Construction of a coumarin-based fluorescent probe for accurately visualizing hydrogen sulfide in live cells and zebrafish.

Hydrogen sulfide (HS), an important gas signaling molecule, is a regulator of many physiological processes, and its abnormal levels are closely related to the onset and progression of disease. It is vital to develop methods for specific tracking of HS in clinical diagnosis and treatment. In this study, we designed an ultrasensitive and highly stable coumarin-based fluorescent probe Cou-HS.

Prognostic prediction of thrombolytic therapy with rt-PA in acute ischemic stroke patients using an artificial intelligence model.

Objective: Through the comparison of different prediction models, we hope to find a promising statistical method to evaluate the prognosis of patients with acute ischemic stroke (AIS) after thrombolytic therapy.

Methods: Data of 518 patients who received thrombolytic therapy were retrospectively collected in this study. Among them, 362 patients met the eligibility criteria, so their data such as age, sex, smoking history, previous medical history, clinical and laboratory indicators were analyzed.

Presence of tumor deposits is an indicator of poor prognosis in patients with pancreatic ductal adenocarcinoma.

Tumor deposits (TDs) are associated with poor prognosis in several malignancies and have been incorporated into the tumor-node-metastasis (TNM) staging system for colorectal cancer. This study aims to explore the significance of TDs in pancreatic ductal adenocarcinoma (PDAC). All patients who underwent pancreatectomy with a curative intent for PDAC were retrospectively enrolled.

Engineering exposed vertical nano-TiO (001) facets/BiOI nanosheet heterojunction film for constructing a satisfactory PEC glucose oxidase biosensor.

In the field of photoelectrochemical (PEC) enzyme biosensors, constructing efficient photoelectrodes, in which the recombination of photogenerated carriers is an important factor affecting the performance, is of great significance. Herein, to enhance the separation efficiency of photogenerated carriers, titanium dioxide (TiO) nanosheet (NS)/bismuth oxyiodide (BiOI) NS/glucose oxidase (GOx) composites were prepared hydrothermal and solvothermal methods. Single-crystal anatase TiO NSs with a high percentage of (001) facets lead to better photocarrier separation due to heterojunctions between facets.

Ultrasonic characteristics and influencing factors of atherosclerosis in diabetic patients.

Objective: The purpose of this research was to observe the characteristics of atherosclerosis in diabetic patients by ultrasound and analyze the factors influencing the development of atherosclerosis in these patients.

Methods: Ninety diabetic patients treated in our hospital from January 2019 to December 2019 were enrolled in this retrospective analysis. The transcranial Doppler ultrasound (TCD) and carotid ultrasound were used to determine the presence of intracranial (stenosis) and extracranial (plaque) atherosclerosis.

Factors influencing quality of life and work of male nurses in Hainan Province: logistic regression analysis.

Objective: The purpose of this study was to explore the factors affecting the quality of life and work of male nurses in Hainan Province, so as to provide a theoretical basis to improve the motivation of male nurses, reduce job-hopping intentions, and maintain the stability of labor force.

Methods: A total of 393 male and 393 female nurses with registered nurse qualifications from 53 hospitals in Hainan Province were selected as the research subjects. The distribution in various hospital departments, quality of life, stress coping ability, social support and job satisfaction were compared.

Sensitive and effective imaging of carbon monoxide in living systems with a near-infrared fluorescent probe.

CO, a gas molecule that is harmful to living organisms, has a high affinity with hemoglobin, which will cause severe hypoxia. However, in recent years, researchers have discovered that endogenous CO, similar to NO, is one of the messenger molecules, which has a certain regulatory effect in many physiological and pathological processes in the respiratory system, cardiovascular system, and nervous system. Therefore, it is urgent to explore an effective method to monitor the role of CO under physiological and pathological conditions.

Development of an ultrafast fluorescent probe for specific recognition of hypochlorous acid and its application in live cells.

Hypochlorous acid (HOCl), a highly potent oxidant of reactive oxygen species, plays critical roles in many physiological and pathological processes. In this work, a novel coumarin-based fluorescent probe, Cou-HOCl, was prepared for the detection of HOCl. The probe exhibited good selectivity over other analytes, excellent sensitivity with a detection limit of 16 nM, and fast response within 5 s.

A dual responsive fluorescent probe for selective detection of cysteine and bisulfite and its application in bioimaging.

A coumarin-based dual responsive fluorescent probe with a simple structure was developed for the detection of Cys and HSO . Under simulated physiological conditions, Cou-F displayed an on-off fluorescence response to Cys at 521 nm and an off-on fluorescence response to HSO at 500 nm. Furthermore, Cou-F had the advantages of high sensitivity, strong specificity and rapid response.

The influence of comprehensive nursing intervention on the compliance of glaucoma patients with their doctors' advice.

Objective: To determine the effect of targeted comprehensive nursing on the compliance of glaucoma patients with their doctors' advice.

Methods: A total of 78 patients with glaucoma admitted to the Ophthalmology Department of the First Affiliated Hospital of Hainan Medical University were retrospectively enrolled and assigned to a routine nursing group and a comprehensive nursing group according to the different nursing modes each patient underwent. The routine nursing group underwent routine nursing, and the comprehensive nursing group underwent comprehensive nursing intervention.

Pathologic role of peptidyl-prolyl isomerase Pin1 in pulmonary artery remodeling.

Peptidyl-prolyl isomerase Pin1 is crucial for cell proliferation, but its role in pulmonary artery remodeling (PAR) is unclear. In the present study, we aimed to evaluate the expression and contribution of Pin1 in PAR. Treatment with Pin1 inhibitor Juglone or Pin1-specific siRNAs ameliorated the expression of Pin1 and proliferating cell nuclear antigen (PCNA) in human pulmonary artery smooth muscle cells (PASMCs) , and Juglone treatment arrested the cell cycle at the G1 phase.

Nursing effect of continuous blood purification therapy in treatment of severe sepsis.

Objective: To explore the nursing effect of continuous blood purification therapy in the treatment of severe sepsis patients.

Methods: A total of 142 patients with severe sepsis in our hospital were divided into two groups, 70 patients in the experimental group who received an optimize nursing plan, while 72 patients in the control group were given routine nursing intervention. The SF-36 questionnaire, nursing satisfaction and serious adverse events and complications were collected.

Failure mode and effects analysis on the control effect of multi-drug-resistant bacteria in ICU patients.

Objective: The failure mode and effect analysis of the prevention and control in intensive care unit (ICU) patients with multi-drug-resistant (MDR) bacterial infection were explored and analyzed in this research.

Methods: A total of 251 critically ill patients who were hospitalized in the ICU from June to December 2019 were selected as the control group, and another 258 patients who were hospitalized in the ICU from January to June 2020 were set as the observation group. The control-group patients received conventional ICU care, the observation group was treated by the failure mode and effects analysis (FMEA), and then the prevention and control effect of the two nursing modes on multi-drug-resistant bacteria infection in the two groups were compared accordingly.

Nursing effect of Nasoscopically assisted nasogastri tube and nasojejunal tube placement.

Objective: To investigate the nursing effect of nasoscopically assisted nasogastri tube and nasojejunal tube placement.

Methods: 94 patients who need to place nasogastric tube and nasojejunal tube to establish enteral nutrition were randomly divided into two groups: the observation group (n=49) and control group (n=45). The patients in the observation group received nasogastric tube placement and jejunal nutrition tube placement, and the patients in the control group received general gastroscope and placed gastric tube and jejunal nutrition tube through mouth.

A study of the clinical application value of ultrasound and electrocardiogram in the differential diagnosis of cardiomyopathy.

Objective: To investigate the clinical application values of electrocardiogram (ECG) combined with ultrasound cardiogram (UCG) in the differential diagnosis of cardiomyopathy.

Methods: A total of 120 patients with cardiomyopathy who were admitted to our hospital were randomly divided into the dilated cardiomyopathy (DCM) group (60 cases) and the ischemic cardiomyopathy (ICM) group (60 cases). Both groups were examined using ECG combined with UCG.