Overexpression of RGPR-p117 reveals anticancer effects by regulating multiple signaling pathways in bone metastatic human breast cancer MDA-MB-231 cells.

The role of RGPR-p117, a transcription factor, which binds to the TTGGC motif in the promoter region of the regucalcin gene, in cell regulation remains to be investigated. This study elucidated whether RGPR-p117 regulates the activity of triple-negative human breast cancer MDA-MB-231 cells in vitro. The wild-type and RGPR-p117-overexpressing cancer cells were cultured in DMEM supplemented with fetal bovine serum.

Risk Factors for Falls in Community-Dwelling Older Adults During the Novel Coronavirus Pandemic in Japan: A Prospective Cohort Study.

This study aimed to test the hypothesis that knowledge derived from indirect assessments can be used to identify fall risk factors during a period of social distancing. A baseline survey of 1953 community-dwelling older adults was conducted in May 2020, with a follow-up survey performed in May 2023 to assess the situation 3 years later. In total, 339 individuals were followed from baseline to follow-up.

Comparison of muscle activities during bench press at different angles in beginners.

[Purpose] The bench press can strengthen targeted muscles, such as the pectoralis major, deltoid, and triceps brachii. This study examined differences in muscle activities during the bench press at different angles in beginners to collect basic data and evaluate the clinical effectiveness of the bench press. [Participants and Methods] The participants were 15 male students with <1 year of training experience.

GATA2 participates in protection against hypoxia-induced pulmonary vascular remodeling.

The vascular endothelium is vital for cardio-pulmonary homeostasis and, thus, plays a crucial role in preventing life-threatening lung diseases. The transcription factor GATA2 is essential for hematopoiesis and maintaining vascular integrity. Heterozygous mutations in GATA2 can lead to a primary immunodeficiency syndrome with pulmonary manifestations.

Development and validation of clinical prediction model for functional independence measure following stroke rehabilitation.

Objectives: To develop and internally validate a clinical prediction model that includes balance ability and nutritional indices for the motor-functional independence measure (M-FIM) at 90 days post-stroke stroke.

Materials And Methods: This retrospective, single-center study included 566 patients with stroke undergoing rehabilitation at our rehabilitation hospital. The primary outcome was the M-FIM score of >61 at 3 months post-strokes onset.

Association of walking and activities of daily living with muscle thickness of the trunk and lower extremity muscles in children with down syndrome.

Purpose: We examined the association of walking and activities of daily living(ADL) with trunk and lower extremity muscle thickness in children with Down syndrome (DS).

Methods: 27 children with DS participated in this study. Walking was assessed using measurements of the maximal walking speed and step length, while ADL was assessed using the Pediatric Evaluation of DisabilityInventory (PEDI).

Therapeutic drug monitoring of docetaxel administered for breast cancer in a patient receiving rifampicin and clarithromycin to treat nontuberculous mycobacteriosis: A case report.

Docetaxel is metabolized by cytochrome P450 3A4 (CYP3A4), and is transported by organic anion transporting peptides (OATPs) and ABCB1, and its blood concentration is known to affect the risk of some docetaxel-related adverse drug reactions (ADRs). Thus, the concomitant use of docetaxel with drugs that inhibit or induce these transporters or CYP3A4 requires careful attention. A 58-year-old woman was receiving clarithromycin (400 mg twice daily), rifampicin (450 mg once daily) and ethambutol (500 mg once daily) for nontuberculous mycobacteriosis.

Foods provided at shelters during a heavy rain disaster: comparison of weighed food records between different numbers of days.

Dietitians working at evacuation shelters conduct weighed food records (WFR) for multiple days for dietary assessment. Because the menus in evacuation shelters do not change much from day to day, this study examined whether 1- and 2-d WFR are sufficient for dietary assessment at shelters and identified dietary components that can influence the number of assessment days. Overall, twenty-six WFR were collected from ten shelters in Kumamoto Prefecture, and the amounts of energy; protein; vitamins B, B and C and salt were calculated.

Cigarette smoke-induced attenuation of the prostaglandin transporter SLCO2A1 expression through aryl hydrocarbon receptor.

SLCO2A1 is a prostaglandin transporter and contributes to regulating local concentration of an inflammatory mediator, PGE. Since we previously found that cigarette smoke extracts (CSE) reduced Slco2a1 mRNA expression in rat alveolar epithelial cells, the current study aimed to investigate the effect of CSE on human SLCO2A1 mRNA expression across cell lines from organs that are susceptible to tobacco smoking-induced inflammation. 5'-Flanking regions of SLCO2A1 up to 3673 bp upstream of the transcription start site (+1) was sub-cloned into a luciferase (LUC) expression vector, and promoter activity was evaluated by a reporter assay.

Comparison of the masses and amounts of intramuscular non-contractile tissue of the lower extremity muscles, mobility and balance ability, and cognitive function between older adults with and without low back pain: A pilot study.

Introduction: This study compared the masses and amounts of intramuscular non-contractile tissue of multiple lower extremity muscles measured using an ultrasound imaging device, as well as the mobility and balance ability and cognitive function between community-dwelling older adults with and without low back pain (LBP).

Methods: Twenty-five community-dwelling older adults were classified into control (CTR) (n = 17, asymptomatic) and LBP (n = 8) groups. The current LBP status in the LBP group was as follows: duration period: 99.

Neuron-selective and activity-dependent splicing of BDNF exon I-IX pre-mRNA.

Brain-derived neurotrophic factor (BDNF) is essential for numerous neuronal functions, including learning and memory. The expression of BDNF is regulated by distinctive transcriptional and post-transcriptional mechanisms. The Bdnf gene in mice and rats comprises eight untranslated exons (exons I-VIII) and one exon (exon IX) that contains the pre-proBDNF coding sequence.

Panax notoginseng root extract induces nuclear translocation of CRTC1 and Bdnf mRNA expression in cortical neurons.

Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, is deeply involved in the development and higher function of the nervous system, including learning and memory. By contrast, a reduction in BDNF levels is associated with various neurological disorders such as dementia and depression. Therefore, the inducers of Bdnf expression might be valuable in ameliorating or protecting against a decline in brain functions.

Attitudinal changes of undergraduate students learning online interprofessional education for patient safety: Comparative evaluation of an online program using the DID method.

Objective: Interprofessional education (IPE) can cultivate competencies in multidisciplinary collaboration for patient safety, and both face-to-face and online IPE programs have recently been introduced. This study aimed to elucidate the effects of the online IPE program on undergraduate students after the coronavirus disease 2019 pandemic.

Methods: The difference-in-differences method was used to assess undergraduate students in the Schools of Medicine and Health Sciences and in the Faculty of Pharmacy at Takasaki University of Health and Welfare who participated in IPE programs at Gunma University (face-to-face IPE was implemented in 2019 and online IPE in 2020).

Reliability of motion phase identification for long-track speed skating using inertial measurement units.

Background: Precise identification of motion phases in long-track speed skating is critical to characterize and optimize performance. This study aimed to estimate the intra- and inter-rater reliability of movement phase identification using inertial measurement units (IMUs) in long-track speed skating.

Methods: We analyzed 15 skaters using IMUs attached to specific body locations during a 500m skate, focusing on the stance phase, and identifying three movement events: Onset, Edge-flip, and Push-off.

Direct protein delivery into intact Arabidopsis cells for genome engineering.

Intracellular delivery of biomolecules is a prerequisite for genetic techniques such as recombinant engineering and genome editing. Realizing the full potential of this technology requires the development of safe and effective methods for delivering protein tools into cells. In this study, we demonstrated the spontaneous internalization of exogenous proteins into intact cells and root tissue of whole plants of Arabidopsis thaliana.

Accelerated BDNF expression in visceral white adipose tissues following high-fat diet feeding in mice.

Brain-derived neurotrophic factor (BDNF) is expressed in the white adipose tissues (WATs), and the expression increases during high-fat diet (HFD) feeding, implicating its role in obesity. Here, we focused on BDNF expression in epididymal WAT (eWAT), a visceral adipose tissue, in mice. During 2 weeks of HFD feeding, Bdnf mRNA expression in eWAT slightly increased, but a robust increase was observed after 8 weeks of HFD feeding.

High disease activity influences the presence of vertebral fractures in rheumatoid arthritis.

Introduction: It is important to assess the risk of vertebral fractures (VFs) in patients with rheumatoid arthritis (RA), as RA is associated with a high risk of VFs. However, the epidemiology and risk of VFs in patients with RA remain inconclusive. The present study therefore clarified the prevalence and associated factors of VFs in patients with RA.

Endothelial GATA3 is involved in coagulofibrinolytic homeostasis during endotoxin sepsis.

Sepsis-induced acute lung injury represents a significant threat to human health and is frequently associated with pulmonary thrombosis due to dysregulation of the coagulofibrinolytic system. Plasmin, the major protease that degrades fibrin aggregates, is activated predominantly by tissue-plasminogen activator (tPA), whereas tPA is negatively regulated by plasminogen activator inhibitor (PAI-1). Under septic conditions, the imbalance between coagulation and fibrinolysis results in excessive microthrombosis.

Inhibition of 15-prostaglandin dehydrogenase attenuates acetaminophen-induced liver injury via suppression of apoptosis in liver endothelial cells.

Hepatic microvascular disruption caused by injury to liver sinusoidal endothelial cells (LSECs) is an aggravating factor for drug-induced liver injury (DILI). It is suggested that prostaglandin E (PGE) may be able to attenuate LSEC injury. However, it is also known that 15-keto PGE, a metabolite of PGE produced by 15-prostaglandin dehydrogenase (15-PGDH) that is not a ligand of PGE receptors, suppresses inflammatory acute liver injury as a ligand of peroxisome proliferator-activated receptor γ.

Patient resuscitated after cardiopulmonary arrest exhibits abnormally increased phenytoin metabolic rate due to unknown factors: a case report.

Background: Fosphenytoin (FOS) is a prodrug of phenytoin (PHT) with a metabolism that exhibits Michaelis-Menten-type kinetics. Genetic polymorphisms of the metabolic enzymes of PHT make it challenging to predict its plasma concentrations. High plasma PHT concentrations are typically problematic, and several causes have been elucidated.