Spectrum of Craniofacial and Oral Malformations in China, a Multicenter Study.

Objective: Craniofacial and oral malformations (COMs) represent an important class of human developmental disorders with profound implications on the anatomical structure, appearance, and various physiological functions. In this study, we aimed to define the spectrum of COMs and analysis its features or possible influencing factors to improve the surveillance and control of the disease.

Materials And Methods: We organized a multicenter survey of COMs from 19 hospitals in 14 provinces.

Abnormal expression and related regulatory mechanism of long noncoding RNA in head and neck squamous cell carcinoma.

Objective: The objective of this study was to investigate the effect and mechanism of long noncoding RNA (lncRNA) on head and neck squamous cell carcinoma (HNSCC).

Study Design: RNA was extracted from HNSCC tissue and adjacent tissues and sequenced. Differentially expressed lncRNA and microRNA (miRNA) were screened.

A novel EDAR variant identified in non-syndromic tooth agenesis: Insights from molecular dynamics.

Objective: This study aims to investigate a novel pathogenic variant in a Chinese family of non-syndromic tooth agenesis (NSTA) and study the impact of the variant on related protein and pathway.

Design: One NSTA family was collected. Whole exome sequencing and Sanger sequencing were performed on the proband with NSTA and his 5 family members.

Quality of life in patients with cancer-related Brown IIb maxillary defect: A comparison between conventional obturation rehabilitation and submental flap reconstruction.

Objectives: The purpose of this retrospective study was to compare the differences in quality of life (QOL) outcomes between the conventional obturator prostheses (COP) and the pedicled submental artery island flap (SAIF) in the reconstruction of Brown IIb maxillary defects.

Materials And Methods: The QOL of 116 eligible patients who had a lapse ≥ 12 months after the cancer-related maxilla ablation was evaluated by the University of Washington quality of life scale (UW-QOL), Performance Status Scale for Head and Neck (PSS-HN), and Obturator Functioning Scale (OFS).

Results: Patients in the SAIF group reported statistically and clinically significant higher overall QOL scores but lower chewing scores in the UW-QOL scale when compared with those in the COP group (P < 0.

Effect of 17β-estradiol on the proliferation of condylar chondrocytes.

Objectives: To study the effects of 17β-estradiol (E2) on the regulation of the proliferation of condylar chondrocytes and provide a preliminary discussion on the role of phosphorylate-mammalian target of rapamycin (p-mTOR) in this regulatory process.

Methods: Condylar chondrocytes were isolated from 6-week-old female rats for primary culture. Drug treatment with different concentrations of E2 and/or rapamycin (RAPA) was carried out on second-generation cells.

[ and anti-periodontitis effects of combination treatment of photodynamic and antibiotic therapies].

To investigate the effects of combination treatment of photodynamic therapy (PDT) based on photosensitizer chlorin e6 (Ce6) and antibiotic agent tinidazole (TNZ) against periodontitis both and . The Sprague-Dewley (SD) rat periodontitis model was constructed using the method of orthodontic wire ligation. After successful modeling, SD rats were randomly divided into the following 6 groups (3 rats in each group): positive control (Ctrl+), Ce6, TNZ, a mixture of Ce6 and TNZ (Ce6/TNZ), Ce6 with laser irradiation (Ce6+L), a mixture of Ce6 and TNZ with laser irradiation (Ce6/TNZ+L).

[Expression and distribution of calprotectin in healthy and inflamed periodontal tissues].

Objective: Calprotectin, the heterdimer of S100A8 and S100A9, is the major cytoplasmic protein of neutrophils, which is also expressed or induced in gingival epithelial cells, activated mononuclear macrophages and vascular endothelial cells. Calprotectin is intimately associated with the initiation and progression of periodontitis, but the expression patterns of calprotectin in healthy and inflamed periodontal tissue are not fully understood. To observe the expression, distribution and cellular localization of calprotectin in the samples of healthy periodontal tissues and experimental periodontitis tissues of Beagles and to explore their relationship with periodontal inflammation and possible effect.

Scientometric Analysis of Dental Implant Research over the Past 10 Years and Future Research Trends.

Background: We conducted a bibliometrics analysis to explore the recent trends in dental implant research which could help researchers have a clear grasp of the relevant research hotspots and prospects. . Altogether, 15,770 articles on dental implants, from January 1, 2010, to October 31, 2019, were selected from the Web of Science Core Collection.

Effects and Mechanism of Noninvasive Positive-Pressure Ventilation in a Rat Model of Heart Failure Due to Myocardial Infarction.

BACKGROUND Impaired heart function induced by myocardial infarction is a leading cause of chronic heart failure (HF). This study aimed to investigate the effects and mechanism of noninvasive positive-pressure ventilation (NIPPV) in a rat model of HF due to myocardial infarction. MATERIAL AND METHODS To explore the therapeutic effect and mechanism of NIPPV on acute myocardial infarction-induced HF, we established a rat model of HF by ligating the anterior descending branch of the left coronary artery and confirmed by ultrasonic cardiography and brain natriuretic peptide 45 detection.

[Comparison of two types of T-shaped coronectomy applied in extraction of impacted mandibular third molars].

Purpose: To compare the clinical efficiency of two types of T-shaped coronectomy applied in extraction of impacted mandibular third molars. METHODS：One hundred and twenty patients meeting with the criteria were randomly divided into 2 groups. Two types of T-shaped coronectomy were applied in extraction of impacted mandibular third molars.

Role of the Calcified Cartilage Layer of an Integrated Trilayered Silk Fibroin Scaffold Used to Regenerate Osteochondral Defects in Rabbit Knees.

The repair of osteochondral defects remains challenging, given the complexity of native osteochondral tissue and the limited self-repair capacity of cartilage. Osteochondral tissue engineering is a promising strategy. Here, we fabricated a biomimetic osteochondral scaffold using silk fibroin and hydroxyapatite, including a calcified cartilage layer (CCL).

Oncogenic HPV promotes the expression of the long noncoding RNA lnc-FANCI-2 through E7 and YY1.

Long noncoding RNAs (lncRNAs) play diverse roles in biological processes, but their expression profiles and functions in cervical carcinogenesis remain unknown. By RNA-sequencing (RNA-seq) analyses of 18 clinical specimens and selective validation by RT-qPCR analyses of 72 clinical samples, we provide evidence that, relative to normal cervical tissues, 194 lncRNAs are differentially regulated in high-risk (HR)-HPV infection along with cervical lesion progression. One such lncRNA, , is extensively characterized because it is expressed from a genomic locus adjacent to the gene encoding an important DNA repair factor.

Cartilage Extracellular Matrix Scaffold With Kartogenin-Encapsulated PLGA Microspheres for Cartilage Regeneration.

Repair of articular cartilage defects is a challenging aspect of clinical treatment. Kartogenin (KGN), a small molecular compound, can induce the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) into chondrocytes. Here, we constructed a scaffold based on chondrocyte extracellular matrix (CECM) and poly(lactic-co-glycolic acid) (PLGA) microspheres (MP), which can slowly release KGN, thus enhancing its efficiency.

Immunomodulation of MSCs and MSC-Derived Extracellular Vesicles in Osteoarthritis.

Osteoarthritis (OA) has become recognized as a low-grade inflammatory state. Inflammatory infiltration of the synovium by macrophages, T cells, B cells, and other immune cells is often observed in OA patients and plays a key role in the pathogenesis of OA. Hence, orchestrating the local inflammatory microenvironment and tissue regeneration microenvironment is important for the treatment of OA.

Effectiveness of cognitive behavioral therapy on kinesiophobia and oral health-related quality of life in patients with temporomandibular disorders, study protocol for a randomized controlled trial.

Background: Temporomandibular disorders (TMD) is a common physical and psychological disease in dental department. Pain and mandibular limitation are the main reasons for patients to seek oral treatment. However, the presence of kinesiophobia, patients often catastrophize pain, so as to avoid mandibular movement, which seriously affects their quality of life.

Discovery of a Chinese familial deletion 18p syndrome due to a false positive result on noninvasive prenatal testing.

Clinical manifestations of deletion 18p syndrome vary a lot, which makes it easily overlooked in the clinical practice. Familial transmission of deletion 18p syndrome is rare. We report a Chinese familial deletion 18p syndrome, which was diagnosed by anatomizing the underlying reason for the discrepancy between noninvasive prenatal testing (NIPT) and prenatal diagnosis.

A novel missense mutation of LRP6 identified by whole-exome sequencing in a Chinese family with non-syndromic tooth agenesis.

Objective: The aim of this study was to explore the genetic basis of non-syndromic tooth agenesis (TA) in a Chinese family of five individuals using whole-exome sequencing (WES) analysis.

Settings And Sample Population: Five participants/Family-based study of a non-syndromic TA proband.

Methods: The proband, proband's mother and grandmother displayed congenital tooth deficiency.

[Analysis of key genes and signal pathways of human papilloma virus-related head and neck squamous cell carcinoma].

To explore differentially expressed genes (DEG) and pathways between human papilloma virus (HPV) positive and negative head and neck squamous cell carcinoma (HNSCC) and to search gene targets for diagnosis and treatment of HPV-related HNSCC. HPV-related HNSCC expression profile chips of GSE3292 (including 8 HPV-positive and 28 HPV-negative HNSCC tissues, of which 15 collected from oral cavity cancer, 9 from oropharyngeal cancer, 9 from laryngeal cancer and 3 from hypopharyngeal cancer) were selected from Gene Expression Omnibus (GEO) database of National Center for Biotechnology Information and DEG were screened out using Gene-Cloud of Biotechnology Informs (GCBI). Gene ontology and pathway enrichment analysis were performed using DAVID and protein-to-protein interaction (PPI) network was constructed by STRING.

Current Trends in Research on Bone Regeneration: A Bibliometric Analysis.

Background: Bone regeneration is a frequent research topic in clinical studies, but macroscopic studies on the clinical application of bone regeneration are rare. We conducted a bibliometric analysis, using international databases, to explore the clinical application and mechanism of bone regeneration, to highlight the relevant research hotspots and prospects. .

Synergistic Effects of Kartogenin and Transforming Growth Factor-β3 on Chondrogenesis of Human Umbilical Cord Mesenchymal Stem Cells In Vitro.

Objective: To explore the effect of kartogenin (KGN) on proliferation and chondrogenic differentiation of human umbilical cord mesenchymal stem cells (hUCMSC) in vitro, and the synergistic effects of KGN and transforming growth factor (TGF)-β3 on hUCMSC.

Methods: Human umbilical cord mesenchymal stem cells were isolated and cultured. Then the differentiation properties were identified by flow cytometry analysis.

Phase evolution, mechanical properties and MRI contrast behavior of GdPO doped hydroxyapatite for dental applications.

Ca(PO)(OH) doped with GdPO (HA-GP) was synthesized by a sol-gel technique, and its phase composition depended on the GdPO content. At low doping levels, the phase structure was composed of hydroxyapatite and some Ca(PO), and the content of the latter decreased with the doping content; when the doping content reached to 15 mol%, however, the Ca(PO) amount revealed a significant increase, and GdPO turned to be precipitated. With the addition of GdPO, the microstructure of HA-GP pellets became dense, and the grain size decreased.

[Research progress in zygomatic implant technique].

Zygomatic implant technique is an effective alternative for the prosthetic rehabilitation of maxilla with severe bone defect exteriorly and functionally, which not only avoid bone grafting and shorten the prosthetic process in some way, but also improve the prognosis quality of patient, as well as the pronunciation and the chew function. With the popularity in the clinic, zygomatic implants have been developed these years. This article will give a systematical introduction including the update of the application of the technology, as well as the selection of the quantity and location of the implants, the modifications of the operation technique, the avoidance and solution of common specific complications, the establishment of zygomatic implant index and the assistance of the computerized technique in the operation.