The Role of Advanced Technologies Supplemented with Traditional Methods in Pharmacovigilance Sciences.

Background: The immediate automatic systemic monitoring and reporting of adverse drug reactions, improving the efficacy is the utmost need of the medical informatics community. The venturing of advanced digital technologies into the health sector has opened new avenues for rapid monitoring. In recent years, data shared through social media, mobile apps, and other social websites has increased manifolds requiring data mining techniques.

In vitro and in situ abrogation of biofilm formation in E. coli by vitamin C through ROS generation, disruption of quorum sensing and exopolysaccharide production.

Emergence of antimicrobial drug-resistance amongst food-borne pathogens has led to severe deficit of available therapeutics and requires novel interventions. This study determined the activity of vitamin C (VitC), a natural antioxidant as powerful antibacterial agent against multidrug-resistant (MDR), biofilm-forming E. coli.

New zinc complexes derived from "self-adaptable" acyclic diiminodipyrromethanes as potent catalysts for the reduction of curing temperature of bisphenol-A/F benzoxazines.

The simple modification of the Schiff-base ligands often brings significant changes in the coordination properties of the metal-complexes, providing newer prospects for their unexplored applications. In this context, the present work utilized the "self-adaptable" acyclic diiminodipyrromethane Schiff's bases (2a and 2b) for the synthesis of their Zn-based complexes and explored their potential in the ring-opening polymerization of benzoxazines. The two zinc complexes of composition [Zn{(Ph)(CH)C(2,6-PrCH-N[double bond, length as m-dash]CH-CHN)(2,6-PrCH-N[double bond, length as m-dash]CH-CHNH)}] (3) and [ZnCl{(Ph)(CH)C(PhC-NH[double bond, length as m-dash]CH-CHN)}] (4) were synthesized in good yields, and the structures were confirmed by single crystal X-ray diffraction (XRD).

Application of IgY antibodies against staphylococcal protein A (SpA) of Staphylococcus aureus for detection and prophylactic functions.

In the present study, immunoglobulin Y (IgY) antibodies were raised in hens against the surface staphylococcal protein A (SpA) of Staphylococcus aureus. Anti-SpA IgY were tested in vitro for diagnostic applications, bacteriostatic, and biofilm inhibition effects. A specific and sensitive immunocapture PCR (IPCR) was developed to detect S.

Techniques to Control Microbial Contaminants in Nonsterile Microalgae Cultivation.

The aim of this mini-review with own results was an identification of techniques to suppress the growth of microbial contaminants under photo- and mixotrophic conditions. Techniques identified are the modification of environmental conditions, such as pH, oxygen, and nutrient concentrations, as well as the application of pulsed electric field, ultrasonication, and surfactants. In phototrophic cultivations, the mentioned techniques result in a decrease of number of predatory cells, but not in a complete removal.

Binuclear Ni(II) complexes containing ONS donor Schiff base ligands: Preparation, spectral characterization, X-ray crystallography and biological exploration.

Four binuclear Ni(II) complexes [[Ni(H-DEAsal-tsc)(μ-dppm)]·2Cl (1), [Ni(DEAsal-mtsc)(μ-dppm)] (2), [Ni(DEAsal-etsc)(μ-dppm)] (3) and [Ni(DEAsal-ptsc)(μ-dppm)] (4)] were synthesized from the ligands namely 4(N,N)-diethylaminosalicylaldehyde-4(N)-thiosemicarbazone [H-DEAsal-tsc] HL/4(N,N)-diethylaminosalicylaldehyde-4(N)-methyl thiosemicarbazone [H-DEAsal-mtsc] HL/4(N,N)-diethylaminosalicylaldehyde-4(N)-ethyl thiosemicarbazone [H-DEAsal-etsc] HL/4(N,N)diethylaminosalicylaldehyde-4(N)-phenyl thiosemicarbazone [H-DEAsal-ptsc] HL and 1,1'-bis(diphenylphosphino)methane (dppm) and characterized by a number of spectro analytical techniques. The molecular structure of complexes [Ni(H-DEAsal-tsc)(μ-dppm)]·2Cl (1) and [Ni(DEAsal-ptsc)(μ-dppm)] (4) have been confirmed by single crystal X-ray diffraction studies. The analysis indicated that in complex 1, the ligand [H-DEAsal-tsc] coordinated as monobasic tridentate donor through phenolic oxygen, azomethine nitrogen and thione sulfur atoms.

Functional characterization of a broad and potent neutralizing monoclonal antibody directed against outer membrane protein (OMP) of Salmonella typhimurium.

In the present study, we have generated a murine monoclonal antibody (mAb) named Sal-06 by using the crude outer membrane protein preparation of Salmonella enteric subsp. enterica serovar Typhimurium ATCC 14028 strain as antigen. Sal-06mAb belonging to IgG1 isotype demonstrated broad cross-reactivity to standard and isolated strains of genus Salmonella and others such as Escherichia coli, Klebsiella pneumonia, and Proteus mirabilis.

Green pest management practices for sustainable buildings: Critical review.

Green pest management is an environmental-friendly pest management that helps to control pests and reduces harmful use of pesticides. Pests like woodworms, longhorn beetles, fruit flies, spiders, termites and paper mites in the buildings degrade the carpets, leather, wood, cloth, food, utensils and so on. They can spread diseases, damage properties and contaminate food in our home.

Huntington's chorea, a neurological disorder of all ages - Bioinformatics approach for its precise diagnosis.

Background: The disease that obstructs social movements of people is Huntington's disease (HD). Hence, the expedition of its molecular aspects and bioinformatics tools relating to precise confirmation of the disease is warranted. Due to social stigma, the care and attention to safeguard these patients had increased at community level.

Aqueous extract from bark protects cells from oxidative stress caused by electron beam radiation: approach.

In an endeavor to find the novel natural radioprotector to secure normal cells surrounding cancerous cell during radiation exposure, () aqueous stem bark extract was evaluated for radioprotective activity using , and models. extract exhibited concentration dependent protective effect on electron beam radiation (EBR) induced damage to pBR322 DNA; the highest protection was achieved at 150 μg concentrations. Similarly, extract (400 mg/kg) administrated to mice prior to irradiation protected DNA from the radiation damage, which was confirmed by inhibiting comet parameters.

Exploring the Potential of Capsaicin Against Cancer Metastasis Based on TGF-β Signaling Modulation Through Module-based Network Pharmacology Approachx.

Background: Capsaicin is an active alkaloid /principal component of red pepper responsible for the pungency of chili pepper. Capsaicin by changing the intracellular redox homeostasis regulate a variety of signaling pathways ultimately producing a divergent cellular outcome. Several reports showed the potential of capsaicin against cancer metastasis, however unexplored molecular mechanism is still an active part of the research.

A novel tandem repeat cloning technique for creation of multiple short peptide repeats to differentiate closely related antigens.

Antibody cross-reactivity is a problem often associated with closely related antigens. This study was aimed to develop a method enabling differentiation of closely related toxins based on antigen designing strategy. The method involves identification of disparate amino acids (AA) confined to target antigen in comparison with two or more closely related antigens, their assembly into a DNA oligomer and further cloning as six tandem repeats (TR) using restriction and ligation strategy into a desired vector and finally generation of antigen specific antibodies.

Natural radioprotectors and their impact on cancer drug discovery.

Cancer is a complex multifaceted illness that affects different patients in discrete ways. For a number of cancers the use of chemotherapy has become standard practice. Chemotherapy is a use of cytostatic drugs to cure cancer.

Bioethanol production from agarophyte red seaweed, , using a novel sample preparation method for analysing bioethanol content by gas chromatography.

In this study, is investigated for ethanol production. A combination of factors including different temperatures, acid concentration and incubation time was evaluated to determine the suitable saccharification conditions. The combination of 2.

Expression, purification, and characterization of pyruvate kinase from : A key allosteric regulatory enzyme.

Background: The current research aims to isolate pyruvate kinase (Pyk) gene from Mycobacterium tuberculosis and expression of the gene (Rv1617) to obtain a purified enzyme. The enzyme activity and secondary structural features were assessed through biochemical assays and circular dichroism (CD) spectroscopy, respectively.

Methods: The Pyk-encoding gene from the complete genome of M.

wound healing activity of Roxb aqueous leaf extract.

This study investigated the wound healing properties of the aqueous extract of Roxb leaves in rats. The study also provides information on the purification, qualitative, and quantitative analysis of phytochemical components present in Roxb. Wound contraction and period of epithelialization was determined and topical application of Roxb aqueous leaf extract showed better healing than orally treated and control groups.

Guava Leaf Extract Diminishes Hyperglycemia and Oxidative Stress, Prevents -Cell Death, Inhibits Inflammation, and Regulates NF-kB Signaling Pathway in STZ Induced Diabetic Rats.

Traditional Chinese medication has been utilized by Chinese medical practitioners to treat the varied symptoms of diabetes mellitus (DM). Notably, guava leaf has been used to treat diabetes in Asia. Our present study has been designed to analyze the action of guava leaf extract (GLE) at the molecular level in treating DM.

Industrial potential of carotenoid pigments from microalgae: Current trends and future prospects.

Microalgae are rich source of various bioactive molecules such as carotenoids, lipids, fatty acids, hydrocarbons, proteins, carbohydrates, amino acids, etc. and in recent Years carotenoids from algae gained commercial recognition in the global market for food and cosmeceutical applications. However, the production of carotenoids from algae is not yet fully cost effective to compete with synthetic ones.