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Acceleration of bone-defect repair by using A-W MGC loaded with BMP2 and triple point-mutant HIF1α-expressing BMSCs.

J Orthop Surg Res

May 2015

Department of Orthopedics, The Affiliated First Hospital of China Medical University, No. 155, Nanjing North Street, Heping District, 110001, Shenyang, Liaoning province, China.

Background: The goal of this study is to explore the effects of A-W MGC (apatite-wollastonite magnetic bioactive glass-ceramic) loaded with BMP2 (bone morphogenetic protein 2)- and HIF1α(mu) (hypoxia-inducible factor 1 mutation)-expressing BMSCs (bone marrow mesenchymal stem cells) on the bone defect repair.

Methods: (1) BMSCs were infected with viral solution containing BMP2 and HIF1α(mu) with the best MOI (multiplicity of infection). The efficiency was observed via hrGFP (human renilla reniformis green fluorescent protein).

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