GZ17-6.02 interacts with carboplatin and etoposide to kill neuroblastoma cells.

The biology of GZ17-6.02 alone and more so in combination with either of the standard-of-care agents etoposide or carboplatin killed MYCN overexpressing neuroblastoma (NB) cells is unknown. The methods involved in this study are in-cell immunoblotting, trypan blue exclusion, plasmid and siRNA transfection, assessment of autophagy using a plasmid expressing LC3-GFP-RFP.

Pridopidine in Amyotrophic Lateral Sclerosis: The HEALEY ALS Platform Trial.

Importance: Amyotrophic lateral sclerosis (ALS) is a fatal disease. The sigma-1 (σ1) receptor emerged as a target for intervention.

Objective: To determine the effects of pridopidine, a σ1-receptor agonist, in ALS.

Verdiperstat in Amyotrophic Lateral Sclerosis: Results From the Randomized HEALEY ALS Platform Trial.

Importance: Myeloperoxidase is one of the most abundant peroxidase enzymes in activated myeloid cells. Myeloperoxidase inhibitors may have a clinical benefit in amyotrophic lateral sclerosis (ALS) by slowing neurodegeneration via reduced neuroinflammation and oxidative stress.

Objective: To determine the safety, tolerability, and efficacy of verdiperstat, a selective myeloperoxidase inhibitor, in ALS.

A prospective multicenter validation of RETREAT for post-transplantation hepatocellular carcinoma recurrence prediction.

Background: The RETREAT score is a simple risk stratification tool for post-liver transplantation (LT) hepatocellular carcinoma (HCC) recurrence that has been validated in retrospective cohort studies. A prospective, multicenter study is needed to further demonstrate accuracy especially given evolving clinical demographics and HCC transplant practice.

Aim: To validate and compare the RETREAT score to other post-LT HCC recurrence risk scores in a contemporary, prospective cohort of patients.

Acute pulmonary embolism treatment in lung transplant recipients: mechanical thrombectomy and catheter directed thrombolysis.

Purpose: Acute pulmonary embolism (PE) presents a significant challenge in lung transplant recipients (LTR), even with prophylactic anticoagulation. Due to the heightened risk of complications in this population, the optimal treatment approach for acute PE remains uncertain. This retrospective case series aims to elucidate the outcomes of percutaneous mechanical thrombectomy with the Inari device (MT) and catheter-directed thrombolysis (CDT) in managing acute PE in lung transplant patients.

MicroRNAs in lung cancer: their role in tumor progression, biomarkers, diagnostic, prognostic, and therapeutic relevance.

MicroRNAs (miRNAs) are a class of small non-coding RNAs which are associated with post-transcriptional regulation of gene expression. Dysfunction or aberrant expression of miRNAs is predominant in various malignancies including lung cancer. Lung cancer is one of the commonest causes of cancer-related death worldwide, with a five-year survival of only 10-20%.

Accounting for unmet needs resulting from cancer-related cognitive impairment.

Purpose: Cancer-related cognitive impairment (CRCI) causes a wide range of unmet needs for cancer survivors. It is unknown which clinical, demographic, cognitive, and psychological factors underpin and account for these various unmet needs. This study aimed to (a) identify factors associated with CRCI-related unmet needs, and (b) establish the most pertinent factors that account for CRCI-related unmet needs.

Neuromodulation of Fear and Anxiety Circuits.

Anxiety is a complex and heterogeneous condition consisting of multiple component processes and neural underpinnings. Recent neural accounts for anxiety have expanded beyond the canonical anxiety and fear circuitry centered on the amygdala to a distributed network. A burgeoning form of clinical intervention with remarkable potential to directly engage the neural substrates of anxiety is non-invasive brain stimulation (NIBS), which is especially notable for the ability to modulate macro- and meso-scopic circuits and networks.

Serum Metabolomic Markers of Dietary Potassium and Risk of Chronic Kidney Disease.

Background: Discovering metabolomic markers of dietary potassium may help improve dietary assessment of potassium and trace the impact of dietary potassium on chronic kidney disease (CKD) development.

Methods: We included adults from the Atherosclerosis Risk in Communities (ARIC) study without CKD at visit 1 (N = 3,812). Cross-sectional associations between dietary potassium and serum metabolites were assessed using multivariable linear regression models.

Tumor-specific surface marker-independent targeting of tumors through nanotechnology and bioorthogonal glycochemistry.

Biological targeting is crucial for effective cancer treatment with reduced toxicity but is limited by the availability of tumor surface markers. To overcome this, we developed a nanoparticle-based, Tumor-specific suRfACE maRker-independent (TRACER) targeting approach. Utilizing the unique biodistribution properties of nanoparticles, we encapsulated Ac4ManNAz to selectively label tumors with azide reactive groups.

Vascular architecture-on-chip: engineering complex blood vessels for reproducing physiological and heterogeneous hemodynamics and endothelial function.

Human circulation exhibits significant diversity and heterogeneity of blood vessel shapes. The complex architecture of these vessels may be physiological or pathological resulting in unique hemodynamics and endothelial cell phenotypes that may determine the regulation and alteration of cell signaling pathways and vascular function. While human microphysiological systems of blood vessels (vessel-chips) have mimicked several aspects of vascular pathophysiology, engineering of these tools is still limited to the fabrication of homogeneous tubular structures, especially when living endothelial cell culture is also included.

Bowel Disorder Incidence and Rectal Spacer Use in Patients With Prostate Cancer Undergoing Radiotherapy.

Importance: The polyethylene glycol-based hydrogel spacer (PHS) system temporarily separates the rectum from the prostate in patients undergoing radiotherapy (RT) for prostate cancer (PCa).

Objective: To compare incidence of bowel disorders and related procedures in patients receiving RT with and without PHS.

Design, Setting, And Participants: This retrospective cohort study used 4 datasets: Medicare 5% Standard Analytic Files, Medicare 100% Standard Analytic Files, Merative MarketScan Commercial Database, and Premier Healthcare Database.

Screening for Food Insecurity: US Preventive Services Task Force Recommendation Statement.

Importance: According to survey data, 12.8% of households experienced food insecurity in 2022, with 7.7% of households experiencing low food security and 5.

Long-Term Functional Outcomes of Free Flaps Versus Locoregional Flaps in Soft Tissue Reconstruction for Oral Cavity Cancer: A Systematic Review.

Background: The choice between free flaps and locoregional flaps for soft tissue reconstruction in oral cavity cancer patients is critical for determining long-term functional and oncological outcomes. This systematic review evaluates the efficacy of these reconstructive techniques, focusing on survival, recurrence, quality of life (QoL), and functional parameters such as speech, swallowing, and the need for gastrostomy or tracheostomy.

Methods: A systematic review adhering to PRISMA guidelines was conducted using PubMed, Scopus, Cochrane, and EBSCO databases.

Effects of social environment and energy efficiency on preferred swim speed in a marine generalist fish, pile perch Phanerodon vacca.

Energy efficiency is a key component of movement strategy for many species. In fish, optimal swimming speed (Uopt) is the speed at which the mass-specific energetic cost to move a given distance is minimised. Additional factors may, however, influence an individual's preferred swimming speed (Upref).

Dry Needling in United States Doctor of Physical Therapy Programs: Safety Assessment and Adverse Event Reporting.

Introduction: Despite the growing clinical use of dry needling (DN) among physical therapists in the United States and its gradual adoption into entry-level Doctor of Physical Therapy (DPT) programs, limited research exists on DN safety within educational settings. This study aims to report the type and incidence of adverse events (AEs) associated with DN instruction in DPT programs.

Review Of Literature: To date, there has been no reporting on DN-related AEs during DPT education, making the relative risk of DN education unclear.

GSK3 coordinately regulates mitochondrial activity and nucleotide metabolism in quiescent oocytes.

As cells transition between periods of growth and quiescence, their metabolic demands change. During this transition, cells must coordinate changes in mitochondrial function with the induction of biosynthetic processes. Mitochondrial metabolism and nucleotide biosynthesis are key rate-limiting factors in regulating early growth.

Implementing the 2023 Entry-Level Physical Therapist Curriculum Recommendations for Integumentary/Wound Management in Doctor of Physical Therapy Education.

Background And Purpose: Entry-level physical therapist education programs must adequately prepare graduates to effectively assess, manage, and/or refer patients with foundational integumentary/wound management (IWM) concerns in any population or setting. Unfortunately, the literature reports wide variations in the number of IWM contact hours in entry-level health professions education, as well as discrepancies in specific content covered. Standardization in curricular content through consensus-based curriculum guidelines decreases treatment variability and promotes safe, evidence-based practice across a profession.

ILAE neuroimaging task force highlight: The utility of multimodal neuroimaging in diagnostic and presurgical workup of drug-resistant focal epilepsy.

The ILAE Neuroimaging Task Force publishes educational case reports that highlight basic aspects of neuroimaging in epilepsy, consistent with ILAE's educational mission. In patients with drug-resistant focal epilepsy who are candidates for surgical intervention, the identification of structural abnormalities is a strong predictor of favorable postoperative seizure outcomes. When conventional imaging is insufficient, the integration of multimodal neuroimaging data with structural, metabolic, and functional imaging modalities is often helpful.

Social Determinants of Health and Access to Allogeneic Hematopoietic Cell Transplantation for Acute Myeloid Leukemia.

Whether allogeneic hematopoietic cell transplant (allo-HCT) to treat acute myeloid leukemia (AML) is equitably accessible regardless of social determinants of health (SDOH) remains unknown. We examined associations of SDOH with access to allo-HCT and other outcomes. Patients presenting for treatment (n=692) at 13 AML treatment centers were prospectively recruited to a registered clinical trial (#NCT01929408).

Emulating Target Trials to Study Perioperative and Critical Care Interventions with Observational Data: Promise and Limitations.

Estimating effects of interventions is a central task in perioperative and critical care outcomes research. While randomized trials remain the accepted standard for causal inference, trial data are not always available to inform clinical decisions, and some questions cannot be answered feasibly or efficiently with trials. In these settings, studies using observational healthcare data may be used to inform practice.

Perfluorooctane Sulfonate (PFOS) and Related Compounds Induce Nuclear Receptor 4A1 (NR4A1)-Dependent Carcinogenesis.

Polyfluoroalkyl substances (PFAS) are widely used industrial compounds that have been identified as contaminants in almost every component of the global ecosystem, and in human studies, higher levels of PFAS have been correlated with increased incidence of multiple diseases. Based on the results of human and laboratory animal studies, we hypothesize that the orphan nuclear receptor 4A1 (NR4A1) may be a critical target for some PFAS such as the legacy linear polyfluorooctanesulfonate (PFOS) and other sulfonates. We show that PFOS and related compounds bound the ligand binding domain (LBD) of NR4A1 and induced the growth of several cancer cell lines and enhanced tumor growth in an athymic nude mouse model.

Single-Item Screening for Depression in Adults (50+) with History of Traumatic Brain Injury.

Background: Single-item depression screening has shown utility in young adult samples with limited work focused on individuals 50 and older. We examined single-item depression screening in adults 50 and older with a history of TBI one year post-injury.

Method: This project involved secondary analysis of deidentified TRACK-TBI data.