The Effectiveness of Digital Animation-Based Multistage Education for Patients With Atrial Fibrillation Catheter Ablation: Randomized Clinical Trial.

Background: Digital education for outpatient patients with atrial fibrillation (AF) has gradually increased. However, research on digital education for patients undergoing atrial fibrillation catheter ablation (AFCA) is limited.

Objective: This study aimed to develop a novel digital animation-based multistage education system and evaluate its quality-of-life benefits for patients with AFCA.

Predictive value of magnetic resonance imaging parameters combined with tumor markers for rectal cancer recurrence risk after surgery.

Background: An increasing number of studies to date have found preoperative magnetic resonance imaging (MRI) features valuable in predicting the prognosis of rectal cancer (RC). However, research is still lacking on the correlation between preoperative MRI features and the risk of recurrence after radical resection of RC, urgently necessitating further in-depth exploration.

Aim: To investigate the correlation between preoperative MRI parameters and the risk of recurrence after radical resection of RC to provide an effective tool for predicting postoperative recurrence.

Clipping of anterior circulation aneurysms using fully endoscopic-assisted minimally invasive keyhole craniotomy: a clinical study and analysis.

Endoscopy's ability to provide close observation, deep magnification, and multi-angle views has proven to be an effective tool for minimally invasive craniotomy in neurosurgery. However, no large case series have been published on the use of fully endoscopic-assisted minimally invasive keyhole craniotomy for clipping intracranial aneurysms (IAs). To evaluate the value of fully endoscopic-assisted minimally invasive keyhole craniotomy in the treatment of anterior circulation aneurysms.

Measurement of axial dose profile for wide detector CT using radiochromic films.

To investigate the application of radiochromic film LD-V1 for measuring the dose profile of wide detector CT and comparing the measurements with the console-displayed CTDI, with the aim of obtaining correction factors. Calibration curves in CTDI phantoms were first obtained with various tube currents at 120 kV. Then, at the selected wide beam collimations (80, 100, 120, 140, 160 mm), the film was placed in the extended phantom holes to obtain the dose profile.

Lactate released by lung adenocarcinoma (LUAD) cells promotes M2 macrophage polarization via the GPR132/cAMP/PKA pathway.

Background: Increasing evidence suggests that lactate is an essential compound in the tumor microenvironment, and especially for macrophage cells. However, the mechanism by which lactate affects macrophages remains unclear.

Objective: This study investigated whether and how lactate affects macrophage polarization in lung adenocarcinoma (LUAD).

Impacts of sarcopenia on adverse events and prognosis in Chinese patients with esophageal cancer undergoing chemoradiotherapy.

Background: Sarcopenia is a common indicator of systemic nutritional status in patients with cancer progression. This study investigated the impacts of sarcopenia on adverse effects and prognosis of sarcopenia on patients with esophageal cancer receiving chemoradiotherapy.

Methods: The clinical data of 158 patients with initially diagnosed esophageal cancer who received chemoradiotherapy were collected, and nutritional indexes and inflammatory markers were calculated.

Paeoniflorin modulates AGEs/RAGE/P38MAPK/ERK/mTOR autophagy pathway to improve cognitive dysfunction in MRL/lpr mice.

Objective: The objective of this study was to investigate the therapeutic effects of paeoniflorin (PA) on cognitive impairment and to elucidate its potential mechanisms in MRL/lpr mice, a model of systemic lupus erythematosus-associated cognitive dysfunction.

Method: Cognitive performance and behavioral responses were assessed using a comprehensive battery of tests, including the Morris water maze, the Novel object recognition test, and the Y maze. Neuropathological changes in the hippocampal regions were visualized through Nissl, HE and Immunohistochemistry staining.

Sensitive organelles of U251 MG glioblastomas to boron neutron capture therapy.

Purpose: The micro-distribution of boron compounds within cells influences the effectiveness of boron neutron capture therapy (BNCT) in killing tumor cells. Boron neutron capture therapy-sensitive organelles within the range of α particles and Li recoil nuclei can enhance killing effects on tumor cells. However, comprehensive studies on the sensitive organelles to BNCT are currently lacking.

New options for cilostazol-based dual antiplatelet therapy for ischaemic stroke prevention in East Asian populations: a systematic review and meta-analysis.

Background And Purpose: The objective of this study is to systematically review the efficacy and safety of cilostazol-based dual antiplatelet therapy (DAPT) in patients with stroke.

Methods: Two reviewers conducted a comprehensive search of eligible studies published in PubMed, Medline, the Cochrane Library, Embase, and four Chinese databases from their establishment to 31 July 2024. The review was registered (CRD42024559047).

LINC01871 facilitates cervical cancer cell migration and immune escape by targeting miR-873-3p/MAP3K2 axis.

Cervical cancer (CC) poses a significant threat to women's health and lives worldwide. Emerging evidence indicates that long noncoding RNA LINC01871 is closely associated with immune regulation in CC patients. However, its specific role and underlying mechanisms in CC remain poorly understood.

17β-estradiol delays cardiac aging through suppressing the methylation of Beclin1 in a murine model.

Introduction: Cardiac endogenous senescence will gradually change and aggravate with age. Recent research showed that 17β-estradiol (17β-E2), an estrogen with numerous biological activities including the prevention of vascular senescence. However, how 17β-E2 against cardiac aging is still unknown.

IRF1 is a core transcriptional regulatory circuitry member promoting AML progression by regulating lipid metabolism.

Background: Acute myeloid leukemia (AML) is a prevalent malignancy of the hematologic system. Despite advancements in therapeutic approaches, significant heterogeneity and therapeutic resistance pose substantial challenges to treatment. Tumors driven by core transcription factors through super-enhancers can establish core transcriptional regulatory circuits (CRCs) that modulate oncogene expression programs.

Histone lactylation protects against sevoflurane-induced cognitive impairment by regulating YTHDF3/PRDX3 mediated microglial pyroptosis in neonatal mice.

Background: Sevoflurane (SEV), a widely used inhalational anesthetic, multiple SEV exposures has been implicated in cognitive impairment, particularly in neonates. However, the mechanisms underlying SEV-induced cognitive impairment not fully understood.

Methods: Neonatal mice or murine microglial line BV-2 cells were exposed to SEV.

Immunosenescence and immunotherapy in elderly patients with hepatocellular carcinoma.

Liver cancer, more specifically hepatocellular carcinoma (HCC), is a global health issue and one of the dominant causes of cancer death around the world. In the past few decades, remarkable advances have been achieved in the systemic therapy of HCC. Immune checkpoint inhibitors (ICIs) have become a therapy mainstay for advanced HCC and have shown promise in the neoadjuvant therapy before resection.

Adipose-Derived Stem Cell Exosomes Promote Scar-Free Healing of Diabetic Wounds via miR-204-5p/TGF-1/Smad Pathway.

Numerous researches have demonstrated the therapeutic potential of adipose-derived stem cell exosomes (ADSC-Exos) in promoting wound healing. In this study, we aimed to investigate the impact of ADSC-Exos on diabetic wound fibroblasts and elucidate its possible mechanisms. CCK-8, Edu, cell scratch, and Transwell tests were used to evaluate the function of ADSC-Exos on rat skin fibroblasts (RSFs) in high-glucose (HG) medium.

[Recent Advances in Diagnosis and Treatment of Diffuse Alveolar Hemorrhage after Hematopoietic Stem Cell Transplantation --Review].

Diffuse alveolar hemorrhage (DAH) is a severe complication that can occur post- hematopoietic stem cell transplantation (HSCT). So far, the precise pathogenesis and risk factors of DAH post-HSCT remain elusive, diagnostic criteria have not reached a consensus, and the efficacy of existing therapeutic measures is far from satisfactory. At present, it is believed that the core mechanism of DAH post-HSCT is a vicious cycle initiated by endothelial injury, accompanied by a series of subsequent inflammatory and cellular responses.

[Efficacy and Safety of BeEAM, a Conditioning Regimen for Autologous Stem Cell Transplantation in Malignant Lymphoma].

Objective: To investigate the efficacy and safety of the conditioning regimen BeEAM (bendamustine+et-oposide+cytarabine+melphalan) in autologous stem cell transplantation (ASCT) for patients with malignant lymphoma.

Methods: The clinical data of 20 patients with malignant lymphoma who underwent ASCT after conditioning with BeEAM regimen from January 2021 to December 2022 in the First Affiliated Hospital of Bengbu Medical University were collected, and the clinical characteristics before transplantation, conditioning-related toxicity, hematopoietic reconstitution after transplantation, and therapeutic effects were analyzed. 67 patients with malignant lymphoma who did not undergo ASCT during the same period were selected as the control group, and the 1-year progression-free survival (PFS) rate and overall-survival (OS) rate between the ASCT group and the non-ASCT group were compared.

Multi-parametric MRI Habitat Radiomics Based on Interpretable Machine Learning for Preoperative Assessment of Microsatellite Instability in Rectal Cancer.

Rationale And Objectives: This study constructed an interpretable machine learning model based on multi-parameter MRI sub-region habitat radiomics and clinicopathological features, aiming to preoperatively evaluate the microsatellite instability (MSI) status of rectal cancer (RC) patients.

Materials And Methods: This retrospective study recruited 291 rectal cancer patients with pathologically confirmed MSI status and randomly divided them into a training cohort and a testing cohort at a ratio of 8:2. First, the K-means method was used for cluster analysis of tumor voxels, and sub-region radiomics features and classical radiomics features were respectively extracted from multi-parameter MRI sequences.

Mechanism of lncRNA ZFAS1 mediating nucleus pulposus cell pyroptosis in intervertebral disc degeneration.

Background: This study investigates the mechanism of lncRNA ZFAS1 in pyroptosis of TNF-α-induced nucleus pulposus cells (NPCs) in intervertebral disc degeneration (IDD).

Methods: Mouse NPCs were isolated and induced by TNF-α to establish a cell model of IDD. The cell viability was evaluated by MTT assay.

HIF-1α Enhances Intestinal Injury and Inflammation in Severe Acute Pancreatitis Through NLRP3 Inflammasome Activation.

Background: Severe Acute Pancreatitis (SAP) is associated with significant intestinal injury and inflammation. Hypoxia-Inducible Factor-1α (HIF-1α) and NLRP3 inflammasome have been implicated in this process, but their specific roles remain unclear.

Objective: This study aims to elucidate the roles of HIF-1α and NLRP3 in the pathogenesis of SAP and their effects on intestinal injury, barrier function, and inflammatory responses.

BHPF inhibits early embryonic development in mice by disrupting maternal-to-zygotic transition and mitochondrial function.

Fluorene-9-bisphenol (BHPF), a prevalent substitute for bisphenol A (BPA), has become a widespread endocrine disruptor found in numerous consumer products. Despite extensive research on its toxicological profile, the specific effects of BHPF on reproduction, particularly during early embryonic development, remain unclear. Therefore, in our study, we used an in vitro culture system of mouse embryos to treat fertilized eggs with different concentrations of BHPF, and applied immunofluorescence, cell live staining and transcriptome sequencing to explore the effects of BHPF on early embryonic development and related mechanisms.

MiR-224-3p regulates ferroptosis and inflammation in lens epithelial cells by targeting ACSL4.

In this study, we investigated the expression levels of miR-224-3p and inflammatory factors in the lens epithelium of patients with high myopia cataract (HMC) to determine how miR-224-3p/ACSL4 affects ferroptosis and inflammation in human lens epithelial cells (HLECs). The capsule tissues (including lens epithelial cells) of 36 patients with HMC and 36 patients with age-related cataract (ARC) were taken respectively. 18HMC and 18ARC capsule tissues were selected for RNA sequencing (RNA-Seq) and the rest were used for qPCR assays.

Single-cell programmed cell death regulator patterns guide intercellular communication of cancer-associated fibroblasts that contribute to colorectal cancer progression.

Background: The significance of programmed cell death (PCD) in the context of cancer development and progression is widely acknowledged, yet its specific impact on cancer-associated fibroblasts (CAFs) remains a topic of ongoing investigation. Therefore, the study aims to explore the role of PCD in regulating CAFs and its potential implications for CRC progression.

Methods: CAFs from single-cell data of 23 colorectal cancer (CRC) patients were clustered by non-negative matrix factorization (NMF) and the impact of these subpopulations on the prognosis of CRC patients was predicted using public database cohorts.

Angiotensin II and AGTR1 in ESCC Proliferation and Invasion: Insights from the JAK/STAT3 Pathway.

Background: Angiotensin II (Ang II) and its receptor, Angiotensin II receptor type 1 (AGTR1), have been implicated in the proliferation of cancer cells across various tumor types. This study aims to examine the impact of Ang II and AGTR1 on esophageal squamous cell carcinoma (ESCC) cells.

Methods: The clonogenicity and proliferation of tumor cells were evaluated through Clone Formation and Cell Counting Kit-8 (CCK-8) assays.