Cyclic mechanical stress promotes osteogenic differentiation of periodontal ligament stem cells via KAT2A-mediated succinylation of WDR5.

Orthodontics promotes tooth movement and periodontal reconstruction by regulating osteogenic differentiation and osteoclast resorption. This study aimed to investigate the effect of orthodontic force during orthodontics on osteogenesis of periodontal ligament stem cells (PDLSCs) and the underlying mechanism. Cyclic mechanical stress was applied on PDLSCs, and osteogenic differentiation was analyzed using quantitative real-time polymerase chain reaction, immunoblotting, alkaline phosphatase (ALP) staining, and ALP activity determination.