Identifying non-responders to cardiac resynchronization therapy in the non-left bundle branch block.

Background: Non-response to cardiac resynchronization therapy (CRT) is an important issue in the treatment of heart failure with reduced ejection fraction (HFrEF) and non-left bundle branch block (LBBB). Electrocardiogram-gated myocardial perfusion single-photon emission computed tomography imaging (G-MPI SPECT) is typically used to assess left ventricular (LV) dyssynchrony. This study aimed to determine whether G-MPI parameters are associated with non-responsiveness to CRT.

Characterization of a Novel Col1a1 Osteogenesis Imperfecta Mouse Model with Insights into Skeletal Phenotype, Fragility, and Therapeutic Evaluations.

Osteogenesis imperfecta (OI) is an inheritable skeletal disorder characterized by bone fragility often caused by pathogenic variants in the COL1A1 gene. Current OI mouse models with a glycine substitution in Col1a1 exhibit excessive severity, thereby limiting long-term pathophysiological analysis and drug effect assessments. To address this limitation, we constructed a novel OI mouse model mimicking a patient with OI type III.

Basic Science and Pathogenesis.

Background: In aging societies, neurodegenerative diseases, such as Alzheimer's disease, are receiving attention. These diseases are primary targets for preemptive medicine, emphasizing the importance of early detection and preventive treatment before the onset of severe, treatment-resistant damages. However, there is a lack of comprehensive investigation of lesions and molecular targets in the entire organ, whereas spatial identification of early-stage lesions is potentially overlooked at the single-cell level.

Clinical Manifestations.

Background: Despite the clinical importance and significant social burden of neuropsychiatric symptoms (NPS) in dementia, the underlying neurobiological mechanism remains poorly understood. Recently, neuroimaging-derived brain-age estimation by machine-learning analysis has shown promise as an individual-level biomarker. We investigated the relationship between NPS and brain-age in amnestic mild cognitive impairment (MCI) and early dementia.

sp. nov., isolated from duckweed and formal proposal to reclassify as a later heterotypic synonym of and reclassify as subsp. .

A novel strain DW16-2, isolated from duckweed (), was taxonomically studied in detail. The analysis based on its 16S rRNA gene sequence revealed that the strain was most closely related to Y8 (98.8%), followed by YIM 61452 (98.

Effects of sex on imaging-based intravenous thrombolysis for ischaemic stroke with unknown onset time: a pooled analysis of clinical trials.

Introduction: The effects of imaging-based intravenous thrombolysis on outcomes based on patient sex remain unclear. We aimed to investigate whether outcomes among patients with stroke with an unknown onset time and treated with imaging-based intravenous thrombolysis are influenced by their sex.

Patients And Methods: This study was a pooled analysis of individual patient-level data acquired from the Evaluation of unknown Onset Stroke thrombolysis trials.

Diet-related quality of life may directly and indirectly affect health-related quality of life through protein intake and frailty in patients with osteoporosis: Results from a prospective cohort study.

Aim: We aimed to examine the direct and indirect associations between diet-related quality of life (DRQOL) and health-related quality of life (HRQOL) in patients with osteoporosis.

Methods: Cross-sectional and longitudinal analyses were conducted using data from a 1-year prospective cohort survey of 532 consecutive patients with osteoporosis. The DRQOL was assessed using a short version of the Diet-Related Quality of Life Scale.

Pre-stroke patient characteristics that influence skeletal muscle quality: A cross-sectional study.

Aim: One of the parameters that is measurable using bioelectrical impedance is the phase angle (PhA), which is an indicator of skeletal muscle quality. The PhA reflects cellular health and nutritional status and is an important parameter for monitoring recovery after stroke. However, the factors affecting skeletal muscle quality remain unclear.

Effectiveness of tacrolimus therapy in refractory ulcerative colitis compared to infliximab with propensity score matching.

There is insufficient evidence comparing the outcomes of tacrolimus-based remission induction therapy with infliximab in refractory ulcerative colitis (UC) and evidence regarding optimal strategies after tacrolimus-based remission induction therapy. We conducted a multi-institutional retrospective study of patients with UC treated with tacrolimus or infliximab between January 2010 and March 2019. The proportion of clinical remission at week 8 and cumulative colectomy-free rate were examined using propensity score matching analysis.

Multispanning membrane protein SIDT2 increases knockdown activity of gapmer antisense oligonucleotides.

Recent advances in the clinical development of oligonucleotide therapeutics, such as antisense oligonucleotides (ASOs) and small interfering RNAs, have attracted attention as promising therapeutic modalities for genetic and intractable diseases. These oligonucleotide therapeutics exert their efficacy by binding to target RNAs present within cells; however, the mechanisms underlying their cellular uptake, especially their passage through membranes, remain largely unclear. In the nematode, Caenorhabditis elegans, the multi-pass transmembrane protein, SID-1, is involved in the cellular uptake of double-stranded RNAs.

Acquisition of similar properties by filters in the same stream of a multistream convolutional neural network.

Functional modular organization is observed in a variety of cortical areas in the brain. In the visual cortex of primates, adjacent neurons often respond to the same visual submodality, such as color or orientation, and have a similar preferred orientation or preferred color. However, it remains unclear why functional modular organization emerges in the cerebral cortex.

Sounds like gambling: detection of gambling venue visitation from sounds in gamblers' environments using a transformer.

Objective digital measurement of gamblers visiting gambling venues is conducted using cashless cards and facial recognition systems, but these methods are confined within a single gambling venue. Hence, we propose an objective digital measurement method using a transformer, a state-of-the-art machine learning approach, to detect total gambling venue visitations for gamblers who visit multiple gambling venues using sounds in gamblers' environments. We sampled gambling and nongambling event datasets from websites to create a gambling play classifier.

Validation of musculoskeletal segmentation model with uncertainty estimation for bone and muscle assessment in hip-to-knee clinical CT images.

Deep learning-based image segmentation has allowed for the fully automated, accurate, and rapid analysis of musculoskeletal (MSK) structures from medical images. However, current approaches were either applied only to 2D cross-sectional images, addressed few structures, or were validated on small datasets, which limit the application in large-scale databases. This study aimed to validate an improved deep learning model for volumetric MSK segmentation of the hip and thigh with uncertainty estimation from clinical computed tomography (CT) images.

Stabilization of oxygen vacancy ordering and electrochemical-proton-insertion-and-extraction-induced large resistance modulation in strontium iron cobalt oxides Sr(Fe,Co)O.

Electrochemically inserting and extracting hydrogen into and from solids are promising ways to explore materials' phases and properties. However, it is still challenging to identify the structural factors that promote hydrogen insertion and extraction and to develop materials whose functional properties can be largely modulated by inserting and extracting hydrogen through solid-state reactions at room temperature. In this study, guided by theoretical calculations on the energies of oxygen reduction and hydrogen insertion reactions with oxygen-deficient perovskite oxides, we demonstrated that the oxygen vacancy ordering in Sr(FeCo)O (SFCO) epitaxial films can be stabilized by increasing the Co content (x ≥ 0.

Excitable Ras dynamics-based screens reveal RasGEFX is required for macropinocytosis and random cell migration.

Excitable systems of eukaryotic chemotaxis can generate asymmetric signals of Ras-GTP-enriched domains spontaneously to drive random cell migration without guidance cues. However, the molecules responsible for the spontaneous signal generation remain elusive. Here, we characterized RasGEFs encoded in Dictyostelium discoideum by live-cell imaging of the spatiotemporal dynamics of Ras-GTP and hierarchical clustering, finding that RasGEFX is primarily required for the spontaneous generation of Ras-GTP-enriched domains and is essential for random migration in combination with RasGEFB/M/U in starved cells, and they are dispensable for chemotaxis to chemoattractant cAMP.

Commentary on: "Structural insights into a bacterial β-glucosidase capable of degrading sesaminol triglucoside to produce sesaminol: toward the understanding of the aglycone recognition mechanism by the C-terminal lid domain".

Sesaminol is an organic compound which shows the strong antioxidant, anti-inflammatory, and neuroprotective properties. Sesaminol triglucoside (STG) is glycosylated form of sesaminol and abundantly exists in sesame seeds. However, typical β-glucosidases could not deglycosylate STG probably due to its bulky aglycone.

Complete genome sequence of Flavobacteriaceae sp. strain GF1, isolated from the phycosphere of a coral endosymbiotic dinoflagellate.

A Flavobacteriaceae sp. strain GF1 was isolated from an endosymbiotic dinoflagellate of a coral, and the genome was sequenced using a PacBio Sequel IIe system. The genome consists of a circular 5,300,001 bp chromosome and is predicted to harbor 6 rRNA genes, 42 tRNA genes, and 4,465 coding sequences.

Salivary chromogranin A levels relate to fatigability after waking up.

This study aimed to assess fatigue using a noninvasive, quantitative, and simple method using salivary chromogranin A (CgA). In total, 171 adults participated in this study. We used the Cornell Medical Index (CMI) as a questionnaire that included a fatigability section.

Synthesis and Characterization of Multifunctional Fluorescent-Magnetic Platinum Nanoclusters.

There is great interest in the development of new multifunctional fluorescent-magnetic nanomaterials for use as multimodal diagnostic imaging probes and site-specific drug delivery tools. Metal nanoclusters (NCs) have been reported to possess either fluorescent or magnetic properties, but not both. In this paper, we report the synthesis and characterization of multifunctional fluorescent-magnetic Pt NCs.

Putting performance bias to the front-lower side of the hole on steep slopes: Differences in strategies and movements between professional and amateur golfers.

When playing on an sloped golf course, the ball often misses the hole to the front-lower side, which is also referred to as the "amateur side." This can be attributed to the difficulty in perceiving the green slope and choosing the appropriate launch direction and ball speed, especially for amateurs. The study had three main objectives.

piRNA processing within non-membrane structures is governed by constituent proteins and their functional motifs.

Discovered two decades ago, PIWI-interacting RNAs (piRNAs) are crucial for silencing transposable elements (TEs) in animal gonads, thereby protecting the germline genome from harmful transposition, and ensuring species continuity. Silencing of TEs is achieved through transcriptional and post-transcriptional suppression by piRNAs and the PIWI clade of Argonaute proteins within non-membrane structured organelle. These structures are composed of proteins involved in piRNA processing, including PIWIs and other proteins by distinct functional motifs such as the Tudor domain, LOTUS, and intrinsic disordered regions (IDRs).

Pain Catastrophizing Is Associated With Health-Related Quality of Life in Patients With Hip Osteoarthritis: A Multicenter Cross-Sectional Study.

Objective: This study aimed to examine the relationship between pain catastrophizing (PC) and health-related quality of life (HRQoL), while accounting for pain intensity and other factors in patients with hip osteoarthritis (OA).

Methods: This multicenter, cross-sectional study included a total of 160 participants and was conducted at five hospitals in Japan. The primary outcome was the HRQoL status, which was assessed using the Japanese version of the 12-item Short Form.

Automated chest CT three-dimensional quantification of body composition: adipose tissue and paravertebral muscle.

This retrospective study developed an automated algorithm for 3D segmentation of adipose tissue and paravertebral muscle on chest CT using artificial intelligence (AI) and assessed its feasibility. The study included patients from the Boston Lung Cancer Study (2000-2011). For adipose tissue quantification, 77 patients were included, while 245 were used for muscle quantification.