8 results match your criteria: "Technical University Wildau[Affiliation]"

Qualitative and quantitative protease activity tests based on protein degradation in three-dimensional structures.

Bioelectrochemistry

December 2024

Biosystems Technology, Institute of Life Sciences and Biomedical Technologies, Technical University Wildau, Germany. Electronic address:

The pattern of the activity of proteases is related to distinct physiological states of living organisms. Often activity changes of a certain protease can be assigned to a specific disease. Hence, they are useful biomarkers and a simple and fast determination method of their activity could be a valuable tool for the efficient monitoring of numerous diseases.

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Quantitative determination of dopamine in the presence of interfering substances supported by machine learning tools.

Bioelectrochemistry

June 2024

Biosystems Technology, Institute of Life Sciences and Biomedical Technologies, Technical University Wildau, Hochschulring 1, 15745 Wildau, Germany. Electronic address:

In the field of neuroscience as well as in the clinical setting, the neurotransmitter dopamine (DA) is an analyte which is important for research as well as medical purposes. There are plenty of methods available to measure dopamine quantitatively, with voltammetric ones such as differential pulse voltammetry (DPV) being among the most convenient and simple ones. However, dopamine often occurs, either naturally or because of the requirements of involved enzymatic systems, alongside substances that can influence the signal it produces upon electrochemical conversion.

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Light-controlled imaging of biocatalytic reactions via scanning photoelectrochemical microscopy for multiplexed sensing.

Chem Commun (Camb)

May 2020

Biosystems Technology, Institute of Life Sciences and Biomedical Technologies, Technical University Wildau, Hochschulring 1, D-15745 Wildau, Germany.

A light-controlled multiplexing platform has been developed on the basis of a quantum dot-sensitized inverse opal TiO electrode with integrated biocatalytic reactions. Spatially resolved illumination enables multiplexed sensing and imaging of enzymatic oxidation reactions at relatively negative applied potentials.

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A precursor-approach in constructing 3D ITO electrodes for the improved performance of photosystem I-cyt c photobioelectrodes.

Nanoscale

August 2019

Biosystems Technology, Institute of Life Sciences and Biomedical Technologies, Technical University Wildau, 15745 Wildau, Hochschulring 1, Germany.

In recent years the use of photoelectrodes based on conductive metal oxides has become very popular in the field of photovoltaics. The application of 3D electrodes holds great promise since they can integrate large amounts of photoactive proteins. In this study photosystem I (PSI) from the thermophilic cyanobacterium Thermosynechococcus elongatus was immobilized on 3D ITO electrodes and electrically wired via the redox protein cytochrome c (cyt c).

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Integration of Enzymes in Polyaniline-Sensitized 3D Inverse Opal TiO Architectures for Light-Driven Biocatalysis and Light-to-Current Conversion.

ACS Appl Mater Interfaces

January 2018

Biosystems Technology, Institute for Applied Life Sciences, Technical University Wildau, Hochschulring 1, D-15745 Wildau, Germany.

Inspired by natural photosynthesis, coupling of artificial light-sensitive entities with biocatalysts in a biohybrid format can result in advanced photobioelectronic systems. Herein, we report on the integration of sulfonated polyanilines (PMSA1) and PQQ-dependent glucose dehydrogenase (PQQ-GDH) into inverse opal TiO (IO-TiO) electrodes. While PMSA1 introduces sensitivity for visible light into the biohybrid architecture and ensures the efficient wiring between the IO-TiO electrode and the biocatalytic entity, PQQ-GDH provides the catalytic activity for the glucose oxidation and therefore feeds the light-driven reaction with electrons for an enhanced light-to-current conversion.

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Connecting quantum dots with enzymes: mediator-based approaches for the light-directed read-out of glucose and fructose oxidation.

Nanoscale

February 2017

Biosystems Technology, Institute for Applied Life Sciences, Technical University Wildau, Hochschulring 1, D-15745 Wildau, Germany.

The combination of the biocatalytic features of enzymes with the unique physical properties of nanoparticles in a biohybrid system provides a promising approach for the development of advanced bioelectrocatalytic devices. This study describes the construction of photoelectrochemical signal chains based on CdSe/ZnS quantum dot (QD) modified gold electrodes as light switchable elements, and low molecular weight redox molecules for the combination with different biocatalysts. Photoelectrochemical and photoluminescence experiments verify that electron transfer can be achieved between the redox molecules hexacyanoferrate and ferrocene, and the QDs under illumination.

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Article Synopsis
  • A new photo-electrochemical sensor has been developed for detecting guanosine monophosphate (GMP) using a series of three enzyme reactions that ensure specificity and accurate measurement.
  • The sensor works by converting GMP to GDP through guanylate kinase, then using pyruvate kinase and lactate dehydrogenase for subsequent conversions that lead to the electrochemical detection of lactate.
  • The sensor is built using polyelectrolytes for immobilizing the enzymes and utilizes a CdS/ZnS quantum dot electrode that generates a photocurrent response when illuminated.
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This study reports on the oxygen sensitivity of quantum dot electrodes modified with CdSe/ZnS nanocrystals. The photocurrent behavior is analyzed for dependence on pH and applied potential by potentiostatic and potentiodynamic measurements. On the basis of the influence of the oxygen content in solution on the photocurrent generation, the enzymatic activity of glucose oxidase is evaluated in solution.

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