[Interaction of annexin VI with actin].

Actin polymerization was investigated using fluorescence probe N-(1-pyrenyl)iodoacetamide, which was bound covalently to reactive sulfhydryl group, Cys-373. Labeled actin in the bulk was 0.5 to 1% of total actin concentration.

[Catalytic characteristics of 5'-nucleotidase in the structure of cell membrane rafts in smooth muscles].

5'-nucleotidase (EN 3.1.3.

[Interferon-induced 2',5'-oligoadenylate system: key components and biological functions].

The current data about the 2',5'-oligoadenylate system is reviewed. Its role in interferon signaling and cell metabolism regulation is discussed. The interferon system is known to be characterized by a wide range of biological functions such as antiviral defense, control of cell growth and differentiation, oncogenic stability, apoptosis, immune activation, etc.

[Platelet membrane Ca(2+) and Na(+), K(+)-ATPase activity and aggregation in myelodysplastic syndrome].

Platelet aggregation was decreased under action of ADP and collagen in patients with myelodysplastic syndrome. The decrease in aggregation started from 3-rd minutes and decreased in 4 and 5 minutes after action of ADP. The study of Ca(2+)-ATPase and Na+, K(+)-ATPase membrane activities showed the decrease in Ca(2+)-ATPase and increase in Na+, K(+)-ATPase activity in the patients with myelodysplastic syndrome.

[Particulars on the regulation of Ca(2+) in the cell nucleus].

The data of literature on the structural and functional features of nuclear pore complexes, the role of receptor-activated Ca(2+)-channels and Ca(2+)-ATPase of nuclear membranes are presented. The possible ways of Ca(2+)-signal transmission into the nucleus and the role of nuclear Ca2+ in apoptosis are discussed.

Solid-phase reagent for molecular spectroscopic determination of heavy metal speciation in natural water.

A solid-phase reagent based on 1-(4-adamantyl-2-thiasolylazo)-2-naphthol adsorbed onto silica gel was prepared for Co(II) recovery and preconcentration prior to its sorption-spectroscopic detection. The immobilized reagent was applied to the determination of free cobalt ions in natural water. The solid-phase reagent and chemiluminescent method coupled with membrane filtration, gel-permeation and ion-exchange chromatography were applied to the study of the speciation of iron and cobalt in water from the Dnieper reservoirs and lakes of Kyiv City; their predominant forms were complexes of Fe(III) and Co(II) with dissolved organic matter and fulvic acids play a main role in their complexation.

[Investigation of the activity of the preparation cerbiden against Candida spp].

Antifungal activity of a new complex antibiotical preparation cerbiden obtained from medical plant Bidens cernua L. of the Asteraceae family, was investigated in vitro against the clinical and museum strains of Candida spp. High cerbiden activity against clinical and museum strains of Candida albicans, C.

[Effect of the substances "Ammivit" and "cerloplasmin" on lipid peroxidation products level and of antioxidant system indicators under irradiation].

It has been shown, that the total X-ray irradiation in the dozes of 0.5 and 1 Gy influences on the content of lipid peroxidation products and enzymatic activity of antioxidant system in rat spleen and thymus cells. The influence of preparations "AMMIVIT" and "Ceruloplasmin" on these processes is investigated also.

[Immunosuppressive components of extracellular lipopolysaccharide highly virulent strain Salmonella typhimurium 1468].

Immunosuppressive activity of culture liquid substrate (CFS) of highly virulent strain Salmonella typhimurium has been studied. A model of delayed hypersensitivity (DHS) to nonbacterial antigen in mice, a method of gel-filtration through the sephadex column G-200, immunosorbents were used. Three components with immunosuppressive activity: thermolabile component and thermostable one with direct immunosuppressive action and the third thermolabile component which manifested inductive immunosuppressive activity only after redox treatment have been revealed in the strain CFS.

[Electrophoretic patterns of cell wall protein as a criterion for the identification and classification of Corynebacteria].

Electrophoretic patterns of cell wall protein of three industrial strains, that were used for production of lysin, and eight collection strains from the genus Corynevacterium were studied to analyze their similarity as well as to estimate an opportunity of using this parameter as an additional criterion for identification and classification of corynebacteria. Similarity coefficient of cell wall overall and main protein electrophoretic patterns were determined by a specially created computer program. Electrophoretic analysis showed that every specie had an individual protein profile.

[DNA degradation in the leaves of the sugar beet (Beta vulgaris L.) in the process of senescence].

Programmed cell death (PCD) is a characteristic of all living beings in process of their development and in response to biotic and abiotic factors effects. On the pattern of the growing old 7-8th leaves of the sugar-beet (Beta vulgaris L.) of the first year vegetation of Belocerkovskaya singl-seed-45 sort character of the degradation and state of the methylation GTCGAC- and CTGCAG-sequences of the DNA were investigated.

[Structure and functional features of enzymes from the Ca2+-phospholipid-dependent protein kinase family].

Present day scientific data about the Ca(2+)-phospholipid-dependent protein kinases structure and molecular mechanisms of their activity are summarized and analyzed in this review. Ca(2+)-phospholipid-dependent protein kinases family is well known to include a whole series of enzymes which are homologous by their structure. They play an important role in cell differentiation, growth and proliferation as well as signal transduction through the cytoplasmic membrane.

[Effect of staphylococcus active substances on ATPase activity of smooth muscle actomyosin and myosin].

The effect of staphylococcus active substances--protein A (PA) and peptidoglican (PG) at concentrations 10(-6)-10(-2) mg/ml on the ATPase activity of pig stomach natural actomyosin and myosin was studied. It was shown that PA and PG at direct contact with smooth muscle contractile proteins caused the activation and inhibition of ATPase activity, respectively. On the basis of this investigation it was assumed that staphylococcal active substances were able to modify of the ATPase activity smooth muscle contractile proteins perhaps via direct action on the myosin molecule, which could be accompanied by conformational changes of the active center of myosin ATPase.

[Effect of C27-steroids on lipid peroxidation in rat liver mitochondria].

The influence of C27-steroids--vitamin D3 and 20-hydroxyecdysone in free-radical reactions on lipid peroxidation in the mitochondrial fraction of rat's liver has been studied. Antioxidant activity of superoxide dismutase and catalase and the content of lipid components--cholesterol and phospholipids has been determined. The assumption was put forward that antioxidant action of steroids was possible as a result of to the modification of mitochondrial membrane through its lipid content and changing of its cholesterol: phospholipids content.

[Principles of classifying and naming enzymes].

Common principles of enzyme classification, nomenclature and code recommended by the Committee on Enzyme of International Union of Biochemistry are described.

[Specific interaction of biological molecules. A new method of registration].

The experimental results on influence of some biological molecules and their specific immune complexes on the current-voltage characteristic of surface-barrier contact structures (Ni-Si or PorSiSi) are presented. It was shown that the deposition of the myoglobin and specific to its monoclonal antibodies or its specific immune complex caused the modification of the current-voltage characteristic. Moreover, the structures more essentially react to the formed specific immune complex deposition.

[Use of atomic force microscopy to study the morphology and structure of viruses].

The paper describes AFM methodic approaches in the investigation of viruses (TMV, phage T4). A possibility is shown to use this method to obtain high-quality three-dimensional image of objects without additional treatment with salts of heavy metals. The phage T4 was also a model to study the object density, using the phase shift of the AFM.

[The effect of antibiotic preparations and their combinations with probiotics on the intestinal microflora of mice].

Antibiotic drugs (biseptol, polymyxin, canamycin) as well as their combinations with probiotics biosporin and subalin have been studied for their effect on mice intestine microflora. It has been established that under peroral administration of the above drugs dysbacteriosis of the 2-3rd degrees arises in animals; under their combined use with probiotics one can prevent the development of dysbacteriosis to considerable extent. It has been shown that dysbacteriosis of the 2-3rd degrees can be liquidated for 5-7 days, using probiotics biosporin and subalin.