51 results match your criteria: "The ADA Forsyth Institute; Cambridge[Affiliation]"

The dynamic oral-gastric microbial axis connects oral and gastric health: current evidence and disputes.

NPJ Biofilms Microbiomes

January 2025

State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Frontier Innovation Center for Dental Medicine Plus & Department of Preventive Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.

Emerging evidence indicates that oral microbes are closely related to gastric microbes and gastric lesions, including gastric atrophy, intestinal metaplasia and gastric cancer (GC). Helicobacter pylori is a key pathogen involved in GC. However, the increasing prevalence of H.

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Engineered as Oral Probiotics To Enhance Clearance of Blood Lactate.

ACS Synth Biol

December 2024

Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, United States.

Article Synopsis
  • Elevated lactate levels are linked to serious health issues, including sepsis and mitochondrial dysfunction, and poor lactate clearance can lead to worse outcomes in these conditions.
  • Current methods for managing elevated lactate are limited, but recent findings highlight the gut's role in lactate regulation, suggesting a potential link between gut bacteria and blood lactate levels.
  • This study presents a promising approach using engineered probiotic spores to deliver lactate oxidase to the gut, which successfully lowers blood lactate in mice without harming gut health or immune function.
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Tooth enamel maturation requires the removal of proteins from the mineralizing enamel matrix to allow for crystallite growth until full hardness is reached to meet the mechanical needs of mastication. While this process takes up to several years in humans before the tooth erupts, it is greatly accelerated in the faster-developing pigs. Pig teeth erupt with softer, protein-rich enamel that is similar to hypomineralized human enamel but continues to harden quickly after eruption.

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Cancerous tissue is a largely unexplored microbial niche that provides a unique environment for the colonization and growth of specific bacterial communities, and with it, the opportunity to identify novel bacterial species. Here, we report distinct features of a novel species, sp. nov.

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Background: A stable copper tetraamine fluoride (CTF) with low cytotoxicity has been developed for dental use.

Objective: To investigate the antimicrobial effects of CTF against common microbes associated with dental caries and periodontal disease.

Method: The minimum inhibitory concentrations (MIC) and minimum bactericidal/fungicidal concentrations (MBC/MFC) were used to evaluate the antimicrobial effects of CTF against eight common bacteria and one fungus associated with dental caries and periodontal disease.

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All cultivated Patescibacteria, or CPR, exist as obligate episymbionts on other microbes. Despite being ubiquitous in mammals and environmentally, molecular mechanisms of host identification and binding amongst ultrasmall bacterial episymbionts are largely unknown. Type 4 pili (T4P) are well conserved in this group and predicted to facilitate symbiotic interactions.

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strain BB002, was isolated from the human oral cavity on its basibiont bacterial host sp. oral taxon 171 strain F0337, related to . As a member of the within the candidate phylum radiation group (CPR), its reduced genome facilitates the survival as an ultrasmall (<0.

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We developed a translational prototype antimicrobial blue light (ABL) device for treating skin wounds with ABL. Partial-thickness surgical wounds were created in live swine, an animal whose skin is considered the most like human skin, then heavily contaminated and left untreated for 24 hours with methicillin-resistant Staphylococcus aureus (MRSA). ABL treatment stabilized and reduced MRSA infection by greater than four orders of magnitude (>99.

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Objective: To investigate the antibacterial, remineralising, and discolouring effects of copper tetraamine fluoride (CTF) on artificial enamel caries.

Method: Human enamel blocks with artificial caries were treated with CTF, silver diamine fluoride (SDF, positive control) and water (negative control) before being challenged with Streptococcus mutans. The morphology, viability, and growth kinetics of biofilm were evaluated using scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and colony-forming unit (CFU) counting.

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Objective: To investigate the antibacterial, mineralising, and discolouring properties of copper tetraamine fluoride (CTF) on artificial dentine caries.

Method: Demineralised human dentine blocks were treated with CTF, silver diamine fluoride (SDF, positive control) and water (negative control) before they were subjected to cariogenic challenge with Streptococcus mutans biofilm. The morphology, viability, and growth kinetics of the biofilm were assessed by scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and colony-forming unit (CFU) counting.

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The necessity for regenerating peri-implant and periodontal tissues is increasingly apparent. Periodontal diseases can result in a significant loss of clinical attachment level, and tissue regeneration stands as the ultimate goal of periodontal therapy. With the rise of osseointegration, the prosthetic rehabilitation of missing teeth using dental implants has surged, leading to a frequent need for alveolar bone regeneration around implants.

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Article Synopsis
  • - Craniofacial and alveolar bone defects from various causes (like tumors, trauma, or tooth loss) present significant challenges in tissue engineering and dental surgery, especially in cases of periodontal disease.
  • - Specialized pro-resolving lipid mediators (SPMs) like lipoxins, resolvins, protectins, and maresins are being researched for their potential to enhance bone regeneration and restore tissue healing in craniofacial applications.
  • - A total of 19 pre-clinical studies examined the effects of SPMs on bone regeneration, showing that SPMs notably increased new bone formation by about 14.85% and reduced defect size, with RvE1 and RvD2 demonstrating
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Soluble epoxide hydrolase inhibition impairs triggering receptor expressed on myeloid cells-1 in periodontal tissue.

J Periodontal Res

September 2024

Laboratory of Neuroimmune Interface of Pain Research, Faculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, Brazil.

Aims: Periodontitis is a prevalent inflammatory disorder affecting the oral cavity, driven by dysbiotic oral biofilm and host immune response interactions. While the major clinical focus of periodontitis treatment is currently controlling oral biofilm, understanding the immune response is crucial to prevent disease progression. Soluble epoxide hydrolase (sEH) inhibition has shown promise in preventing alveolar bone resorption.

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Article Synopsis
  • - This study explored the rising interest in using copper materials for managing dental caries, examining 81 relevant publications through extensive literature searches and bibliometric analysis.
  • - The majority of the studies focused on copper or copper oxide nanoparticles, which can be applied in dental products like topical agents, adhesives, and implants.
  • - Publication and citation data indicate significant growth in research from 2019 to 2024, with key topics identified as nanoparticles and antibacterial activity, particularly highlighting the antimicrobial effects of copper oxide nanoparticles.
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Background: Behçet's disease (BD) pathogenesis involves severe outcomes such as blindness, central nervous system manifestations, and deep venous thrombosis that impacts systemic and local inflammatory changes. We tested the hypothesis that BD negatively affects gingival health and increases the severity of gingivitis.

Methods: The study included 37 BD patients with gingivitis without any sign of periodontitis.

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Sjӧgren's syndrome (SS), also known as Sjögren's disease, is a chronic autoimmune condition predominantly affecting the salivary and lacrimal glands. The disease is driven by autoimmune responses involving the activation and actions of major innate- and adaptive immune cell subsets. However, the specific characteristics and roles of regulatory T cells (Tregs) in SS remain elusive.

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In-house 3D-printed shape memory aligners for retreatment after fixed retainer failure.

J Clin Orthod

June 2024

Department of Prosthodontics, Geriatric Dentistry and Craniomandibular Disorders/Section of CMD, Campus Benjamin Franklin; Center for Dental and Craniofacial Sciences (CC3), Charité-Universitätsmedizin Berlin; Freie Universität Berlin, Humboldt-Universität zu Berlin; Berlin Institute of Health, Berlin, Germany.

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Chronic inflammation poses challenges to effective cancer treatment. Although anti-inflammatory therapies have shown short-term benefits, their long-term implications may be unfavorable because they fail to initiate the necessary inflammatory responses. Recent research underscores the promise of specialized pro-resolving mediators, which play a role in modulating the cancer microenvironment by promoting the resolution of initiated inflammatory processes and restoring tissue hemostasis.

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Essential genes for survival and biofilm growth.

mSystems

September 2024

Department of Cell and Molecular Biology, University of Rhode Island, Kingston, Rhode Island, USA.

Article Synopsis
  • The text discusses a Gram-negative bacterium that is commonly found in the human mouth, serving as both a beneficial commensal microbe and a potential opportunistic pathogen outside the oral cavity.
  • Researchers used advanced sequencing techniques to identify essential and conditionally essential genes involved in biofilm formation of this bacterium under different oxygen conditions, finding significant core genes related to metabolism.
  • The study aims to enhance understanding of the bacterium's biology and its interactions within the oral environment, providing a foundation for future research on its role in oral health and disease.
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Inhibition of SARS-CoV-2 infection by and the oral microbiome.

Microbiol Spectr

October 2024

Department of Oral Science and Translational Research, Health Professions Division, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA.

Article Synopsis
  • The COVID-19 pandemic continues despite widespread vaccination, emphasizing the need for new therapeutic and preventive strategies, particularly through exploring the oral microbiome's role in SARS-CoV-2 infection.
  • Research revealed that certain compounds from oral bacteria, such as phosphoglycerol dihydroceramide (PGDHC) and gingipains, can inhibit SARS-CoV-2 infection, while other unidentified factors may interfere with the virus's ability to fuse with host cells.
  • Variability in oral microbial communities among individuals means some saliva cultures can inhibit while others may enhance infection, highlighting the complex relationship between oral bacteria and COVID-19, and suggesting potential avenues for new treatment options.
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Intermittent fasting exerts a profound beneficial influence on a spectrum of diseases through various mechanisms including regulation of immune responses, elimination of senescent- and pathogenic cells and improvement of stem cell-based tissue regeneration in a disease- and tissue-dependent manner. Our previous study demonstrated that alternate-day fasting (ADF) led to alleviation of xerostomia and sialadenitis in non-obese diabetic (NOD) mice, a well-defined model of Sjögren's syndrome (SS). This present study delved into the previously unexplored impacts of ADF in this disease setting and revealed that ADF increases the proportion of salivary gland stem cells (SGSCs), defined as the EpCAM cell population among the lineage marker negative submandibular gland (SMG) cells.

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Targeted elimination of alleviates periodontitis.

J Oral Microbiol

August 2024

Immunology and Infectious Diseases, The ADA Forsyth Institute, Cambridge, MA, USA.

Background: , a pathobiont in periodontal disease, contributes to alveolar bone destruction. We assessed the efficacy of a new targeted antimicrobial, FP-100, in eradicating from the oral microbial community and and evaluated its effectiveness in reducing bone loss in a mouse periodontitis model.

Methods: A multispecies bacterial community was cultured and treated with two concentrations of FP-100 over two days.

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Characterization of oral biomarkers during early healing at augmented dental implant sites.

J Periodontal Res

August 2024

Division of Periodontology, Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, Massachusetts, USA.

Article Synopsis
  • The study looked at how certain proteins (biomarkers) that help with healing show up in the mouth after dental surgery using connective tissue grafts (CTG).
  • It involved 28 people who had single dental implants with soft tissue problems, and they were treated in two different ways (CAF and TUN) while checking their healing over 3 months.
  • Important findings showed that some healing proteins (VEGF and TIMP-2) reacted differently depending on the treatment method, and higher levels of these proteins at 7 days were linked to better recovery later on.
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The unique epibiotic-parasitic relationship between type strain TM7x, a member of the newly identified Candidate Phyla Radiation, now referred to as and its basibiont, strain XH001 (formerly , require more powerful genetic tools for deeper understanding of the genetic underpinnings that mediate their obligate relationship. Previous studies have mainly characterized the genomic landscape of XH001 during or post TM7x infection through comparative genomic or transcriptomic analyses followed by phenotypic analysis. Comprehensive genetic dissection of the pair is currently cumbersome due to the lack of robust genetic tools in TM7x.

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Objectives: This study developed a sol-gel tricalcium silicate/graphene oxide (TCS-GO) composite and examined its physicochemical properties, antimicrobial activity, and osteo/odontogenic effect on dental pulp stem cells.

Methods: Tricalcium silicate was synthesized and combined with graphene oxide at three different concentrations, namely 0.02%, 0.

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