Inhibition of microRNA-9-3p reduces lipid accumulation in HepG2 cells by targeting the expression of sirtuin type 1.

Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder caused by the interaction of environmental factors and multiple genes. The genetic background of T2DM is complex and remains to be fully elucidated. MicroRNAs (miRNAs) are negative regulators of gene expression and several miRNAs are associated with the development of T2DM.

Correlation between microRNA-21 and expression of Th17 and Treg cells in microenvironment of rats with hepatocellular carcinoma.

Objective: To study the correlation between miR-21 and Treg/Th17 ratio in the microenvironment of rats with hepatocellular carcinoma.

Methods: Diethylnitrosamine was used to build the hepatocellular carcinoma model of rats; the content of Treg cells and Th17 cells and the expression of miR-21 in the peripheral blood of rats with hepatocellular carcinoma were detected. The statistical analysis was performed on the correlation between miR-21 expression and Treg/Th17 ratio.

Setup errors in cone-beam computed tomography and their effects on acute radiation toxicity in cervical cancer radiotherapy.

This study aimed to evaluate cone-beam computed tomography setup errors during cervical cancer treatment and the effects of these errors on acute radiation toxicity and treatment efficacy. A total of 170 cervical cancer patients were randomly divided into image-guided radiation therapy (IGRT; 86 patients) and intensity-modulated radiation therapy (IMRT; 84 patients) groups to receive IGRT and IMRT, respectively. After correcting setup errors for the 86 patients in the IGRT group, the X-, Y- and Z-axis errors were smaller than the corresponding errors before correction (P < 0.

Changes of TIZ expression in epithelial ovarian cancer cells.

Objective: To study the change of TIZ expression in epithelial ovarian cancer cells.

Methods: HO8910 cells were transinfected with siRNA to inhibit the expression of TIZ. pcDNA3.

Neuroprotective effects of brilliant blue G on the brain following traumatic brain injury in rats.

The P2X7 inhibitor, brilliant blue G (BBG), has been reported as a neuroprotective drug against a variety of disorders, including neuropathic pain and brain ischemia. Currently, no studies have examined the potential for BBG to provide neuroprotection in animal models of TBI. The aim of the present study was to investigate the neuroprotective effect of BBG on TBI and to determine the underlying mechanisms.

Clinical three-dimensional conformal radiotherapy research using repeated computed tomography scans for field reduction in older non-small-cell lung cancer patients.

This study investigated the curative and toxic effects of three-dimensional conformal radiotherapy (3D-CRT), using repeated CT scans for field reduction in older non-small-cell lung cancer (NSCLC) patients. 3D-CRT was administered to 36 older patients with NSCLC, and irradiation fields included the primary lesion and metastatic lymph nodes. After CT localization scanning, images were fed into a treatment planning system to delineate the gross tumor volume (GTV)1 and prepare Plan 1.

Protective effect of omeprazole on gastric mucosal of cirrhotic portal hypertension rats.

Objective: To observe the protective effect of omeprazole on gastric mucosal of cirrhotic portal hypertension rats.

Methods: All rats were randomly divided into normal control group, cirrhosis and treatment group. Thioacetamide was used to establish rat model of cirrhotic portal hypertension.

[Correlation between urinary stones and urinary tract infections].

Objective: To explore the correlation of urinary stones and urinary tract infections.

Methods: 300 cases with urinary tract stones received in our hospital from Feb. 2010 to Oct.

BTG1 expression in thyroid carcinoma: diagnostic indicator and prognostic marker.

We determined the expression and function of B cell translocation gene 1 (BTG1) in thyroid carcinoma. Thyroid samples were obtained from cancer lesions (n=83) and adjacent normal tissue (n=35) in thyroid cancer patients immediately after endoscopic biopsy. BTG1 expression was determined by immunohistochemistry and western blotting.

Association of EMP1 with gastric carcinoma invasion, survival and prognosis.

The aim of this study was to determine the expression and function of epithelial membrane protein 1 (EMP1) in gastric carcinoma. Gastric samples were taken from cancer lesions and adjacent normal tissue in gastric cancer patients immediately after endoscopic biopsy. A portion of the sample was either fixed in 4% paraformaldehyde and embedded in paraffin for immunohistochemistry or stored in liquid nitrogen for western blotting.

TPX2 overexpression in medullary thyroid carcinoma mediates TT cell proliferation.

TPX2 (targeting protein for xenopus kinesin-like protein 2), a microtubule-associated protein, plays an important role in the formation of the mitotic spindle. Abnormal expression of TPX2 in various types of malignant tumors has been reported, but less is known for medullary thyroid cancer (MTC). We investigated the expression of TPX2 in human MTC tissues and its potential use as a therapeutic target.

CCNG2 suppressor biological effects on thyroid cancer cell through promotion of CDK2 degradation.

This study aimed to analyze the expression and clinical significance of cyclin G2 (CCNG2) in thyroid carcinoma and the biological effects of CCNG2 overexpression in a cell line. Immunohistochemistry and Western blotting were used to analyze CCNG2 protein expression in 63 cases of thyroid cancer and normal tissues to allow the relationship with clinical factors to be assessed. CCNG2 lentiviral and empty vectors were transfected into the thyroid cancer K1 cell line.

BTG1 expression correlates with the pathogenesis and progression of breast carcinomas.

This study aimed to analyze the expression, clinical significance of B cell translocation gene 1 (BTG1) in breast carcinoma and the biological effect in its cell line by BTG1 overexpression. Immunohistochemistry and western blot were used to analyze BTG1 protein expression in 72 cases of breast cancer and 36 cases of normal tissues to study the relationship between BTG1 expression and clinical factors. Recombinant lentiviral vector was constructed to over-express EMP-1 and then infect breast cancer MCF-7 cell line.

Different association of manganese superoxide dismutase gene polymorphisms with risk of prostate, esophageal, and lung cancers: evidence from a meta-analysis of 20,025 subjects.

Altered expression or function of manganese superoxide dismutase (MnSOD) has been shown to be associated with cancer risk but assessment of gene polymorphisms has resulted in inconclusive data. Here a search of published data was made and 22 studies were recruited, covering 20,025 case and control subjects, for meta- analyses of the association of MnSOD polymorphisms with the risk of prostate, esophageal, and lung cancers. The data on 12 studies of prostate cancer (including 4,182 cases and 6,885 controls) showed a statistically significant association with the risk of development in co-dominant models and dominant models, but not in the recessive model.

[Relationship between clinical characteristics of lung cancer patients regarding their occupational exposure].

Objective: To study the relationship between clinical characteristics of lung cancer patients and their occupational exposure.

Methods: 2110 workers (≥ 20 years) who had been engaged in occupation that were exposed to risk factors of lung cancer, were asked to fill in questionnaires which included social demographic characteristics, length of service, smoking history and habits etc. 378 cases with lung cancer admitted in the Tangshan Worker's Hospital were selected and their clinical data including general information, symptoms or signs other than lungs, CT findings, histological type of the tumor, metastasis of tumors etc.

Clinical significance of mannose-binding lectin expression in thyroid carcinoma tissues.

Mannose-binding lectin (MBL) plays an important role in the host defence against pathogens and carcinogenesis. This study aimed to analyze differential expression of MBL protein in thyroid cancer tissues and then to investigate the effects of rhMBL in thyroid cancer cells. Tissue specimens from 45 thyroid carcinoma patients and 45 adenoma patients were recruited for immunohistochemical analysis of MBL expression.

Association between manganese superoxide dismutase gene polymorphism and breast cancer risk: a meta-analysis of 17,842 subjects.

The aim of this meta-analysis was to explore the association between the manganese superoxide dismutase (MnSOD) gene polymorphism and breast cancer risk, and to investigate the interaction of this gene polymorphism with known risk factors for breast cancer. Crude odds ratios (ORs) with 95% confidence intervals (CIs) for breast cancer risk associated with co-dominant models [valine/alanine (Val/Ala) vs. Val/Val, Ala/Ala vs.

[Variation and clinical significance of serum CTnT and CK-MB in patients with acute organophosphorus pesticid poisoning].

Aim: to explore the variation and clinical significance of serum CTnT and CK-MB in patients with acute organophosphorus pesticid poisoning (AOPP).

Methods: 100 cases of patients with AOPP(AOPP-group) and 100 healthy subjects were studied, the serum CTnT and CK-MB level were detected using ELISA and immunosuppression methods.

Results: after poisoning 6 h, 24h, 72h, the serum CTnT and CK-MB levels of the AOPP group were higher than the controls group, compared difference was significant (P<0.

[Study on the expression of uncoupling protein 2 in the kidney of the rats with hypothyroidism].

Objective: To observe the change in the expression of uncoupling protein 2 (UCP2) in kidney mitochondria in rats with hypothyroidism, and study the mechanism of renal injury due to hypothyroidism.

Methods: The Wistar rats were randomly divided into control group (n=10) and hypothyroidism group (n=10). The hypothyroidism rat model was reproduced by low-iodine diet.

Study on cognition disorder and morphologic change of neurons in hippocampus area following traumatic brain injury in rats.

Objective: To explore the correlation between cognition disorder and morphologic change of hippocampal neurons after traumatic brain injury (TBI).

Methods: Wistar rat models with severe TBI were made by Marmarou's method. The histopathological change of the neurons in the hippocampus area were studied with hematoxylin-eosin (HE) staining and terminal deoxynucleotidyl transferase-mediated X-dUPT nick end labeling (TUNEL), respectively.

[A study of the relationship between dynamic blood rheologic changes and the fluid resuscitation in burn patients].

Objective: To further study the relationship between hemor rheologic changes and fluid resuscitation in patients with burn and treatment of anemia after burns.

Methods: 238 case of burn patients were divided into four groups. In which patients with major burn were allocated to group C and D according to different methods of fluid resuscitation and treatment of anemia.

[Study on urine ALb, serum and urine beta 2-m, IgG, and THP].

The urine ALb, serum and urine beta 2-m, IgG, and THP were serially determined with radioimmunoassay to assess the renal functions during various stages of burn in 52 patients with TBSA over 30% (mean +/- s 58.4% +/- 18.88%).