Extracellular DNA: A Missing Link in the Pathogenesis of Ectopic Mineralization.

Although deoxyribonucleic acid (DNA) is the genetic coding for the very essence of life, these macromolecules or components thereof are not necessarily lost after a cell dies. There appears to be a link between extracellular DNA and biomineralization. Here the authors demonstrate that extracellular DNA functions as an initiator of collagen intrafibrillar mineralization.

Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique.

Objective: To evaluate the mechanical stability and the proteolytic activity of bonds created by a two-step, etch-and-rinse adhesive applied to cross-linked and air-dried etched dentin.

Methods: Flat dentin surfaces were produced in 64 extracted sound human molars. The dentin was etched with 35% phosphoric acid for 15 seconds, and then the teeth were divided into groups according to the cross-linking solution applied on the etched dentin.

Efficacy and safety of a novel mucoadhesive clobetasol patch for treatment of erosive oral lichen planus: A phase 2 randomized clinical trial.

Background: Oral lichen planus (OLP) is a chronic inflammatory disorder of the oral mucosa. Currently there is no approved treatment for OLP. We report on the efficacy and safety of a novel mucoadhesive clobetasol patch (Rivelin -CLO) for the treatment of OLP.

The root submergence technique at single tooth sites to short-span edentulous sites: A step-by-step partial extraction therapy protocol.

Submerging roots for ridge preservation was the earliest partial extraction therapy and has been described since the 1970s. Despite the approximately 47 animal and human studies published since, an updated and contemporary step-by-step protocol has not yet been provided. This technique report describes in detail how to manage submerged roots at single tooth sites and at short-span edentulous areas.

Proteolytic activity and degradation of bovine versus human dentin matrices.

Objective: Non-human teeth have been commonly used in research as replacements for human teeth, and potential dissimilarities between the dental tissues should be considered when interpreting the outcomes. To compare the proteolytic activity and degradation rate of bovine and human dentin matrices.

Methodology: Dentin beam specimens were obtained from human molars (n=30) and bovine incisors (n=30).

Multifunctional Nanomachinery for Enhancement of Bone Healing.

The visionary idea that RNA adopts nonbiological roles in today's nanomaterial world has been nothing short of phenomenal. These RNA molecules have ample chemical functionality and self-assemble to form distinct nanostructures in response to external stimuli. They may be combined with inorganic materials to produce nanomachines that carry cargo to a target site in a controlled manner and respond dynamically to environmental changes.

Dentin Matrix Protein 1 on Titanium Surface Facilitates Osteogenic Differentiation of Stem Cells.

Dentin matrix protein 1 (DMP1) contains a large number of acidic domains, multiple phosphorylation sites, a functional arginine-glycine-aspartate (RGD) motif, and a DNA binding domain, and has been shown to play essential regulatory function in dentin and bone mineralization. DMP1 could also orchestrate bone matrix formation, but the ability of DMP1 on Ti to human mesenchymal stem cell (hMSC) conversion to osteoblasts has not been studied. There is importance to test if the DMP1 coated Ti surface would promote cell migration and attachment to the metal surface and promote the differentiation of the attached stem cells to an osteogenic lineage.

Quo vadis, esthetic dentistry? Ceramic veneers and overtreatment-A cautionary tale.

The increased emphasis on orofacial esthetics, experienced both by dental professionals and the lay public, results in an environment where overtreatment can easily occur. Patients on the one hand feel pressure from esthetic norms that are often unrealistic, while dental professionals are compelled to deliver immediate results many times without considering what is best for the ill-informed patient. This article is an illustrated cautionary tale against overtreatment disguised as esthetic dentistry.

A mussel glue-inspired monomer-etchant cocktail for improving dentine bonding.

Objectives: The humid oral environment adversely affects the interaction between a functionalised primer and dentine collagen after acid-etching. Robust adhesion of marine mussels to their wet substrates instigates the quest for a strategy that improves the longevity of resin-dentine bonds. In the present study, an etching strategy based on the incorporation of biomimetic dopamine methacrylamide (DMA) as a functionalised primer into phosphoric acid etchant was developed.

Osteosarcoma of the jaws: Report of 3 cases with emphasis on the early clinical and radiographic signs.

Osteosarcoma is the most common primary malignancy of the jaws and is treated by radical surgical resection. Early detection is crucial because removing the lesion with clean margins contributes most to the outcome. We present 3 cases of osteosarcoma occurring in the interradicular region of the mandible, 2 of which were thought to represent nonmalignant processes upon initial presentation.

Micro-computed Tomographic Evaluation of the Quality of Root Canal Fillings in Mandibular Molars after Obturation for 54 Months.

Introduction: The present study evaluated the percentage volume of voids in root canals of mandibular molars that had been obturated for 54 months.

Methods: Thirty extracted human mandibular molars were instrumented and debrided. The teeth were assigned to 3 groups (n = 10) according to the filling technique and sealer used: the single-cone technique using AH Plus sealer (AHS; Dentsply Sirona Endodontics, Tulsa, OK) or EndoSequence BC sealer (BCS; Brasseler USA Dental LLC, Savannah, GA) and the warm vertical compaction technique using AH Plus sealer (AHW).

Evaluation of the golden proportion in the natural dentition: A systematic review and meta-analysis.

Statement Of Problem: Different proportions of tooth width ratios have been evaluated, and the golden proportion is the best known. Although the prevalence of the golden proportion has been evaluated in different ethnic groups and populations, a meta-analysis of these studies is lacking.

Purpose: The purpose of this systematic review and meta-analysis was to investigate the existence and suitability of the golden proportion for tooth width ratios and to assess the prevalence of the golden proportion among populations.

Effects of dentine surface cleaning on bonding of a self-etch adhesive to root canal sealer-contaminated dentine.

Objectives: To compare the effectiveness of different sealer cleaning methods on bonding of a self-etch adhesive to root canal sealer-contaminated dentine.

Methods: Forty-nine extracted molars were divided into seven groups. Dentine surfaces were exposed and cotaminated with a thin layer of epoxy resin-based sealer (AH Plus (AHP)) or zinc oxide eugenol-based sealer (Pulp Canal Sealer (PCS)).

Endogenous Enzymatic Activity of Primary and Permanent Dentine.

Matrix metalloproteinases (MMPs) play an important role in tooth development and influence caries development and hybrid layer degradation. Literature is scant on the differences in the activity of MMPs between primary and permanent dentine. Accordingly, the aim of the present study was to investigate endogenous gelatinolytic activity in primary and permanent dentine.

Long-term survival and success of zirconia screw-retained implant-supported prostheses for up to 12 years: A retrospective multicenter study.

Statement Of Problem: Despite the broad clinical application of zirconia for fixed implant-supported prostheses, evidence of long-term performance is sparse.

Purpose: The purpose of this retrospective study was to evaluate the long-term clinical and radiographic outcomes of zirconia-based partial and complete screw-retained implant-supported zirconia fixed dental prostheses (ISZFDPs).

Material And Methods: Records of patients treated with dental implants and ISZFDPs between December 2004 and June 2017 were screened.

Efficacy of XP-endo instruments in removing 54 month-aged root canal filling material from mandibular molars.

Objective: To investigate the effectiveness of XP-endo Shaper (XPS) and XP-endo Finisher R (XPFR) instruments in removing aged root filling material from root-treated mandibular molars.

Methods: Thirty mandibular molars were instrumented and divided into three groups: single-cone obturation using AH Plus sealer (AHS) or EndoSequence BC sealer (BCS), and warm vertical compaction using AH Plus sealer (AHW). The specimens were stored at 100% humidity and 37 °C for 54 months.

Bleaching: the initial treatment consideration for tetracycline-stained teeth.

Tray tooth bleaching involves the use of carbamide peroxide in a custom-fitted tray to bleach teeth. One of the most difficult stains to bleach is tetracycline. This paper will present several different patient situations of tetracycline-stained teeth being bleached and will discuss the benefits and limitations of bleaching tetracycline-stained teeth.