Whole-transcriptome sequencing with ceRNA regulation network construction and verification in glioblastoma.

Objectives: To explore the key genes involved in the occurrence and development of glioblastoma (GBM) by analyzing whole-transcriptome sequencing and biologic data from GBM and normal cerebral cortex tissues and to search for important noncoding RNA (ncRNA) molecular markers based on the competitive endogenous RNA (ceRNA) network.

Methods: Ten GBM and normal cerebral cortex tissues were collected for full transcriptome sequencing, screened for differentially expressed (DE) mRNAs, miRNAs, lncRNAs, and circRNAs, and subjected to bioinformatic analysis. We constructed a Protein-Protein Interaction (PPI) network and a circRNA/lncRNA-miRNA-mRNA regulatory network and identified them using reverse transcription-quantitative polymerase chain reaction (RT-qPCR).

Pd -Catalyzed Asymmetric Carbonitratation Reaction Featuring an H-Bonding-Driven Alkyl-Pd -ONO Reductive Elimination.

Reductive elimination of alkyl-Pd -O is a synthetically useful yet underdeveloped elementary reaction. Here we report that the combination of an H-bonding donor [PyH][BF ] and AgNO additive under toluene/H O biphasic system can enable such elementary step to form alkyl nitrate. This results in the Pd -catalyzed asymmetric carbonitratations of (Z)-1-iodo-1,6-dienes with (R)-BINAP as the chiral ligand, affording alkyl nitrates up to 96 % ee.

A Cu-doped two-dimensional material-based heterojunction photoelectrode: application for highly sensitive photoelectrochemical detection of hydrogen sulfide.

In this work, on the basis of a Cu-doped two-dimensional material-based heterojunction photoelectrode, a novel anodic photoelectrochemical (PEC) sensing platform was constructed for highly sensitive detection of endogenous HS. Briefly, with g-CN and TiO as representative materials, the sensor was fabricated by modifying g-CN/TiO nanorod arrays (NAs) onto the surface of fluorine-doped tin oxide (FTO) and then doping Cu as a Cu S ( = 1, 2) precursor. After the binding of S with surface-attached Cu, the signal was quenched owing to the generation of Cu S which offers trapping sites to hinder generation of photocurrent signals.