6 results match your criteria: "Sydney University at Royal North Shore Hospital[Affiliation]"
Placenta
February 2003
Department of Renal Medicine, Sydney University at Royal North Shore Hospital, St Leonards, NSW, Australia.
We and others have previously observed an imbalance in cytotrophoblast secretion of the vasoactive prostanoids prostacyclin and thromboxane A(2) in pre-eclampsia. To examine the effects of potential modulators of this imbalance, cytotrophoblasts isolated from normal and pre-eclamptic pregnancies were incubated in the presence of lipopolysaccharide, the calcium ionophore A23187, tumour necrosis factor alpha, or interleukin 1beta, with or without the cyclo-oxygenase inhibitor, indomethacin. Further incubations included the drugs tranylcypromine, a prostacyclin synthetase inhibitor (0.
View Article and Find Full Text PDFBJOG
June 2001
Department of Renal Medicine, Sydney University at Royal North Shore Hospital, St Leonards, NSW, Australia.
Investigation of the pathophysiology of pre-eclampsia (characterised by insufficient invasion of the intrauterine vasculature by cytotrophoblasts) has been hampered by the absence of a suitable animal model, and ethical constraints in clinical studies. We have developed a novel in vitro human cell co-culture system allowing direct assessment of cytotrophoblast invasion of a decidual endothelial cell monolayer from the abluminal side, as occurs in vivo. This model will facilitate detection, at the cellular level, of abnormal endothelial cell-trophoblast functional interactions in pre-eclampsia and other pregnancy disorders with abnormal placentation.
View Article and Find Full Text PDFJ Soc Gynecol Investig
May 2001
Department of Renal Medicine, Sydney University at Royal North Shore Hospital, St Leonards, 2065, New South Wales, Australia.
Objective: To examine the effect of plasma from preeclamptic women on production of the vasoactive substances prostacyclin, thromboxane, nitric oxide, and cyclic guanosine monophosphate (cGMP) by decidual endothelial cells; to determine any effects on cell growth and health; and to examine whether cells from preeclamptic women are activated compared with cells from normal women.
Methods: Decidual endothelial cells from normal and preeclamptic women were incubated for 24 hours in media containing 10% plasma from preeclamptic women or matched normal women. Prostacyclin and thromboxane production was measured, as was nitric oxide and cGMP production after a further 45-minute generation period in 2% test plasma.
J Soc Gynecol Investig
July 2000
Department of Renal Medicine, Sydney University at Royal North Shore Hospital, St. Leonards, New South Wales, Australia.
Objectives: To examine the effect on prostacyclin and thromboxane production of incubating decidual endothelial cells under hypoxic conditions, comparing cells from normal and preeclamptic pregnancies. Furthermore, to determine whether hypoxia is deleterious to cell growth, and test the effects of stimulation and inhibition on prostanoid production.
Methods: Decidual endothelial cells were exposed for a total of 40 hours to normoxic (20% O2) or hypoxic (2% O2) conditions.
Baillieres Best Pract Res Clin Obstet Gynaecol
March 1999
Department of Renal Medicine, Sydney University at Royal North Shore Hospital, New South Wales, Australia.
Hypertension is a relatively common complication of pregnancy, increasing in frequency in older women. It is not a contraindication to pregnancy, but should be fully investigated, correctable causes addressed and those with specific relevance for pregnancy identified. With close supervision and appropriate management, the majority of hypertensive pregnant women have successful outcomes.
View Article and Find Full Text PDFHum Reprod
January 1997
Department of Psychological Medicine, Sydney University at Royal North Shore Hospital, Australia.
The aim of this study was to compare 70 couples who had conceived by in-vitro fertilization (IVF) with 63 matched controls for the prevalence of anxiety and quality of attachment to the baby during pregnancy. Results for mothers showed no group differences using a global measure of anxiety, the Spielberger State-Trait Anxiety Inventory. However, pregnancy-specific measures revealed significantly higher levels of anxiety in IVF mothers about the survival and normality of their unborn babies, about damage to their babies during childbirth and about separating from their babies after birth.
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