Antibiotic-induced ROS-mediated Fur allosterism contributes to resistance by inhibiting activation of and .

The increasing antibiotic resistance of primarily driven by genetic mutations poses a significant clinical challenge. Although previous research has suggested that antibiotics could induce genetic mutations in , the molecular mechanisms regulating the antibiotic induction remain unclear. In this study, we applied various techniques (e.

IGF1R stimulates autophagy, enhances viability, and promotes insulin secretion in pancreatic β cells in gestational diabetes mellitus by upregulating ATG7.

Gestational diabetes mellitus (GDM) is a prevalent metabolic disturbance in pregnancy. This article investigated the correlations between serum IGF1R and ATG7 with insulin resistance (IR) in GDM patients. Firstly, 100 GDM patients and 100 healthy pregnant women were selected as study subjects.

MonkeyTrail: A scalable video-based method for tracking macaque movement trajectory in daily living cages.

Behavioral analysis of macaques provides important experimental evidence in the field of neuroscience. In recent years, video-based automatic animal behavior analysis has received widespread attention. However, methods capable of extracting and analyzing daily movement trajectories of macaques in their daily living cages remain underdeveloped, with previous approaches usually requiring specific environments to reduce interference from occlusion or environmental change.

Mapping blood traits to structural organization of the brain in rhesus monkeys.

Hematological and biochemical blood traits have been linked to brain structural characteristics in humans. However, the relationship between these two domains has not been systematically explored in nonhuman primates, which are crucial animal models for understanding the mechanisms of brain function and developing therapeutics for various disorders. Here we investigated the associations between hematological/biochemical parameters and the brain's gray matter volume and white matter integrity derived from T1-weighted and diffusion magnetic resonance imaging in 36 healthy macaques.

Extending indigenous knowledge to unveil the evolutionary journey of food preferences and socio-cultural phenomena.

Food acceptance is substantially influenced by eating behavior and society norms in relation to preferences, lifestyles, and socio-cultural practices. Food innovation communities need to comprehend the impact of changing trends in eating behavior as a driving force. Meanwhile, many studies indicate that indigenous knowledge plays an important role in uncovering various social phenomena and interpreting local holistic views, cultural ecology, and behavior.

Bioballs carrying a syntrophic Rhodococcus and Mycolicibacterium consortium for simultaneous sorption and biodegradation of fuel oil in contaminated freshwater.

Nonpathogenic effective bacterial hydrocarbon degraders, Rhodococcus ruber S103, Mycolicibacterium parafortuitum J101 and Mycolicibacterium austroafricanum Y502, were isolated from mixed polycyclic aromatic hydrocarbon (PAH)-enriched river sediments. They possessed broad substrate specificities toward various PAHs and aliphatic compounds as sole carbon sources. These strains exhibited promising characteristics, including biosurfactant production, high cell hydrophobicity, biofilm formation and no antagonistic interactions, and contained genes encoding hydrocarbon-degrading enzymes.

Assessment of the economic recycling potential of a glycolysis treatment of rigid polyurethane foam waste: A case study from Thailand.

This research assessed the economic feasibility of recovering polyol from rigid polyurethane foam (RPUF) waste, collected from dismantled, discarded refrigerators, by a single-phase glycolysis recycling process using domestic crude glycerol (a byproduct of biodiesel production) as a transesterification agent. The study, conducted in Thailand, took into account benefits from the added value of the recovered product, the investment and related operating costs of the process, demand (the need for the recovered product) and supply (the amount of RPUF waste). The results showed that the production of recovered polyol from RPUF waste is economically feasible, with a net present value of 1,113,018 USD, an internal rate of return (IRR) of 22.

Rapid and sensitive PCR-dipstick DNA chromatography for multiplex analysis of the oral microbiota.

A complex of species has been associated with dental caries under the ecological hypothesis. This study aimed to develop a rapid, sensitive PCR-dipstick DNA chromatography assay that could be read by eye for multiplex and semiquantitative analysis of plaque bacteria. Parallel oligonucleotides were immobilized on a dipstick strip for multiplex analysis of target DNA sequences of the caries-associated bacteria, Streptococcus mutans, Streptococcus sobrinus, Scardovia wiggsiae, Actinomyces species, and Veillonella parvula.

Tag/hybridization-based sensitive detection of polymerase chain reaction products.

The polymerase chain reaction (PCR) is an important technology to amplify a single copy or a few copies of DNA segment in genomic DNAs, visualizing the segment as DNA fragment. Thus, PCR is frequently used in various examinations such as detection of bacteria and fungi in the food industry. Here, we report a simple and sensitive method for detection of PCR products using single-strand tag sequence and hybridization of the tag sequence to the complementary tag sequence immobilized on solid material (STH).