Different fates between extracellular and intracellular antimicrobial resistome in full-scale activated sludge and membrane bioreactor processes.

Treated effluent of wastewater treatment plants (WWTPs) are major sources of extracellular antimicrobial resistance genes (eARGs) into aquatic environments. This study aimed to clarify the fate and origins of eARGs from influent to treated effluent at a full-scale WWTP. The compositions of eARG and intracellular ARG (iARG) were acquired via shotgun metagenomic sequencing in influent wastewater, activated sludge, and treated effluent of the target WWTP, where identical wastewater was treated by conventional activated sludge (CAS) and membrane bioreactor (MBR) processes.

Prevalence of FGFR2b Protein Overexpression in Advanced Gastric Cancers During Prescreening for the Phase III FORTITUDE-101 Trial.

Purpose: Fibroblast growth factor receptor 2 isoform IIIb (FGFR2b) protein overexpression is an emerging biomarker in gastric cancer and gastroesophageal junction cancer (GC). We assessed FGFR2b protein overexpression prevalence in nearly 3,800 tumor samples as part of the prescreening process for a global phase III study in patients with newly diagnosed advanced or metastatic GC.

Methods: As of June 28, 2024, 3,782 tumor samples from prescreened patients from 37 countries for the phase III FORTITUDE-101 trial (ClinicalTrials.

Knowledge, attitudes, and practices (KAP) of animal health staff and farmers towards brucellosis control in sheep and goat in China: A cross-sectional study.

Background: In China, brucellosis has resurfaced recently with a discernible spatial distribution, particularly affecting dairy herds and small ruminant populations. However, limited dissemination of knowledge, attitudes, and practices (KAP) for brucellosis control exists among farmers and animal health staff. This study aimed to assess the KAP of brucellosis control and prevention in animal health staff and farmers, with the goal of educating the public regarding the application of efficient brucellosis control and prevention strategies.

Performance of five dynamic models in predicting tuberculosis incidence in three prisons in Thailand.

This study examined the ability of the following five dynamic models for predicting pulmonary tuberculosis (PTB) incidence in a prison setting: the Wells-Riley equation, two Rudnick & Milton-proposed models based on air changes per hour and liters per second per person, the Issarow et al. model, and the applied susceptible-exposed-infected-recovered (SEIR) tuberculosis (TB) transmission model. This 1-year prospective cohort study employed 985 cells from three Thai prisons (one prison with 652 cells as the in-sample, and two prisons with 333 cells as the out-of-sample).

Potential Zoonotic Infections Transmitted by Free-Ranging Macaques in Human-Monkey Conflict Areas in Thailand.

Introduction: Nonhuman primates (NHPs) can transmit zoonotic diseases to humans because of their close genetic relationship, facilitating the cross-species transmission of certain pathogens. In Thailand, Macaca is the most common NHP genus and their inhabits area are in close proximity of human, particularly in urban and suburban areas, where frequent interactions with humans increase the risk of pathogen transmission. The risk is influenced by factors such as the type of pathogen, the mode of transmission (e.

α-Glucosidase inhibitory activities of polyphenols from Mesua ferrea L. leaves.

Mesua ferrea L. is used in Ayurvedic and Thai medicine for treating various diseases, including diabetes. This study aimed to isolate and identify the bioactive constituents from M.

Overcoming low status or maintaining high status? A multinational examination of the association between socioeconomic status and honour.

We examined the relationship between socioeconomic status (SES) and endorsement of honour. We studied the SES-honour link in 5 studies (N = 13,635) with participants recruited in different world regions (the Mediterranean and MENA, East Asian, South-East Asian, and Anglo-Western regions) using measures that tap into various different facets of honour. Findings from these studies revealed that individuals who subjectively perceived themselves as belonging to a higher (vs.

Diurnal Regulation of SOS Pathway and Sodium Excretion Underlying Salinity Tolerance of Vigna marina.

Vigna marina (Barm.) Merr. is adapted to tropical marine beaches and has an outstanding tolerance to salt stress.

Polychlorinated biphenyls induced toxicities upon cell lines and stem cells: a review.

Polychlorinated biphenyls (PCBs) are persistent organic pollutants emitted during e-waste activities. Upon release into the environment, PCBs can pose harmful effects to the humans and environment. The present review focused on the effects of PCBs on cell proliferation, apoptosis, functional and developmental toxicity and potential possible molecular mechanisms upon cells and stem cells.

Variations in land surface temperature increase in South-East Asian Cities.

Climate change and global warming are terms used to describe the variation in the Earth's mean temperature as a result of human activities contributing to the formation of urban heat islands (UHI). One method for determining the temperature of a region is the land surface temperature (LST). The study of LSTs is important and closely related to climate change, as is the provision of convenient living and working conditions in cities, which support economic growth.

Updated Nematocyst Types in Tentacle of Venomous Box Jellyfish, and (Cnidaria, Cubozoa) in Thai Waters.

The multiple-tentacle box jellyfish, (Sucharitakul, 2017) and (Horst, 1907), are venomous species found in Thai waters. They are responsible for numerous envenomations through their stinging organelles, nematocysts. These specialized microscopic structures discharge venom, yet detailed knowledge of their types and morphology in these species remains limited.

A Study of Combined Onabotulinumtoxin A and Hyaluronic Acid Filler for the Treatment of Enlarged Facial Pores.

Introduction: Enlarged facial pores are a common cosmetic concern caused by excessive sebum production, visible hair shafts, and a reduction in skin elasticity, leading to a decrease in skin quality and overall appearance. Various treatment modalities have been explored to address this issue. This study focuses on the efficacy and safety of combining Onabotulinumtoxin A (OnaBoNT-A) and hyaluronic acid filler (HA filler) to target enlarged facial pores in Asians.

Snake Venom Pharmacokinetics and Acute Toxic Outcomes Following Envenoming: Experimental and Clinical Correlations.

An understanding of snake venom pharmacokinetics is essential for determining clinical outcomes of envenoming and developing therapeutic approaches to the treatment of envenoming, especially regarding the timing and optimal dosage of antivenom administration. (Eastern Russell's viper) envenoming causes systemic coagulopathy and severe hemorrhage including acute kidney injury. These toxic outcomes can be diminished by the administration of high quantities of Russell's viper antivenom.

Host-Induced Gene Silencing of the -Methyl Transferase Gene Enhanced Maize Aflatoxin Resistance.

Maize is one of the major crops that are susceptible to infection and subsequent aflatoxin contamination, which poses a serious health threat to humans and domestic animals. Here, an RNA interference (RNAi) approach called Host-Induced Gene Silencing (HIGS) was employed to suppress the -methyl transferase gene (, also called ), a key gene involved in aflatoxin biosynthesis. An RNAi vector carrying part of the gene was introduced into the B104 maize line.

Black Soldier Fly () Larvae as a Protein Substitute in Adverse Food Reactions for Canine Dermatitis: Preliminary Results Among Patients.

Can black soldier fly (BSF) larvae be a protein substitute for managing adverse food reactions (AFRs) in dogs, specifically those with dermatitis? We evaluated BSF larvae's safety, tolerance, and effectiveness as a novel protein source in dogs with AFRs. Sixteen dogs, including eight healthy controls and eight diagnosed with AFRs, were fed a diet containing BSF larvae for four weeks. Skin conditions, including dermatological lesions and Pruritus Visual Analog Scale (PVAS) scores, and gastrointestinal signs, including relevant clinical signs, stool consistency, and hematological and biochemical parameters, were monitored.

Newcastle Disease Virus Displaying an Ectodomain of Middle East Respiratory Syndrome Coronavirus Spike Protein Elicited Robust Humoral and Cellular Immunity in Mice.

Background: Middle East Respiratory Syndrome Coronavirus (MERS-CoV) causes severe respiratory illness in humans and currently lacks an approved vaccine. The Newcastle disease virus (NDV) vector is a well-established, safe, and effective platform for vaccine development. With recent advancements in stabilizing coronavirus spike proteins to enhance their antigenicity, this study aimed to determine whether modifications to the MERS-CoV spike protein could improve its presentation on NDV particles, allowing the resulting virus to be used as an inactivated vaccine.

Analysis of Upper Facial Weakness in Central Facial Palsy Following Acute Ischemic Stroke.

Background: Central facial palsy (CFP), resulting from upper motor neuron lesions in the corticofacial pathway, is traditionally characterized by the sparing of the upper facial muscles. However, reports of upper facial weakness in CFP due to acute ischemic stroke have challenged this long-held assumption. This study aimed to determine the prevalence of upper facial weakness in CFP and identify its associated clinical factors.

Assessing the Feasibility of Using a Multiplex Serological Assay to Conduct Serosurveillance for Malaria Exposure in Deployed Military Personnel.

Reproducibly assessing malaria exposure is critical for force health protection for military service members deployed to malaria-endemic regions as well as for civilians making public health decisions and evaluating malaria eradication efforts. However, malaria disease surveillance is challenged by under-reporting, natural immunity, and chemoprophylaxis, which can mask malaria exposure and lead to an underestimation of malaria prevalence. In this study, we determined the feasibility of using a serosurveillance-based approach to measure Anopheles vector exposure, Plasmodium sporozoite exposure, and blood-stage parasitemia using a multiplex serological panel.

Development of Prevascularized Synthetic Block Graft for Maxillofacial Reconstruction.

Cranio-maxillofacial bone reconstruction, especially for large defects, remains challenging. Synthetic biomimetic materials are emerging as alternatives to autogenous grafts. Tissue engineering aims to create natural tissue-mimicking materials, with calcium phosphate-based scaffolds showing promise for bone regeneration applications.

Molecular Phylogeny and Morphology Reveal Four New Species of (Bolbitiaceae, Agaricales) from the Qinghai-Xizang Plateau, China.

The Qinghai-Xizang Plateau, known for its high altitude, geological history of plate collision, crustal uplift, and special ecology factors, provides an ideal environment for studying fungal biodiversity in extreme environmental conditions. Some species within the , containing secondary metabolites such as psilocybin, phallotoxins, and amatoxins, have potential medicinal value for treating psychiatric disorders and for use in drug development. This study investigates (Bolbitiaceae, Agaricales) on the Plateau, based on specimens collected over the past decade, using morphological and molecular phylogenetic analyses.

Development of BCC7051 as a Robust Cell Factory Towards the Transcriptional Regulation of Protease-Encoding Genes for Industrial Applications.

Enzyme-mediated protein degradation is a major concern in industrial fungal strain improvement, making low-proteolytic strains preferable for enhanced protein production. Here, we improved food-grade BCC7051 by manipulating the transcriptional regulation of protease-encoding genes. Genome mining of the transcription factor and computational analysis confirmed its deduced amino acid sequence sharing evolutionary conservation across and spp.

Combined Input Deep Learning Pipeline for Embryo Selection for In Vitro Fertilization Using Light Microscopic Images and Additional Features.

The current process of embryo selection in in vitro fertilization is based on morphological criteria; embryos are manually evaluated by embryologists under subjective assessment. In this study, a deep learning-based pipeline was developed to classify the viability of embryos using combined inputs, including microscopic images of embryos and additional features, such as patient age and developed pseudo-features, including a continuous interpretation of Istanbul grading scores by predicting the embryo stage, inner cell mass, and trophectoderm. For viability prediction, convolution-based transferred learning models were employed, multiple pretrained models were compared, and image preprocessing techniques and hyperparameter optimization via Optuna were utilized.

Application of Generative Artificial Intelligence Models for Accurate Prescription Label Identification and Information Retrieval for the Elderly in Northern East of Thailand.

This study introduces a novel AI-driven approach to support elderly patients in Thailand with medication management, focusing on accurate drug label interpretation. Two model architectures were explored: a Two-Stage Optical Character Recognition (OCR) and Large Language Model (LLM) pipeline combining EasyOCR with Qwen2-72b-instruct and a Uni-Stage Visual Question Answering (VQA) model using Qwen2-72b-VL. Both models operated in a zero-shot capacity, utilizing Retrieval-Augmented Generation (RAG) with DrugBank references to ensure contextual relevance and accuracy.

Arthroscopic foveal reattachment of triangular fibrocartilage complex: Inside-out technique at specific ulnocarpal anatomical landmarks.

This study evaluates the outcomes of arthroscopic triangular fibrocartilage complex (TFCC) foveal reattachment using an inside-out suture passing through the TFCC at specific ulnocarpal anatomical landmarks to grasp the radioulnar ligaments. Thirty-eight patients with a mean age of 36 years (range 19-54), diagnosed with TFCC injury with distal radioulnar joint (DRUJ) instability, underwent arthroscopic inside-out TFCC foveal reattachment using designated suture sites. At a mean follow-up of 32 months (range 26-44), pain score, range of motion, grip strength, Disabilities of the Arm, Shoulder and Hand (DASH) score, and Modified Mayo Wrist Score all showed significant improvement after surgery.