Risk factors for skin injuries in cancer patients with peripherally inserted central catheter: A prospective multicenter cohort study.

Background: The risk factors for skin injuries remain poorly understood in cancer patients with peripherally inserted central catheters (PICC). We herein aimed at exploring the effect of clinical factors on the risk of PICC-related skin injuries.

Methods: We included 1245 cancer patients with PICC from 16 hospitals in Suzhou, China.

A transcriptional metabolic gene-set based prognostic signature is associated with clinical and mutational features in head and neck squamous cell carcinoma.

Purpose: Head and neck squamous cell carcinoma (HNSCC) is a common cancer with high mortality and poor prognosis partially owing to lack of application of predictive markers. Increasing evidence has suggested that metabolic dysregulation plays an important part in tumorigenesis. We aim to identify a prognostic metabolic pathway (MP) signature in HNSCC.

Identification of SERPINE1, PLAU and ACTA1 as biomarkers of head and neck squamous cell carcinoma based on integrated bioinformatics analysis.

Background: Head and neck squamous cell carcinoma (HNSCC) is the six leading cancer by incidence worldwide. The 5-year survival rate of HNSCC patients remains less than 65% due to lack of symptoms in the early stage. Hence, biomarkers which can improve detection of HNSCC should improve clinical outcome.

[The role of extracellular signal regulated kinase 1/2 in mediating osteodifferentiation of human periodontal ligament cells induced by cyclic stretch].

Objective: This study aimed to investigate the mechanism of cyclic stretch that promotesthe osteogenic differentiation of human periodontal ligament cells (hPDLCs) through the mediation of extracellular-signal-regulated kinase 1/2 (ERK1/2).

Methods: hPDLCs were isolated through the explant method and cultured in vitro. hPDLCs were mechanically stimulated by a multi-channel cell-stress-loading system for 1, 3, 6, 12, and 24 h.

Long-term effect of ropivacaine nanoparticles for sciatic nerve block on postoperative pain in rats.

Purpose: The analgesic effect of ropivacaine (Rop) for nerve block lasts only ~3-6 hours for single use. The aim of this study was to develop long-acting regional anesthetic Rop nanoparticles and investigate the effects of sciatic nerve block on postoperative pain in rats.

Materials And Methods: Rop nanoparticles were developed using polyethylene glycol-co-polylactic acid (PELA).

Pulsed dye laser therapy for infantile hemangiomas: a systemic review and meta-analysis.

Background: Infantile hemangiomas (IH) are common pediatric tumors. This meta-analysis was performed to review the therapeutic efficacy and safety of pulsed dye laser (PDL) in the treatment of IH.

Methods: Seven databases were searched, including PubMed, OvidSP, Karger, Elsevier, EMBASE, Web of Science and Wiley Online Library.

Non-polio enteroviruses from acute flaccid paralysis surveillance in Shandong Province, China, 1988-2013.

Enteroviruses (EVs) are important human pathogens associated with various clinical syndromes. This study represents an overview of non-polio enteroviruses (NPEVs) isolated from acute flaccid paralysis (AFP) surveillance in Shandong Province, China from 1988 to 2013. Altogether 792 and 170 NPEV isolates were isolated from stool specimens of 9263 AFP cases and 1059 contacts, respectively.

miR-101 sensitizes A549 NSCLC cell line to CDDP by activating caspase 3-dependent apoptosis.

MicroRNA-101 (miR-101) is evidently downregulated in several types of cancer, including non-small cell lung cancer (NSCLC), and is crucial in sensitizing cells to chemotherapy drugs. The aim of the present study was to investigate the correlation between miR-101 and chemosensitivity in the A549 NSCLC cell line. Here, we used the human A549 cell line for transfection with an miR-101 overexpressing vector and detected the cytotoxic acticity, proliferation and apoptosis of -diaminedichloroplatinum (CDDP) in A549-miR-101 and A549-mock cells.

[Mechanism of carbon monoxide affecting the expression of cellular adhesion molecule under stimulation of inflammatory cytokines to human gingival fibroblasts].

Objective: To investigate the mechanism by which carbon monoxide inhibits the expression of adhesion molecules on human gingival fibroblasts (HGF) stimulated with inflammatory cytokines.

Methods: HGF were cultured in vitro, and stimulated with 50 ng x mL tumor necrosis factor-alpha (TNF-alpha) and 10 ng x mL(-1) interleukin-1beta (IL-1beta) concurrently in the presence or absence of carbon monoxide releasing molecule-3 (CORM-3) at 500 micromol x L-1. Expression of phosphorylated extracellular regulated protein kinase (ERK), phosphorylated c-Jun N-terminal kinase (NK) and phosphorylated p38 in mitogen-activated protein kinase(MAPK) pathway was studied by Western blot at 10 min and 20 min, respectively.

Construction of a sIgA-enhancing anti-Porphyromonas gingivalis FimA vaccine and nasal immunization in mice.

Porphyromonas gingivalis is implicated in the etiology of chronic periodontitis. Fimbriae are one of several critical surface virulence factors of P. gingivalis.

[Construction of eukaryotic co-expression plasmid harboring genes encoding Porphyromonas gingivalis fim A and human IL-15].

Objective: To construct a eukaryotic co-expression plasmid pIRES-fimA:IL15, which can be used as an immunoreaction-enhancing DNA vaccine against Porphyromonas gingivalis FimA, and investigate its expression in mammalian cells.

Methods: The eukaryotic co-expression plasmid pIRES-fimA:IL15 was constructed by molecular cloning methods and characterized by restricted endonuclease mapping, PCR and DNA sequencing. The plasmid was transfected into mammalian cell CHO using Lipofectamine 2000.

[The effects of non-surgical periodontal treatment on the formation of root-dentin hypersensitivity].

Purpose: To study the effects of non-surgical periodontal treatment on the occurrence of root-dentin hypersensitivity and explore its mechanisms through clinical observations.

Methods: 1453 teeth of 52 patients with chronic periodontitis were given scaling and root-planing procedures. 3 months follow-up of these teeth were taken to find root-dentin hypersensitivity after treatment.

[The observation of the local anaesthesia effect of primacaine in the treatment of pulpitis].

Purpose: To explore the application and the anaesthetic effect of primacaine in anaesthetizing the pulp.

Methods: 162 teeth of pulpitis underwent submucous infiltration anaesthesia via the labial or buccal side (single side anaesthesia). There were 94 teeth of acute pulpitis and 68 teeth of chronic pulpitis among them.

[Clinical evaluation on effects of Ca(OH)2-glycerol paste for dental canal sterilization].

Objective: Through clinical observation on an formula containing mainly Ca(OH)(2) for dental canal sterilization to confirm it as an ideal canal sterilizer.

Methods: 5% (M/V) of Ca(OH)(2) in glycerol was used as dental canal sterilizer. Patients with acute or chronic apical periodontitis were randomly selected, and their symptoms were recorded before treatment.

[Clinical effects of three systems of dental implants].

Objective: To evaluate the clinical value of dental implants including Brånemark, Steri-oss and BLB systems.

Methods: The total 384 implants of different materials and shapes were inserted normally. According to the Chinese standard of dental implantation, the success rates of three types were compared.