the discs.

Methods: In total, 20 Sprague-Dawley rats with similar weight were randomly divided into 4 groups. Group 1 served as a control group for a baseline assessment of normal discs.

Clinical application of comfort nursing in elderly patients with advanced lung cancer.

Objective: To explore the clinical effect of comfort nursing in elderly patients with advanced lung cancer (ALC).

Methods: Eighty-six elderly patients with ALC treated in our hospital were selected as clinical subjects for this prospective study. The patients were randomly divided into the control group (n=43, received routine nursing) and the observation group (n=43, received additional comfort nursing based on routine nursing) according to a digital table method.

A clinical appraisal of the different types of enteral nutrition support and humanized nursing among cerebral apoplexy ICU patients on mechanical ventilation.

Objective: To study the clinical effectiveness of different types of enteral nutrition support combined with humanized nursing in intensive care unit (ICU) stroke patients on mechanical ventilation.

Methods: One hundred patients with stroke admitted to our hospital's ICU from April 2019 to July 2020 were established as the study cohort and divided into a control group and an experimental group, with 50 cases in each group. The control group was administered one-time injections of enteral nutrition combined with general nursing, and the experimental group was administered enteral nutrition pump infusions combined with humanized nursing.

Effect of comprehensive care on the negative emotions and life quality in parturients with postpartum depression and gestational hypertension.

Objective: To investigate the efficacy of comprehensive care during the nursing process of parturients with gestational hypertension and postpartum depression.

Methods: This prospective study recruited 70 patients with gestational hypertension concurrent with postpartum depression, and the patients were randomly divided into a control group (35 patients received routine care) and an observation group (35 patients, treated by comprehensive care). Indicators including psychological condition, life quality, thyroid function, the incidence of eclampsia, and nursing satisfaction were recorded and compared before intervention (at the diagnosis of postpartum depression) and after intervention (three months after delivery) in the two groups.

Microfluidic synthesis of high-valence programmable atom-like nanoparticles for reliable sensing.

Synthesis of programmable atom-like nanoparticles (PANs) with high valences and high yields remains a grand challenge. Here, a novel synthetic strategy of microfluidic galvanic displacement (μ-GD) coupled with microfluidic DNA nanoassembly is advanced for synthesis of single-stranded DNA encoder (SSE)-encoded PANs for reliable surface-enhanced Raman scattering (SERS) sensing. Notably, PANs with high valences (, -valence, = 12) are synthesized with high yields (, >80%) owing to the effective control of interfacial reactions sequentially occurring in the microfluidic system.

Timing of continuous renal replacement therapy in patients with acute non-ST-segment elevation myocardial infarction complicated with cardiac and renal insufficiency.

Objective: To explore the efficacy of different timing options for continuous renal replacement therapy (CRRT) in patients with acute non-ST-segment elevation myocardial infarction (NSTEMI) and cardiac and renal insufficiency.

Methods: Eighty-eight patients with acute NSTEMI complicated with cardiac and renal insufficiency received PCI treatment after achieving a stable condition and were randomly divided into the control group (n = 44) and the research group (n = 44). The control group was given CRRT after percutaneous coronary intervention (PCI), and the research group was treated with CRRT before and after PCI.