Soil fertilization with microalgae biomass from municipal wastewater treatment causes no additional leaching of dissolved macronutrients and trace elements in a column experiment.

Microalgae are a promising bio-fertilizer that can be cultivated in municipal wastewater, where the organisms perform water purification by incorporation of nutrients and contaminants. Before bio-fertilization with wastewater-grown microalgae can be put into practice, its impact on the leaching of macronutrients and trace elements needs to be evaluated. Here, we studied the leaching behavior of a microalgae-fertilized soil against a control in column percolation setup.

The Schottky barrier transistor in emerging electronic devices.

This paper explores how the Schottky barrier (SB) transistor is used in a variety of applications and material systems. A discussion of SB formation, current transport processes, and an overview of modeling are first considered. Three discussions follow, which detail the role of SB transistors in high performance, ubiquitous and cryogenic electronics.

Post-measurement compressed calibration for ICP-MS-based metal quantification in mine residues bioleaching.

Bioleaching is an actual economical alternative to treat residues, which allows, depending on the chosen strategy, two possible outcomes: (1) a leachate enriched with target metals, or (2) a residue enriched in target metals through the leaching of interfering components (IC). This work aimed to study the metals released by bioprocessing the Panasqueira mine tailings, as a strategy to increase critical metals' relative concentration in residues. Biostimulation of the local microbiota was compared to a bioaugmentation approach using the autochthonous Diaphorobacter polyhydroxybutyrativorans strain B2A2W2.

Comparability of Heart Rate Turbulence Methodology: 15 Intervals Suffice to Calculate Turbulence Slope - A Methodological Analysis Using PhysioNet Data of 1074 Patients.

Heart rate turbulence (HRT) is a characteristic heart rate pattern triggered by a ventricular premature contraction (VPC). It can be used to assess autonomic function and health risk for various conditions, e.g.

Process intensification for the continuous production of an antimicrobial peptide in stably-transformed Sf-9 insect cells.

The antibiotic resistance crisis has prompted research into alternative candidates such as antimicrobial peptides (AMPs). However, the demand for such molecules can only be met by continuous production processes, which achieve high product yields and offer compatibility with the Quality-by-Design initiative by implementing process analytical technologies such as turbidimetry and dielectric spectroscopy. We developed batch and perfusion processes at the 2-L scale for the production of BR033, a cecropin-like AMP from Lucilia sericata, in stably-transformed polyclonal Sf-9 cells.

The potential impact of an implementation of microalgae-based wastewater treatment on the energy balance of a municipal wastewater treatment plant in Central europe.

Integration of a photobioreactor for WWT by microalgae is calculated as a future alternative for cost-efficient and environmentally-friendly nutrient removal for municipal WWTPs. High growth rates and higher biogas yields (compared to conventional sewage sludge) of algal biomass can significantly improve WWTP energy balances. This study focuses on temperate climate zones with changing seasons and discusses energy potential of microalgae-enhanced wastewater treatment for an existing WWTP (32,000 PE) in Central Germany.

Countering reproducibility issues in mathematical models with software engineering techniques: A case study using a one-dimensional mathematical model of the atrioventricular node.

One should assume that in silico experiments in systems biology are less susceptible to reproducibility issues than their wet-lab counterparts, because they are free from natural biological variations and their environment can be fully controlled. However, recent studies show that only half of the published mathematical models of biological systems can be reproduced without substantial effort. In this article we examine the potential causes for failed or cumbersome reproductions in a case study of a one-dimensional mathematical model of the atrioventricular node, which took us four months to reproduce.

Low Heart Rate Variability Predicts Stroke and Other Complications in the First Six Postoperative Months After a Hip Fracture Operation.

One-year mortality after hip fractures is underestimated and is reported as 25%. An improved risk stratifying could contribute to a better follow up of these patients. Heart Rate Variability (HRV) is an easy point-of-care investigation and is been used in cardiology, endocrinology, and perioperative care.

Full-scale accelerated carbonation of waste incinerator bottom ash under continuous-feed conditions.

Bottom ash (BA) is the dominant residue derived from the incineration of municipal solid waste or refuse-derived fuel (RDF). Costs for the disposal of the material chiefly depend on the leachability of salts and trace metals which may be cut by ageing the BA for several months to promote carbonation via uptake of carbon dioxide (CO). Enhanced exposure to CO sources has been referred to as accelerated carbonation.

NeuroKit2: A Python toolbox for neurophysiological signal processing.

NeuroKit2 is an open-source, community-driven, and user-centered Python package for neurophysiological signal processing. It provides a comprehensive suite of processing routines for a variety of bodily signals (e.g.

Technical note: Vendor-agnostic water phantom for 3D dosimetry of complex fields in particle therapy.

Purpose: Three-dimensional (3D) dosimetry is a necessity to validate patient-specific treatment plans in particle therapy as well as to facilitate the development of novel treatment modalities. Therefore, a vendor-agnostic water phantom was developed and verified to measure high resolution 3D dose distributions.

Methods: The system was experimentally validated at the Marburger Ionenstrahl-Therapiezentrum using two ionization chamber array detectors (PTW Octavius 1500XDR and 1000P) with 150.

Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells.

The discovery of the genome-editing tool CRISPR-Cas9 is revolutionizing the world of gene therapy and will extend the gene therapy product pipeline. While applying gene therapy products, the main difficulty is an efficient and effective transfer of the nucleic acids carrying the relevant information to their target destination, the nucleus of the cells. Baculoviruses have shown to be very suitable transport vehicles for this task due to, inter alia, their ability to transduce mammalian/human cells without being pathogenic.

Upstream and Downstream Processes for Viral Nanoplexes as Vaccines.

The increasing medical interest in viral nanoplexes, such as viruses or virus-like particles used for vaccines, gene therapy products, or oncolytic agents, raises the need for fast and efficient production processes. In general, these processes comprise upstream and downstream processing. For the upstream process, efficiency is mainly characterized by robustly achieving high titer yields, while reducing process times and costs with regard to the cell culture medium, the host cell selection, and the applied process conditions.

Bioreactor-Based Antigen Production Process Using the Baculovirus Expression Vector System.

Several vaccines are already produced using the baculovirus expression vector system (BEVS). This chapter describes methods for generating recombinant baculoviral DNA (also called bacmid) for cultivating Spodoptera frugiperda Sf-9 cells and producing a baculovirus stock from the recombinant bacmid and for producing a protein-based vaccine with the BEVS in a stirred tank reactor.

HRT assessment reviewed: a systematic review of heart rate turbulence methodology.

Unlabelled: Heart rate turbulence (HRT) is a biphasic reaction to a ventricular premature contraction (VPC) mainly mediated by the baroreflex. It can be used for risk stratification in different disease patterns. Despite existing standards there is a lot of variation in terms of measuring and calculating HRT, which complicates research and application.

Levan-based hydrogels for controlled release of Amphotericin B for dermal local antifungal therapy of Candidiasis.

Hydrogels from Halomonas levan polysaccharide were prepared at different crosslinking densities. Swelling results demonstrated pH dependent rather than temperature dependent swelling of the hydrogel and the highest swelling value was achieved at basic conditions with a swelling ratio of 9.1 ± 0.

Turbidimetry and Dielectric Spectroscopy as Process Analytical Technologies for Mammalian and Insect Cell Cultures.

The production of biopharmaceuticals in cell culture involves stringent controls to ensure product safety and quality. To meet these requirements, quality by design principles must be applied during the development of cell culture processes so that quality is built into the product by understanding the manufacturing process. One key aspect is process analytical technology, in which comprehensive online monitoring is used to identify and control critical process parameters that affect critical quality attributes such as the product titer and purity.

Measurement of PET isotope production cross sections for protons and carbon ions on carbon and oxygen targets for applications in particle therapy range verification.

Measured cross sections for the production of the PET isotopes [Formula: see text], [Formula: see text] and [Formula: see text] from carbon and oxygen targets induced by protons (40-220 [Formula: see text]) and carbon ions (65-430 [Formula: see text]) are presented. These data were obtained via activation measurements of irradiated graphite and beryllium oxide targets using a set of three scintillators coupled by a coincidence logic. The measured cross sections are relevant for the PET particle range verification method where accurate predictions of the [Formula: see text] emitter distribution produced by therapeutic beams in the patient tissue are required.

Continuous-feed carbonation of waste incinerator bottom ash in a rotating drum reactor.

Carbonation is a key process in the aging of waste incinerator bottom ash (BA). The reaction with CO decreases the BA alkalinity and lowers the leachability of amphoteric trace metals. Passive ageing over several months is usually performed in intermittently mixed BA heaps.

Experimental Assessment of Lithium Hydride's Space Radiation Shielding Performance and Monte Carlo Benchmarking.

The harmful effects of space radiation pose a serious health risk to astronauts participating in future long-term missions. Such radiation effects must be considered in the design phase of space vessels as well as in mission planning. Crew radioprotection during long periods in deep space (e.

Towards optimized drum composting: evaluation of the radial mixing performance of a model substrate on the laboratory scale.

The rotating drum composter (RDC) is one of the most widespread reactor systems for biowaste treatment, worldwide. Nevertheless, knowledge on optimum operating conditions including, e.g.

Effectiveness of a new radiation protection system in the interventional radiology setting.

Objectives: The goal of this study was to examine a new weightless-like radiation protection garment regarding its radiation protection efficacy and to compare it to a conventional two-piece apron suit plus thyroid collar and standard ancillary shields.

Material And Methods: All measurements were carried out using a clinical angiography system with a standardized fluoroscopy protocol for different C-arm angulations. An anthropomorphic torso phantom served as a scattering body.

A fast and simple method to monitor carbonation of MSWI bottom ash under static and dynamic conditions.

Accelerated carbonation may be employed to improve the leaching behaviour and the geotechnical properties of MSWI bottom ash (BA). Here we report on a novel method to monitor and evaluate the progress of carbonation in both static and dynamic reactor systems. The method is based on following the pressure drop in the gas phase induced by the CO-uptake of BA and was benchmarked against carbonate contents as measured by thermogravimetry.

Odd and disorganized-Comparing the factor structure of the three major schizotypy inventories.

Schizotypy is a personality-framework currently considered a comprehensive and useful construct in schizophrenia research and becoming ever more accepted within psychiatry. Thus, it is crucial to obtain a unifying and generally accepted means of its measuring. The three most commonly used psychometric schizotypy inventories, the Wisconsin Schizotypy Scales, Schizotypal Personality Questionnaire and Oxford-Liverpool Inventory of Feelings and Experiences were, however, built upon different theoretical schizotypy models, wherefore comparing results from studies that have used different inventories is difficult and sometimes questionable.