Determination of Ventilatory Thresholds Using Near-Infrared Spectroscopy in Recreational Endurance and CrossFit Athletes.

To define training zones, ventilatory thresholds (VTs) are commonly established by cardiopulmonary gas-exchange analysis during incremental exercise tests. Portable near-infrared spectroscopy (NIRS) devices have emerged as a potential tool for detecting these thresholds by monitoring muscle oxygenation. This study evaluated the accuracy of NIRS measurements to determine VTs or critical power (CP) based on muscle oxygen saturation and assesses the device's consistency across 2 constant-load tests.

Effect of soil-groundwater system on migration and transformation of organochlorine pesticides: A review.

Soil is the place where human beings, plants, and animals depend on for their survival and the link between the various ecological layers. Groundwater is an important component of water resources and is one of the most important sources of water for irrigated agriculture, industry, mining and cities because of its stable quantity and quality. Soil and groundwater are important strategic resources highly valued by countries around the world.

Nutrient colimitation is a quantitative, dynamic property of microbial populations.

Resource availability dictates how fast and how much microbial populations grow. Quantifying the relationship between microbial growth and resource concentrations makes it possible to promote, inhibit, and predict microbial activity. Microbes require many resources, including macronutrients (e.

Lactate dehydrogenase B noncanonically promotes ferroptosis defense in KRAS-driven lung cancer.

Ferroptosis is an oxidative, non-apoptotic cell death frequently inactivated in cancer, but the underlying mechanisms in oncogene-specific tumors remain poorly understood. Here, we discover that lactate dehydrogenase (LDH) B, but not the closely related LDHA, subunits of active LDH with a known function in glycolysis, noncanonically promotes ferroptosis defense in KRAS-driven lung cancer. Using murine models and human-derived tumor cell lines, we show that LDHB silencing impairs glutathione (GSH) levels and sensitizes cancer cells to blockade of either GSH biosynthesis or utilization by unleashing KRAS-specific, ferroptosis-catalyzed metabolic synthetic lethality, culminating in increased glutamine metabolism, oxidative phosphorylation (OXPHOS) and mitochondrial reactive oxygen species (mitoROS).

Soil carbon in the world's tidal marshes.

Tidal marshes are threatened coastal ecosystems known for their capacity to store large amounts of carbon in their water-logged soils. Accurate quantification and mapping of global tidal marshes soil organic carbon (SOC) stocks is of considerable value to conservation efforts. Here, we used training data from 3710 unique locations, landscape-level environmental drivers and a global tidal marsh extent map to produce a global, spatially explicit map of SOC storage in tidal marshes at 30 m resolution.

A single-chain variable fragment-based bispecific T-cell activating antibody against CD117 enables T-cell mediated lysis of acute myeloid leukemia and hematopoietic stem and progenitor cells.

Acute myeloid leukemia (AML) derives from hematopoietic stem and progenitor cells (HSPCs). To date, no AML-exclusive, non-HSPC-expressed cell-surface target molecules for AML selective immunotherapy have been identified. Therefore, to still apply surface-directed immunotherapy in this disease setting, time-limited combined immune-targeting of AML cells and healthy HSPCs, followed by hematopoietic stem cell transplantation (HSCT), might be a viable therapeutic approach.

New insights into the stages of cadmium remediation in ryegrass enhanced by kitchen compost-derived dissolved organic matter: Activation, absorption, and storage.

Dissolved organic matter (DOM) regulates plant behavior in both agricultural and environmental fields. However, the regulatory mechanisms by which DOM influences soil-plant system interactions during the phytoremediation of Cd-contaminated soils remain unclear. Therefore, this study investigated the enhanced effect of kitchen compost-derived DOM on the Cd remediation capability of ryegrass across three phases of phytoremediation.

Light-Activated Agonist-Potentiator of GABA Receptors for Reversible Neuroinhibition in Wildtype Mice.

Gamma aminobutyric acid type A receptors (GABARs) play a key role in the mammalian central nervous system (CNS) as drivers of neuroinhibitory circuits, which are commonly targeted for therapeutic purposes with potentiator drugs. However, due to their widespread expression and strong inhibitory action, systemic pharmaceutical potentiation of GABARs inevitably causes adverse effects regardless of the drug selectivity. Therefore, therapeutic guidelines must often limit or exclude clinically available GABAR potentiators, despite their high efficacy, good biodistribution, and favorable molecular properties.

Applying kitchen compost promoted soil chrysene degradation by optimizing microbial community structure.

Chrysene, as a high molecular weight polycyclic aromatic hydrocarbon (PAH), has become an important factor in degrading soil quality and constraining the safe production of food crops. Compost has been widely used to amend contaminated soil. However, to date, the main components of kitchen compost that enhance the biodegradation of chrysene in the soil remain unidentified.

A role for leucine-rich, glioma inactivated 1 in regulating pain sensitivity.

Neuronal hyperexcitability is a key driver of persistent pain states including neuropathic pain. Leucine-rich, glioma inactivated 1 (LGI1), is a secreted protein known to regulate excitability within the nervous system and is the target of autoantibodies from neuropathic pain patients. Therapies that block or reduce antibody levels are effective at relieving pain in these patients, suggesting that LGI1 has an important role in clinical pain.

Cortical oscillations are modified by expertise in dance and music: Evidence from live dance audience.

Over the past decades, the focus of brain research has expanded from using strictly controlled stimuli towards understanding brain functioning in complex naturalistic contexts. Interest has increased in measuring brain processes in natural interaction, including classrooms, theatres, concerts and museums to understand the brain functions in the real world. Here, we examined how watching a live dance performance with music in a real-world dance performance setting engages the brains of the spectators.

Interactions between microplastics and heavy metals in leachate: Implications for landfill stabilization process.

The emission of microplastics and heavy metals in landfills has attracted widespread attention for its stabilization process. Microplastics have become carriers of heavy metals due to their adsorption properties, affecting their environmental behavior. However, the effects of landfill stabilization on the interaction between microplastics and heavy metals in leachate are ambiguous.

Tumor-Homing Antibody-Cytokine Fusions for Cancer Therapy.

Recombinant cytokine products have emerged as a promising avenue in cancer therapy due to their capacity to modulate and enhance the immune response against tumors. However, their clinical application is significantly hindered by systemic toxicities already at low doses, thus preventing escalation to therapeutically active regimens. One promising approach to overcoming these limitations is using antibody-cytokine fusion proteins (also called immunocytokines).

Fragmented micro-growth habitats present opportunities for alternative competitive outcomes.

Bacteria in nature often thrive in fragmented environments, like soil pores, plant roots or plant leaves, leading to smaller isolated habitats, shared with fewer species. This spatial fragmentation can significantly influence bacterial interactions, affecting overall community diversity. To investigate this, we contrast paired bacterial growth in tiny picoliter droplets (1-3 cells per 35 pL up to 3-8 cells per species in 268 pL) with larger, uniform liquid cultures (about 2 million cells per 140 µl).

Tripokin: A multi-specific immunocytokine for cancer immunotherapy.

Antibodies that target the tumor microenvironment can be used to deliver pro-inflammatory payloads, such as cytokines. Cytokines are small proteins able to modulate the activity of the immune system, and antibody-cytokine fusion proteins have been tested in preclinical and clinical settings. In this study, we describe Tripokin, a novel multi-specific fusion protein that combines interleukin-2 and a single amino acid mutant of tumor necrosis factor.

Network medicine-based epistasis detection in complex diseases: ready for quantum computing.

Most heritable diseases are polygenic. To comprehend the underlying genetic architecture, it is crucial to discover the clinically relevant epistatic interactions (EIs) between genomic single nucleotide polymorphisms (SNPs) (1-3). Existing statistical computational methods for EI detection are mostly limited to pairs of SNPs due to the combinatorial explosion of higher-order EIs.

Temporal and spatial frameworks supporting plant responses to vegetation proximity.

After the perception of vegetation proximity by phytochrome photoreceptors, shade-avoider plants initiate a set of responses known as the shade avoidance syndrome (SAS). Shade perception by the phytochrome B (phyB) photoreceptor unleashes the PHYTOCHROME INTERACTING FACTORs and initiates SAS responses. In Arabidopsis (Arabidopsis thaliana) seedlings, shade perception involves rapid and massive changes in gene expression, increases auxin production, and promotes hypocotyl elongation.

γ1 GABA Receptors in Spinal Nociceptive Circuits.

GABAergic neurons and GABA receptors (GABARs) are critical elements of almost all neuronal circuits. Most GABARs of the CNS are heteropentameric ion channels composed of two α, two β, and one γ subunits. These receptors serve as important drug targets for benzodiazepine (BDZ) site agonists, which potentiate the action of GABA at GABARs.

Removal of TREX1 activity enhances CRISPR-Cas9-mediated homologous recombination.

CRISPR-Cas9-mediated homology-directed repair (HDR) can introduce desired mutations at targeted genomic sites, but achieving high efficiencies is a major hurdle in many cell types, including cells deficient in DNA repair activity. In this study, we used genome-wide screening in Fanconi anemia patient lymphoblastic cell lines to uncover suppressors of CRISPR-Cas9-mediated HDR. We found that a single exonuclease, TREX1, reduces HDR efficiency when the repair template is a single-stranded or linearized double-stranded DNA.