Diphthamide synthesis is linked to the eEF2-client chaperone machinery.

The diphthamide modification of eukaryotic translation elongation factor (eEF2) is important for accurate protein synthesis. While the enzymes for diphthamide synthesis are known, coordination of eEF2 synthesis with the diphthamide modification to maintain only modified eEF2 is unknown. Physical and genetic interactions extracted from BioGRID show a connection between diphthamide synthesis enzymes and chaperones in yeast.

Multinational retrospective analysis of bridging therapy prior to chimeric antigen receptor t cells for relapsed/refractory acute lymphoblastic leukemia in children and young adults.

Anti-CD19 chimeric antigen receptor T cells (CAR) are a well-established treatment option for children and young adults suffering from relapsed/refractory B-lineage acute lymphoblastic leukemia. Bridging therapy is used to control disease prior to start of lymphodepletion before CAR infusion and thereby improve efficacy of CAR therapy. However, the effect of different bridging strategies on outcome, side effects and response to CAR therapy is still poorly understood.

Biochemical failure-free survival of F-rhPSMA-7 and F-flotufolastat PET-guided salvage radiotherapy for patients with recurrent prostate cancer.

Prostate-specific membrane antigen (PSMA)-targeted positron emission tomography (PET) has improved localization of prostate cancer (PC) lesions in biochemical recurrence (BCR) for salvage radiotherapy (SRT). We conducted a retrospective review of patients undergoing F-rhPSMA-7 or F-flotufolastat (F-rhPSMA-7.3)-PET-guided SRT compared with conventional-SRT (C-SRT) without PET.

How knee muscles and ground reaction forces shape knee buckling and ankle push-off in neuromuscular simulations of human walking.

Ankle push-off is important for efficient, human-like walking, and many prosthetic devices mimic push-off using motors or elastic elements. The knee is extended throughout the stance phase and begins to buckle just before push-off, with timing being crucial. However, the exact mechanisms behind this buckling are still unclear.

German evidence- and consensus-based guideline on the management of penile urethritis.

Urethritis is a common condition predominantly caused by sexually transmitted pathogens such as Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium. It is not possible to differentiate with certainty between pathogens on the basis of clinical characteristics alone. However, empirical antibiotic therapy is often initiated in clinical practice.

Redistribution of soil water by mature trees towards dry surface soils and uptake by seedlings in a temperate forest.

Hydraulic redistribution is considered a crucial dryland mechanism that may be important in temperate environments facing increased soil drying-wetting cycles. We investigated redistribution of soil water from deeper, moist to surface, dry soils in a mature mixed European beech forest and whether redistributed water was used by neighbouring native seedlings. In two experiments, we tracked hydraulic redistribution via (1) H labeling and (2) O natural abundance.

Analysis of radiation oncology integration within general practitioners' daily patient care: a cross-sectional survey in Germany.

Purpose: General practitioners (GPs) play a crucial role in providing interdisciplinary care for radiation oncology patients. This study aims to understand the specific needs and challenges faced by general practitioners in Germany when treating oncology patients.

Methods: A comprehensive web-based questionnaire with 24 items was disseminated to GPs in Germany via email using survio.

Biomechanics of flail chest injuries: tidal volume and respiratory work changes in multiple segmental rib fractures.

Background: Flail chest (FC) injuries are segmental osseous injuries of the thorax that typically result from high-energy blunt trauma and regularly occur in multiple trauma (MT) patients. FC injuries are associated with paradoxical chest wall movements and, thus, have a high risk of respiratory insufficiency or even death. An increasing number of studies recommend an early surgical stabilization of FC injuries, but a definite trigger that would indicate surgery has, thus far, not been identified.

Contribution of hypoxia-inducible factor 1alpha to pathogenesis of sarcomeric hypertrophic cardiomyopathy.

Hypertrophic cardiomyopathy (HCM) caused by autosomal-dominant mutations in genes coding for structural sarcomeric proteins, is the most common inherited heart disease. HCM is associated with myocardial hypertrophy, fibrosis and ventricular dysfunction. Hypoxia-inducible transcription factor-1α (Hif-1α) is the central master regulators of cellular hypoxia response and associated with HCM.

High-resolution X-ray phase-contrast tomography of human placenta with different wavefront markers.

Phase-contrast micro-tomography ([Formula: see text]CT) with synchrotron radiation can aid in the differentiation of subtle density variations in weakly absorbing soft tissue specimens. Modulation-based imaging (MBI) extracts phase information from the distortion of reference patterns, generated by periodic or randomly structured wavefront markers (e.g.

Cell wall polysaccharides from macauba pulp (Acrocomia aculeata L.): Fractionation and characterization of their chemical and rheological properties.

Macauba fruit pulp (Acrocomia aculeata) is an emerging oil source. After de-oiling, the macauba pulp meal (MPM) offers a dietary fiber content of 40-50 %, which mainly comprises cell wall polysaccharides (CWP). The present work aimed to assess the potential of MPM as an innovative source of sustainable food polysaccharides.

Catalytic effects of iron adatoms in poly(-phenylene) synthesis on rutile TiO(110).

-Armchair graphene nanoribbons (nAGNRs) are promising components for next-generation nanoelectronics due to their controllable band gap, which depends on their width and edge structure. Using non-metal surfaces for fabricating nAGNRs gives access to reliable information on their electronic properties. We investigated the influence of light and iron adatoms on the debromination of 4,4''-dibromo--terphenyl precursors affording poly(-phenylene) (PPP as the narrowest GNR) wires through the Ullmann coupling reaction on a rutile TiO(110) surface, which we studied by scanning tunneling microscopy and X-ray photoemission spectroscopy.

Non-random mating behaviour between diverging littoral and pelagic three-spined sticklebacks in an invasive population from Upper Lake Constance.

Adaptive divergence and increased genetic differentiation among populations can lead to reproductive isolation. In Lake Constance, Germany, a population of invasive three-spined stickleback () is currently diverging into littoral and pelagic ecotypes, which both nest in the littoral zone. We hypothesized that assortative mating behaviour contributes to reproductive isolation between these ecotypes and performed a behavioural experiment in which females could choose between two nest-guarding males.

Mind-mindedness in mothers of infants with excessive crying/sleeping/eating disorders.

Introduction: Excessive crying, sleeping, and eating disorders are among the most prevalent mental health diagnoses in the first 3 years of life and involve significant health service use. Parents of infants with excessive crying/sleeping/eating disorders report high levels of stress, since they feel incapable of soothing and/or nurturing their baby. Infants' distress can lead to a breakdown in parents' mentalizing abilities and, more specifically, parental mind-mindedness in the parent-child interaction.

Pervasive glacier retreats across Svalbard from 1985 to 2023.

A major uncertainty in predicting the behaviour of marine-terminating glaciers is ice dynamics driven by non-linear calving front retreat, which is poorly understood and modelled. Using 124919 calving front positions for 149 marine-terminating glaciers in Svalbard from 1985 to 2023, generated with deep learning, we identify pervasive calving front retreats for non-surging glaciers over the past 38 years. We observe widespread seasonal cycles in calving front position for over half of the glaciers.

Towards a comprehensive definition of the human fascial system.

The absence of a clear consensus on the definition and significance of fascia and the indiscriminate use of the term throughout the clinical and scientific literature has led to skepticism regarding its importance in the human body. To address this challenge, we propose that: (1) fasciae, and the fascial interstitia within them, constitute an anatomical system, defined as a layered body-wide multiscale network of connective tissue that allows tensional loading and shearing mobility along its interfaces; (2) the fascial system comprises four anatomical organs: the superficial fascia, musculoskeletal (deep) fascia, visceral fascia, and neural fascia; (3) these organs are further composed of anatomical structures, some of which are eponymous; (4) all these fascial organs and their structural components contain variable combinations and arrangements of the four classically defined tissues: epithelial, connective, muscle, and neural; (5) the overarching functions of the fascial system arise from the contrasting biomechanical properties of the two basic types of layers distributed throughout the system: one predominantly collagenous and relatively stiff, the other rich in hyaluronic acid and viscous, allowing for the free flow of fluid; (6) the topographical organization of these layers in different locations is related to local variations in function (e.g.

The spatial zonation of the murine placental vasculature is specified by epigenetic mechanisms.

The labyrinthian fetoplacental capillary network is vital for proper nourishment of the developing embryo. Dysfunction of the maternal-fetal circulation is a primary cause of placental insufficiency. Here, we show that the spatial zonation of the murine placental labyrinth vasculature is controlled by flow-regulated epigenetic mechanisms.

Merging motoneuron and postural synergies in prosthetic hand design for natural bionic interfacing.

Despite the advances in bionic reconstruction of missing limbs, the control of robotic limbs is still limited and, in most cases, not felt to be as natural by users. In this study, we introduce a control approach that combines robotic design based on postural synergies and neural decoding of synergistic behavior of spinal motoneurons. We developed a soft prosthetic hand with two degrees of actuation that realizes postures in a two-dimensional linear manifold generated by two postural synergies.

Bio- and Chemoinformatic Approaches for Metabolomics Data Analysis.

Metabolomics data analysis includes, next to the preprocessing, several additional repetitive tasks that can however be heavily dataset dependent or experiment setup specific due to the vast heterogeneity in instrumentation, protocols, or also compounds/samples that are being measured. To address this, various toolboxes and software packages in Python or R have been and are being developed providing researchers and analysts with bioinformatic/chemoinformatic tools to create their own workflows tailored toward their specific needs. This chapter presents tools and example workflows for common tasks focusing on the functionality provided by R packages developed as part of the RforMassSpectrometry initiative.

Clinical and magnetic resonance imaging outcome after proximal hamstring tendon repair at mean 3 years follow-up.

Purpose: The purpose of this study was to assess clinical and radiological outcome in patients after proximal hamstring tendon repair. We hypothesized that there is a significant correlation among subjective clinical outcome and interlimb asymmetries in muscle strength, fatty infiltration, and hamstring volume.

Methods: This retrospective monocentric case series included patients with surgical repair after proximal hamstring tendon rupture.

Combining Cre-LoxP and single-cell sequencing technologies: insights into the extracellular vesicle cargo transfer.

The recent study from the Pogge von Strandmann group published in , by Alashkar Alhamwe ., combined for the first time the Cre-LoxP recombination system with single-cell sequencing. The group monitored the tumor-derived extracellular vesicle (EV) uptake and the EV functions in the recipient non-malignant cells in a pancreatic ductal adenocarcinoma mouse model.