Isolation of PCSK9-specific nanobodies from synthetic libraries using a combined protein selection strategy.

Nanobodies (Nbs) hold great potential to replace conventional antibodies in various biomedical applications. However, conventional methods for their discovery can be time-consuming and expensive. We have developed a reliable protein selection strategy that combines magnetic activated cell sorting (MACS)-based screening of yeast surface display (YSD) libraries and functional ligand-binding identification by Tat-based recognition of associating proteins (FLI-TRAP) to isolate antigen-specific Nbs from synthetic libraries.

Pilot-scale phosphorus recovery from mobile toilet wastewater in Bangkok, Thailand.

The objective of this study is to enhance the capacity of struvite-phosphate forming reactor utilized in the production of phosphorus fertilizer from wastewater collected from mobile toilets, characterized by phosphorus (P) concentrations of 5.0 ± 1.1 g/l.

Resilience-building in palliative care professionals: scoping review.

Unlabelled: Resilience-building in palliative care professionals: scoping review BACKGROUND: Burnout, demoralisation and compassion fatigue are common among palliative care professionals. Practising palliative care necessitates a quality of resilience in order to ensure constant and optimal patient care. However, there is no universal approach to prevent burnout or raise resilience among palliative care professionals.

Molecular dynamics simulation revealing the interactions between toxic aldehydes and starch: A case study of malondialdehyde, 4-hydroxy-2-hexenal, and 4-hydroxy-2-nonenal in starch-based food during frying.

Toxic compounds in fried food products attract increasing attention, but studies regarding the levels of common toxins, such as malondialdehyde (MDA), 4-hydroxy-2-hexenal (4-HHE), and 4-hydroxy-2-nonenal (4-HNE), in fried starch-based food (FSBF) are scarce. Herein, we investigated the formation and distributions of these aldehydes in FSBF, and the frying oil and FSBF retained higher levels of low-molecular-weight aldehydes. The highest aldehyde concentrations were observed in French fries.

A comprehensive review of the impact of anthocyanins from purple/black Rice on starch and protein digestibility, gut microbiota modulation, and their applications in food products.

This review explores the impact of anthocyanins derived from purple and black rice on starch and protein digestibility, gut microbiota modulation, and their applications in food production. Anthocyanins are shown to reduce starch digestibility by forming complexes with starch, thereby inhibiting key digestive enzymes. Additionally, they can influence protein digestion by inducing structural changes that enhance resistance to digestive processes.

The tidal trap - Seasonal transport of floating macrodebris in the bi-directional Chao Phraya River network on the Gulf of Thailand.

Models estimate up to 3 million metric tons of river plastic waste flowing into the world's ocean every year. All ocean-bound rivers endure tidal impact to some degree, but there is a lack of data on the resulting marine emission effects. To address this gap we analyzed the trajectories of grapefruit-sized floating GPS drifters (n = 63) in the Chao Phraya estuary in Bangkok, Thailand, in the three seasons of 2022-2023.

Comparison of Five Pathological Tumor Regression Grading Systems for Rectal Cancer Following Chemoradiation: Correlation Coefficient and Intra-Rater Reliability.

Objective: To determine the correlation among five different types of tumor regression grading (TRG) systems. Test-retest reliability analyses were conducted at two time points to assess the internal validity and consistency of these five TRG systems.

Methods: A test-retest study was performed in 34 pathologically confirmed rectal adenocarcinoma specimens.

The feasibility of using photovoice as a loneliness intervention with older Myanmar migrants.

Loneliness has detrimental physical and mental health outcomes. To date, there are few studies on loneliness interventions in lower-resource settings. Based on participatory action research methods that are theoretically informed by the social relationship expectations framework, we developed a loneliness intervention called amanane using the photovoice method with older Myanmar migrants in northern Thailand.

Measurement of multidifferential cross sections for dijet production in proton-proton collisions at .

A measurement of the dijet production cross section is reported based on proton-proton collision data collected in 2016 at by the CMS experiment at the CERN LHC, corresponding to an integrated luminosity of up to 36.3 . Jets are reconstructed with the anti- algorithm for distance parameters of and 0.

Short- and Long-Term Outcomes of Patients Who Underwent the Arterial Switch Operation at Siriraj Hospital-Thailand's Largest National Tertiary Referral Center.

Objective: The aim of this study was to assess the short- and long-term outcomes of patients who underwent the arterial switch operation (ASO) at Siriraj Hospital in Thailand, and to identify postoperative complications and factors that significantly affect patient survival.

Materials And Methods: We retrospectively studied all patients with dextro-transposition of the great arteries and anatomic variants who underwent the ASO from January 1995 to December 2020. Twenty-year overall survival and 15-year freedom from reoperation/reintervention were estimated using the Kaplan-Meier method.

Effect of a modified methyl methacrylate-p-styrene sulfonic acid copolymer-based gel desensitizer on dentin permeability and tubule occlusion in human dentin .

Background/purpose: Tubular occlusion is an effective method to treat dentin hypersensitivity. This study aimed to determine the effect of a modified methyl methacrylate-p-styrene sulfonic acid copolymer-based gel desensitizer on dentin permeability and tubule occlusion in extracted human premolars.

Materials And Methods: Hydraulic conductance (HC) measurement (n = 50) and scanning electron microscopy (SEM; n = 64) were performed.

New insights into freshwater ascomycetes: discovery of novel species in diverse aquatic habitats.

During investigations of freshwater fungi in Hunan and Yunnan provinces, China, sp. nov. (Nectriaceae), sp.

Clinical Efficacy Between Intravenous Paracetamol and Intravenous Fentanyl for Propofol Deep Sedation in Colonoscopy: A Randomized Controlled Trial.

Introduction: Sedation practices for colonoscopy indeed vary widely around the globe. Due to a lack of data on intravenous paracetamol, we aimed to investigate the clinical efficacy of intravenous paracetamol compared to intravenous fentanyl under propofol deep sedation for colonoscopy.

Methods: A total of 225 patients who underwent colonoscopy at Siriraj Hospital were randomly assigned to two groups.

Transient receptor potential channels in dental inflammation and pain perception: A comprehensive review.

Transient Receptor Potential (TRP) channels are a family of ion channels that play pivotal roles in various physiological processes, including sensory transduction, temperature regulation, and inflammation. In the context of dentistry, recent research has highlighted the involvement of TRP channels in mediating sensory responses and inflammation in dental tissues and temporo-mandibular joint (TMJ) structure. TRP channels have emerged as major contributors in the development of inflammatory conditions and pain affecting the oral cavity and related structures, such as periodontitis, dental erosion cause hypersensitivity, pulpitis, and TMJ disorders.

Metallic Nanostructures: An Updated Review on Synthesis, Stability, Safety, and Applications with Tremendous Multifunctional Opportunities.

Metallic nanostructures play a vital role in technological advancement, providing exceptional performance and improved adaptability in comparison to their bulk equivalents. Conventional synthesis techniques frequently depend on dangerous reducing agents to transform metal ions into Nanoparticles (NPs), which presents considerable environmental and health issues. In contrast, the approach of green synthesis, which emphasizes the use of non-toxic reagents, has garnered significant interest as a sustainable method for the fabrication of Metallic Nanoparticles (MNPs).

A descriptive analysis of nurses' self-reported mental health symptoms during the COVID-19 pandemic: An international study.

Aim: To describe the self-reported mental health of nurses from 35 countries who worked during the COVID-19 pandemic.

Background: There is little occupationally specific data about nurses' mental health worldwide. Studies have documented the impact on nurses' mental health of the COVID-19 pandemic, but few have baseline referents.

The impact of sodium-glucose cotransporter-2 inhibitors on the incidence, therapy, and outcomes of fournier gangrene: insights from a systematic review of case reports.

Background: The clinical characteristics, therapy, and outcome of Fournier Gangrene (FG) in patients using sodium-glucose cotransporter-2 inhibitors (SGLT2i) were examined in this systematic review.

Methods: Without a publication year restriction, we searched PubMed, ScienceDirect, and Cochrane. Additionally, we manually searched bibliographies using the terms "Fournier's gangrene" and "SGLT2 inhibitors.

Pattern of antibiotic dispensing at pharmacies according to access, watch, reserve (AWaRe) classification: multicenter study after COVID-19 waves in different districts of Pakistan.

Objective: The dispensing of non-prescription antibiotics is a worldwide concern, and antibiotics should only be dispensed with a legitimate prescription.

Methodology: A cross-sectional study was conducted using the simulated client methodology, with data collected, recorded, and analyzed using SPSS.

Results: The simulated client visited 210 pharmacies, of which 70 dispensed antibiotics without a prescription.

Effectiveness of deliberate practices versus conventional lecture in trauma training for medical students.

Background: A mastery of life-threatening trauma procedures is important for medical students aiming to become proficient physicians. Thus, this study compares the effectiveness of deliberate practice with that of conventional lecture methods in teaching such students these essential skills.

Methods: A randomized controlled trial was conducted with 48 first- to third-year medical students at the Faculty of Medicine Vajira Hospital at Navamindradhiraj University (Thailand).

Prediction of difficult mask ventilation in Thai adult patients undergoing elective surgery using ultrasound of distance from skin to hyoid bone, and from skin to thyroid isthmus: a prospective cohort observational study.

Background: A previous study showed that airway ultrasound, specifically the distance from the skin to the hyoid bone (DSHB), may be correlated with a higher risk of difficult mask ventilation (DMV). However, the study was conducted in Italy and lacks data for the Asian and Thai populations. This study aimed to predict DMV using pre-operative ultrasonography to measure the DSHB and from the skin to the thyroid isthmus (DSTI) in Thai patients undergoing elective surgery under general anesthesia.

Factors predicting secondary hypertension in young adults with hypertension: a retrospective study.

Background: Hypertension in young adults is often due to secondary causes, and investigating these can be resource-intensive. This study aimed to identify clinical and biochemical markers that could suggest secondary hypertension in individuals under 40 years.

Materials And Methods: A 6-year retrospective observational cohort study included 207 young adults with hypertension who were assessed for secondary causes such as hyperthyroidism, primary aldosteronism, Cushing's syndrome, pheochromocytoma, and renovascular disease.

Efficacy, Safety, and Cost-effectiveness of Zinc Oxide Nanoparticles in Whitfield's Spirit Solution for Treating Superficial Fungal Foot Infections: A Randomized Controlled Trial.

Introduction: A novel antifungal formulation combining zinc oxide nanoparticles and Whitfield's spirit solution (ZnO-WFs) was developed to enhance the treatment of superficial fungal foot infections.

Methods: This 8-week, randomized, double-blinded controlled trial compared the efficacy, safety, and cost-effectiveness of ZnO-WFs with those of Whitfield's spirit solution (WFs) alone and a zinc oxide nanoparticle solution (ZnOs). Seventy of the 84 enrolled patients completed the trial.

Effect of anthocyanin rich black sugarcane on milk production and antioxidant capacity in lactating dairy cows.

This study aimed to explore the effect of anthocyanin-rich black sugarcane on milk production, plasma antioxidant capacity, and the storage period DPPH scavenging capacity of milk in lactating dairy cows. Sixteen lactating dairy cows were stratified and randomly assigned into two balanced dietary groups, namely Anthocyanin-rich black sugarcane (AS), and Napier grass (NG). The AS group demonstrated a significant decrease (p < 0.