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9 results match your criteria: "Tamilnad Hospital[Affiliation]"
Russ J Immunol
July 1999
Medical Biotechnology Research and Development, Research Council-Tamilnad Hospital Academic Trust, Chennai, India.
Chicken egg yolk is an abundant, yet inexpensive source of polyclonal antibodies. However, removal of the contaminating lipids and lipoproteins from immunoglobulins of egg yolk (IgY) is considered to be a laborious task during the purification. Liposomal asialoGM1 was used as a model immunogen for raising polyclonal IgY and tested with various methodological approaches.
View Article and Find Full Text PDFJ Agric Food Chem
July 2000
Medical Biotechnology, Tamilnad Hospital Academic Trust, Cheran Nagar, Perumbakkam, Chennai 601 302, India.
A sensitive spectrophotometric assay has been developed for flavonoids based on their binding with molybdenum, antimony, or bismuth. Acetylation of the hydroxyl group of flavonoids abolished metal binding, thus suggesting a direct role of the hydroxyl groups. From a comparison of several related flavonoids differing in the position of hydroxyl substitutions, the hydroxyl group at position 3 was found to be an important requirement for the formation of a yellow complex.
View Article and Find Full Text PDFNutrition
February 2000
Tamilnad Hospital, India.
With the availability of an organized nutrition support team (NST), the use of enteral nutrition (EN) can be extended to patients who would have otherwise received parenteral nutrition (PN). Although the formulation and accuracy of nutrient intake seems easier with PN, it is accepted that EN is cost effective and advantageous and should be used whenever possible. We discuss three cases in which the NST was initially consulted to provide PN.
View Article and Find Full Text PDFIndian J Exp Biol
July 1999
University of Madras Research Council-Tamilnad Hospital Academic Trust, Cheran Nagar, Perumbakkam, Chennai, India.
A egg yolk polyclonal IgY has been prepared by immunization of white leghorn chickens with small unilamellar liposomal asialoGM1. The newly prepared anti-asialoGM1 IgY has been characterized to be specific toward the terminal carbohydrate moiety of asialoGM1, and has no cross reactivity to its sialylated counterpart (ganglioside, GM1) as evidenced by immunochromatographic studies. General glycohistochemical methods along with antigen specific lectin and immunohistochemical staining using anti-asialoGM1 IgY were used to study the expression of Thomsen-Friedenreich (T-) disaccharide antigen in human colorectal adenocarcinoma tissues.
View Article and Find Full Text PDFIndian J Clin Biochem
July 1999
Department of Medical Biotechnology, Affiliated to University of Madras, Tamilnad Hospital Academic Trust-Research Council, Cheran Nagar, Perumbakkam, 601302 Chennai, India.
A comparison of the absorbance, enzyme/substrate concentration, reaction efficiency and sensitivity has been made for enzyme-based clinical chemistry assays, using a conventional colorimeter versus a strip-microwell reader, in order to establish the value of ultra-microchemical procedure, with reaction volume 87 μl (light path length=0.25 cm). By utilizing commercial kits available for the quantitation of serum glucose, cholesterol and triglycerides, it has been established that the micro method is highly cost effective (9-30 fold), reproducible and sensitive.
View Article and Find Full Text PDFNutrition
June 1999
Tamilnad Hospital, Chennai, India.
JPEN J Parenter Enteral Nutr
February 1998
Department of Surgery, Tamilnad Hospital, Chennai, India.
Background: Obstruction of feeding tubes is a common mechanical complication associated with enteral feeding. Standard methods of flushing are not always effective.
Methods: This study was conducted with patients receiving enteral feeding via nasogastric, nasoenteral, gastrostomy, or jejunostomy tubes to determine if prophylactic use of pancreatic enzymes would maintain patency of feeding tubes.
Nutrition
May 1996
Department of Surgery, Tamilnad Hospital, Madras, India.
Nutrition
September 1995
Department of Surgery, Tamilnad Hospital, Madras, India.