73 results match your criteria: "Technical Center for Animal[Affiliation]"

Short-term exposure to low doses of aflatoxin B1 aggravates nonalcoholic steatohepatitis by TLR4-mediated necroptosis.

Free Radic Biol Med

January 2025

Sanya Institute of Nanjing Agricultural University, Sanya, 572025, China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China; Institute of Animal Nutritional Health, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China. Electronic address:

Aflatoxin B1 (AFB1), a worldwide mycotoxin found in food and foodstuffs, is a potent hepatotoxin in humans and animals. Non-alcoholic fatty liver disease (NAFLD), a widespread disease, could progress from simple steatosis to non-alcoholic steatohepatitis (NASH), hepatic cirrhosis, and even hepatocellular carcinoma (HCC). To date, little is known concerning the relationship between AFB1 and the progression of NAFLD.

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The prevalence and impact of Getah virus (GETV) are significant concerns in China. GETV can infect a wide range of animals, including horses, pigs, sheep, cattle, birds, and humans, resulting in substantial losses in the livestock and agricultural industries. GETV infection can cause the development of ulcers and inflammation in the mouth and gums of horses, which result in pain and discomfort and lead to symptoms such as reduced appetite, drooling, and difficulty chewing.

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The viable but nonculturable (VBNC) state occurs when bacteria lose their ability to grow and multiply on conventional media when stressed by adverse environmental factors, but they remain active and can revive under certain conditions, posing a food safety risk. In this study, the VBNC state of Listeria monocytogenes was induced with different temperatures combined with low nutrient conditions; the VBNC state of L. monocytogenes was confirmed in conjunction with the housekeeping gene abcZ using a molecular biology assay (PMA-qPCR) to calculate the viable bacterial count; The resuscitation conditions for the VBNC state of L.

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Analysis of the Distribution Pattern of in China under Climate Change Based on the Biomod2 Model.

Biology (Basel)

July 2024

Key Laboratory of Surveillance and Management for Plant Quarantine Pests, Ministry of Agriculture and Rural Affairs, College of Plant Protection, China Agricultural University, Beijing 100193, China.

The changing global climate has significantly impacted the spread of plant pests. The cassava mealybug () is among the most dangerous quarantine pests affecting cassavas worldwide, causing substantial losses in agricultural production and food security across several regions. Although China is currently free of the cassava mealybug, its proximity to affected countries and extensive trade with these regions necessitate a detailed understanding of the pest's distribution pattern and dynamic ecological niche changes.

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A method for identifying specific peptide biomarkers of animal-milk-derived components in camel milk and its products was established using proteomics. Samples were prepared by defatting, protein extraction, and trypsin hydrolysis, and proteins and peptides were identified using ultra-high performance liquid chromatography-quadrupole/electrostatic orbitrap-high resolution mass spectrometry (UHPLC-Q/Exactive-HRMS) and Protein Pilot software. Twenty two peptide biomarkers from eight species (i.

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Molecular characterization of strains isolated from imported food in China from 14 countries/regions, 2003-2018.

Front Cell Infect Microbiol

January 2024

Technical Center for Animal, Plant and Food Inspection and Quarantine of Shanghai Customs, Shanghai, China.

() is associated with severe foodborne infections and ubiquitous in the nature. Identification of characteristics of transmission through trading of food products is essential for rapidly tracking sources and controlling dissemination of listeriosis. In this study, a total of 44 strains were isolated from food products originating from 14 countries/regions during 2003-2018 at the Shanghai port.

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Ultra-sensitive photoelectrochemical biosensor for determination of African swine fever virus based on surface plasmon resonance.

Anal Chim Acta

October 2023

Key Laboratory of Modern Agricultural Equipment and Technology (Jiangsu University), Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, PR China; School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address:

Sensitive and specific detection of African swine fever virus (ASFV) is crucial for agricultural production and economic development due to the mortality and infectivity. In this study, a bismuth induced enhanced photoelectrochemical (PEC) biosensor based on in-situ loop mediated isothermal amplification (LAMP) was constructed using deposited bismuth nanoparticles loaded bismuth oxycarbonate (Bi/(BiO)CO) as photoactive material, using primers designed according to LAMP as recognition elements, and using in-situ LAMP to achieve nucleic acid amplification of target genes. As the Bi induced surface plasmon resonance (SPR) effect, enhanced light captures and effective electron hole separation, it could effectively enhance the photoelectric activity, so the prepared Bi/(BiO)CO nanohybrid had higher photocurrent intensity and good stability.

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A systematic study was carried out on 638 wheat and paddy grains (including fresh and stored samples) collected in 2021 from Shanghai, China, to identify the major mycobiota and their toxigenic abilities. A total of 349 fungi, namely, 252 , 53 , and 44 , were characterized by morphological and molecular identification. and were more frequently isolated in paddy with species complex and section as the predominant species, respectively.

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Six species of from Tibet are described as new to science: , , , , , and A key to the species from Tibet of the genus is provided. The distribution of the genus in Tibet is also discussed.

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Development of a reverse-transcription droplet digital PCR method for quantitative detection of .

Heliyon

February 2023

Technical Center for Animal, Plant and Food Inspection and Quarantine of Shanghai Customs District, Shanghai 200135, China.

(CGMMV) is a re-emerging threat to the production of greenhouse cucumber and other Cucurbitaceae crops worldwide. This seed-borne virus can easily spread from a contaminated seed to seedlings and adjacent plants by mechanical contact between the foliage of diseased and healthy plants, causing extensive yield losses. An accurate method for detecting and quantifying this virus is urgently needed to ensure the safety of the global seed trade.

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A CRISPR activation screen identifies genes that enhance SARS-CoV-2 infection.

Protein Cell

January 2023

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai 200032, China.

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For environmental safety, it is important to establish a simple, rapid, and sensitive method for emerging pollutants. Here, a dispersive solid-phase extraction (d-SPE) method based on an iron-based metal-organic framework (Fe-MIL-88-NH) combined with high-performance liquid chromatography (HPLC) was developed for tetrabromobisphenol A (TBBPA) in water samples. Fe-MIL-88-NH was synthesized using a solvothermal method and completely characterized.

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First Report of Fusarium acuminatum Causing Dianthus chinensis root rot and foliage blight in China.

Plant Dis

November 2022

Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China, NANJING, jiangsu, China;

Article Synopsis
  • Dianthus chinensis, an ornamental plant, faced a disease outbreak in May 2020 in Nanjing, China, affecting over half of the tested one-year-old plants with symptoms like foliage blight and root discoloration.
  • The pathogen was isolated from infected samples through a series of laboratory procedures, leading to the growth of distinct hyphae and three representative isolates that were stored for further research.
  • Genetic sequencing of the isolates showed significant similarities to previously documented strains, allowing researchers to explore their phylogenetic relationships and enhance understanding of the disease's characteristics.
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Photinia × fraseri Dress was introduced to China at the end of the 20th century. It is a kind of colorful ornamental tree species with great ornamental value. From 2020 to 2021, a disease was found in Railway Gymnasium, Xuanwu district of Nanjing which approximately 40% P.

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Rapid detection of based on a new target gene .

Front Cell Infect Microbiol

September 2022

Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.

causes crown and root wilting in more than 5,000 plant species and represents a significant threat to the health of natural ecosystems and horticultural crops. The early and accurate detection of is a fundamental step in disease prevention and appropriate management. In this study, based on public genomic sequence data and bioinformatic analysis of several , , and species, we have identified a new target gene, Pcinn13739; this allowed us to establish a recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay for the detection of .

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Risk assessment and early warning of the presence of heavy metal pollution in strawberries.

Ecotoxicol Environ Saf

September 2022

School of life Science, Shanghai University, 99 Shangda Road, Shanghai 200444, PR China. Electronic address:

Heavy metal pollution is a major threat to agricultural produce and it can pose potential ecological risks which subsequently impacts on human health. Strawberries are an economically important produce of China. The intrinsic link of heavy metal pollution risk in the soil-strawberry ecosystem is of concern.

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Bluetongue (BT) causes an economic loss of $3 billion every year in the world. After two serious occurrences of BT (bluetongue virus [BTV] occurrence in 2006 and 2015), France has been controlling for decades, but it has not been eradicated. As the largest live cattle export market in the world, France is also one of the major exporters of breeding animals and genetic materials in the world.

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Unlabelled: Bovine A1-or A2-type β-caseins have attracted a growing interest due to their variation in beta-casomorphin-7 (BCM-7) formation, which may affect health. In the present work, identification and quantification of A1 and A2 types of β-casein proteins at the peptide level was achieved for the first time. An automated and online immobilized trypsin digestion system was employed for high throughput digesting of proteins into peptides.

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(), a seed-borne parasitic nematode, is the causal agent of rice white tip disease (RWTD), which may result in a drastic loss of rice yield. Seed treatments are currently considered to be the most effective means of preventing the spread of RWTD. Therefore, the rapid, highly specific, and accurate detection of from rice seeds is crucial for the surveillance, prevention, and control of RWTD.

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A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice.

Commun Biol

July 2022

National Center for the Molecular Characterization of Genetically Modified Organisms, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Efficient, accurate molecular characterization of genetically modified (GM) organisms is challenging, especially for those transgenic events transferred with genes/elements of recipient species. Herein, we decipher the comprehensive molecular characterization of one novel GM rice event G281 which was transferred with native promoters and an RNA interference (RNAi) expression cassette using paired-end whole genome sequencing (PE-WGS) and modified TranSeq approach. Our results show that transgenes integrate at rice chromosome 3 locus 16,439,674 included a 36 bp deletion of rice genomic DNA, and the whole integration contains two copies of the complete transfer DNA (T-DNA) in a head-to-head arrangement.

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Photinia × fraseri Dress is a hybrid species of Rosaceae and Photinia genus which is widely cultivated in China. During 2020 and 2021, approximately 80% of plants growing in Xuanwu district of Nanjing, China, exhibited disease symptoms including blight, necrosis, and dieback of crowns and roots. Symptomatic root tissues collected from 2-year-old plants were rinsed with water, cut into 2-mm tissues which were surface-sterilized in 70% ethanol for 60 s, and plated onto 10% V8 PARP agar and incubated in the dark at 26°C for 3 days.

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Article Synopsis
  • * The process involves extracting samples with acetonitrile, purifying them with a solid-phase extraction column, and using a chiral column for separation under specific conditions, detected by an ultraviolet detector.
  • * The method shows high reliability with low limits of quantification (0.2 mg/kg), excellent recovery rates (80.6-105%), and a good correlation for linearity, making it effective and environmentally friendly for testing food purity.
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Article Synopsis
  • - Photinia × fraseri Dress, an ornamental plant introduced to China in 1998, suffered a significant blight and root discoloration disease at Xuanwu Lake Park, affecting about 80% of the population between April 2021 and 2022.
  • - Research involved isolating the causative fungal pathogen, which produced typical Fusarium structures, including macroconidia and microconidia, confirming its identity as F. oxysporum through genetic analysis.
  • - Pathogenicity tests demonstrated that seedlings inoculated with the fungal isolates developed crown and root rot, while control plants remained unaffected, highlighting the pathogenic nature of the identified fungus.
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Abstract: Veterinary drugs, including antibiotics, antiparasitics, and growth promoters, are widely used in animal husbandry. Veterinary drug residues are key issues of food safety because they arouse public concern and can seriously endanger the health of consumers. To assess the risk of veterinary drug residues in pork sold in the People's Republic of China, the potential veterinary drug residue risks in imported and domestic pork were analyzed based on regulatory differences and veterinary drug residue safety incidents.

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