Transformed phenotype of enzootic bovine lymphoma reflects differentiation-linked leukemogenesis.

Detailed genotypic and phenotypic characteristics of enzootic bovine lymphoma were defined to determine if tumor cell characteristics were consistent with the etiologic agent of this disease. Lymphoid tumors from cattle with serologic evidence of bovine leukemia virus (BLV) infection were examined by Southern blot analysis to confirm the presence and copy number of integrated BLV. All cases of enzootic bovine lymphoma examined contained 1 to 4 BLV proviral copies in tumor DNA and revealed immunoglobulin gene rearrangement (Ig).

Flow cytometric detection of beta-globin mRNA in murine haemopoietic tissues using fluorescent in situ hybridization.

The novel method for flow cytometric detection of cellular RNA species in suspended cells by fluorescent in situ hybridization (FC-FISH) was applied in the evaluation of beta-globin expression in murine haemopoietic tissues. Normal murine bone marrow cells and regenerating bone marrow cells obtained after lethal irradiation and bone marrow transplantation as well as murine 15 d fetal liver were examined. Furthermore, spleens and bone marrow of phenylhydrazine-induced anaemic mice were studied.