1 results match your criteria: "The First Affiliated Hospital of Chongqing Medical University No. 1 Medical College Road[Affiliation]"
Scutellarin inhibits glioma cell proliferation by up-regulating miR-15a expression.
Am J Transl Res
March 2023
Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University No. 1 Medical College Road, Yuzhong District, Chongqing 400016, China.
Objective: To investigate the effect of scutellarin on the proliferation of glioma cells through microRNA (miR)-15a.
Methods: Human glioma cell line T98G was cultured in vitro and divided into control group (without treatment), scutellarin group (with 10, 20, 40, 80, 160 μg/mL scutellarin, respectively), miR-15a negative control group (transfected with negative control-miR-15a + 80 μg/mL scutellarin) and miR-15a inhibitor group (transfected with miR-15a siRNA + 80 μg/mL scutellarin). The proliferation of T98G cells was detected by cell counting kit-8 (CCK-8), and the expression of miR-15a in T98G cells was detected by real-time fluorescence quantitative PCR (qRT-PCR).