H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts.

Post-translational modifications of histone H3 on lysine 9, specifically acetylation (H3K9ac) and tri-methylation (H3K9me3), play a critical role in regulating chromatin accessibility. However, the role of these modifications in lineage segregation in the mammalian blastocyst remains poorly understood. We demonstrate that di- and tri-methylation marks, H3K9me2 and H3K9me3, decrease during cavitation and expansion of the rabbit blastocyst.

Insights into mungbean defense response to Cercospora leaf spot based on transcriptome analysis.

Several mungbean (Vigna radiata (L.) Wilczek) cultivars are susceptible to Cercospora leaf spot (CLS) caused by Cercospora canescens Ellis & Martin, and it is necessary to explore resistance sources and understand resistance mechanisms. However, the CLS resistance mechanisms have not yet been explored.

Phytosome-Encapsulated 6-Gingerol- and 6-Shogaol-Enriched Extracts from Roscoe Protect Against Oxidative Stress-Induced Neurotoxicity.

The rising prevalence of neurodegenerative disorders underscores the urgent need for effective interventions to prevent neuronal cell death. This study evaluates the neuroprotective potential of phytosome-encapsulated 6-gingerol- and 6-shogaol-enriched extracts from Roscoe (6GS), bioactive compounds renowned for their antioxidant and anti-inflammatory properties. The novel phytosome encapsulation technology employed enhances the bioavailability and stability of these compounds, offering superior therapeutic potential compared to conventional formulations.

Analysis of printing temperature effect on texture modification: Potential of soy protein isolate-based bigel for swallowing-friendly food.

This work prepared the soy protein isolate (SPI)-beeswax-based bigel loaded with β-carotene, and the effect of printing temperature (PT) on texture regulation was investigated. During printing, increasing PT weakened the rheological properties and printability of ink. However, the mechanical strength and deformation resistance at non-linear regions of products were strengthened after printing.

A new type III effector from Bradyrhizobium sp. DOA9 encoding a putative SUMO-protease blocks nodulation in Arachis hypogaea L.

Effector proteins secreted via the type III secretion system (T3SS) of nitrogen-fixing rhizobia are key determinants of symbiotic compatibility in legumes. Previous report revealed that the T3SS of Bradyrhizobium sp. DOA9 plays negative effects on Arachis hypogaea symbiosis.

Optimizing extraction conditions for isolation of antioxidant compounds from black rice bran using ultrasonic-assisted extraction and response surface methodology.

Black rice bran (BRB), a valuable byproduct from the rice milling process, possesses numerous pharmacological activities, including antioxidant potential, but information regarding highly efficient extraction methods is scarce. To enhance the extraction efficiency, ultrasonic-assisted extraction coupled with Box-Behnken design (BBD) was used in this study to maximize the total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), and antioxidant capacity of BRB extract. The BBD results showed that 57% ethanol at 50°C and pH 3.

Partially substituting roughage with traditional Chinese herbal medicine residues in the diet of goats improved feed quality, growth performance, hematology, and rumen microbial profiles.

This study aimed to reveal the effect of traditional Chinese herbal medicine residues (TCHMR) on growth performance, hematology, ruminal microbiota, and economic benefits of Guizhou black male goats through the fermented total mixed ration (FTMR) diet technique. A total of 22 Guizhou black male goats with an initial weight of 21.77 ± 0.

Extract from endophytic isolates stimulates seed germination of the host and protocorm development of non-host orchids.

We isolated endophytic strains from the healthy roots, stems, and leaves of to investigate their plant growth-promoting activities in vitro. Subsequently, Indole acetic acid (IAA) was quantified and the gene (responsible for IAA synthesis in fungi) was amplified and sequenced. Finally, a germination assay was performed with seeds of and a plant growth assay with protocorms of to test their plant growth-promoting activities.

Oral administration of C37 inhibits colonization in chicks.

As the global population continues to grow, so too does the demand for poultry meat. However, the concurrent increase in the prevalence of drug-resistant bacteria has stimulated interest in the search for alternatives to antibiotics in poultry and livestock agriculture. One potential strategy is the use of probiotics.

Research advancements on the diversity and host interaction of gut microbiota in chickens.

The maintenance of host health and immune function is heavily dependent on the gut microbiota. However, the precise contribution of individual microbial taxa to regulating the overall functionality of the gut microbiome remains inadequately investigated. Chickens are commonly used as models for studying poultry gut microbiota, with high-throughput 16S rRNA sequencing has emerged as a valuable tool for assessing both its composition and functionality.

Modeling the effect of daytime duration on the biosynthesis of terpenoid precursors.

Terpenoids are valued chemicals in the pharmaceutical, biotechnological, cosmetic, and biomedical industries. Biosynthesis of these chemicals relies on polymerization of Isopentenyl di-phosphate (IPP) and/or dimethylallyl diphosphate (DMAPP) monomers, which plants synthesize using a cytosolic mevalonic acid (MVA) pathway and a plastidic methyleritritol-4-phosphate (MEP) pathway. Circadian regulation affects MVA and MEP pathway activity at three levels: substrate availability, gene expression of pathway enzymes, and utilization of IPP and DMAPP for synthesizing complex terpenoids.

The Field Automatic Insect Recognition-Device-A Non-Lethal Semi-Automatic Malaise Trap for Insect Biodiversity Monitoring: Proof of Concept.

Field monitoring plays a crucial role in understanding insect dynamics within ecosystems. It facilitates pest distribution assessment, control measure evaluation, and prediction of pest outbreaks. Additionally, it provides important information on bioindicators with which the state of biodiversity and ecological integrity in specific habitats and ecosystems can be accurately assessed.

Production of functional bovine IL-22 in a mammalian episomal expression system.

Interleukin 22 is a member of the interleukin-10 superfamily of cytokines. This protein has a dual role as an inflammatory and anti-inflammatory molecule dependent on the context. IL-22 is produced mainly by immune cells and seems to have non-hematopoietic cells as its target.

Proteomic analysis revealed the deterioration of surimi gelling capability to fish stress during transportation.

Live silver carp with various transportation durations (3 h, 6 h, 12 h and 24 h) was processed into surimi. Proteomics and other technical methods were applied to investigate the changes in protein composition and structure aiming to explore the mechanism through which transportation stress affects gelling capability of surimi. As the transportation time was prolonged from 3 to 24 h, the gel strength of surimi gel decreased by 28.

Future of reproductive biotechnologies in water buffalo in Southeast Asian countries.

The future of reproductive biotechnologies in water buffalo in Southeast Asian countries holds significant promise for enhancing genetic quality and productivity. Fixed-time artificial insemination remains the commonly used technology, with advances in assisted reproductive technologies (ART) such as in vitro embryo production (IVEP), embryo transfer (ET), and the use of sex-sorted sperm increasingly adopted to improve breeding efficiency. These technologies overcome traditional breeding limitations, such as low reproductive rates, genetic diversity constraints, and the production of sex-predetermined offspring.

Temporal appearance of embryonic compartments and their sexual dimorphism in chicken.

1. Building on established understandings regarding hormonal and metabolic-driven processes of avian embryo's growth and development, this paper proposes a novel method, focusing on sex-related dimorphism. Such processes involve crucial activities, .

Enzymatic hydrolysis of duck blood protein produces stable bioactive peptides: Pilot-scale production, identification, and stability during gastrointestinal and plasma digestion.

This study addresses the valorization of duck blood, an underutilized protein-rich by-product from the poultry industry, into bioactive protein hydrolysates with antioxidant and ACE inhibitory properties. Raw and heat-treated duck blood were compared as substrates for enzymatic hydrolysis using Neutrase and Papain. Gel electrophoresis revealed that heat treatment reduced fibrinogen content, while FTIR analysis showed that heat treatment modified the protein structure, increasing β-sheet content from 21.

Conditioned medium of human umbilical cord-mesenchymal stem cells cultivated with human cord blood serum enhances stem cell stemness and secretome profiles.

The proteins secreted by human umbilical cord mesenchymal stem cells (hUC-MSCs) may enhance tissue regeneration and wound healing. Traditional hUC-MSC cultures may not be enough since they undergo recurrent cellular senescence during large-scale production. This decreases the therapeutic ability of hUC-MSCs by altering genes and proteins that control stemness, proliferation, and protein release.

Transcriptome analysis of jejunal mucosal tissue in breeder hens exposed to acute heat stress.

Heat stress (HS) severely compromises intestinal barrier function in poultry, resulting in significant production losses. This study aimed to explore the molecular response of the small intestine to acute HS in breeder hens. Fifty 28-week-old breeder hens were raised individually in a cage and randomly assigned to control and heat-treated groups (25 hens each).

Effects of replacement soybean meal with fermented cassava pulp by Saccharomyces cerevisiae in DIETS on Rumen fermentation in growing goats.

The aim of this study was to determine the effect of replacing soybean meal (SBM) with fermented cassava pulp by Saccharomyces cerevisiae (FCSC) in the diet of growing goats. Growing goats were randomly assigned to five dietary treatments according to replicated 5 × 5 Latin square design. Dietary treatments were five levels of replacement SBM to FCSC at 0, 25, 50, 75, and 100% of crude protein in concentrates.

Bacillus velezensis S141 improves the root growth of soybean under drought conditions.

Bacillus velezensis S141 helps soybean establish specific symbiosis with strains of Bradyrhizobium diazoefficiens to form larger nodules and improve nitrogen fixation efficiency. In this study, we found that the dry weight of soybean roots increased significantly in the presence of S141 alone under drought conditions. Hence, S141 improved the root growth of soybean under limited water supply conditions.