Point-of-care system for rapid real-time detection of SARS-CoV-2 virus based on commercially available Arduino platforms.

The COVID-19 pandemic emphasized the importance of rapid, portable, and on-site testing technologies necessary for resource-limited settings for effective testing and screening to reduce spreading of the infection. Realizing this, we developed a fluorescence-based point-of-care (fPOC) detection system with real-time reverse transcriptase loop-mediated isothermal amplification for rapid and quantitative detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus. The system is built based on the Arduino platform compatible with commercially available open-source hardware-software and off-the-shelf electronic components.

A Sensitive, Specific and Simple Loop Mediated Isothermal Amplification Method for Rapid Detection of spp. in Broiler Production.

Campylobacteriosis is one of the most common foodborne diseases worldwide. Two species - and in poultry and poultry products are considered to be the main source of human campylobacteriosis. Therefore, studying status in poultry flocks is needed to prevent transmission of disease and reduce human risk, health cost, and economic losses.

Classification of Multiple DNA Dyes Based on Inhibition Effects on Real-Time Loop-Mediated Isothermal Amplification (LAMP): Prospect for Point of Care Setting.

LAMP has received great interest and is widely utilized in life sciences for nucleic acid analysis. To monitor a real-time LAMP assay, a fluorescence DNA dye is an indispensable component and therefore the selection of a suitable dye for real-time LAMP is a need. To aid this selection, we investigated the inhibition effects of twenty-three DNA dyes on real-time LAMP.